FUNCTIONAL INTERACTION BETWEEN caf5 AND SPERMIDINE FAMILY TRANSPORTERS IN S. POMBE

Yıl 2019, Cilt: 8 Sayı: 2, 170 - 178, 31.07.2019

Aslıhan Örs Gevrekci

https://doi.org/10.18036/estubtdc.598756

Öz

ABSTRACT

 Polyamines are well known for their wide range of
roles in the cells, including stabilization of nucleic acids, regulation of
gene expression at different levels, stress response and cell cycle. The
activity of these well conserved small polycations are also regulated by
different mechanisms to assure their intracellular concentration and activity.
Polyamine transporters have a major role in the regulation of the influx and
efflux of the polyamines. Previously, two spermine family transporters SPBC409.08
and caf5 were characterized which were associated with cell size
defects. The aim of this study is to better understand the role of caf5 plasma
membrane spermine transporter and its functional interaction with spermidine
transporters. Double mutants of caf5  and spermidine family transporters SPBC36.01c,
SPBC36.03c and SPBC569.05c were formed in S. pombe. The
double mutants were then put through growth rate analysis, meiotic progression
and sporulation analysis, cell cycle analysis, and vulnerability to environmental
stress. The results showed that the double mutants differentiate from each
other in their combined mutation with caf5 in terms of cell size and DNA
damage response, indicating redundant seperate pathways of spermine and
spermidine transport in S. pombe.

Anahtar Kelimeler

S. pombe, Cell Size, Cell Cycle, Stress Response, Polyamines

Kaynakça

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