IN VITRO BIOLOGICAL ACTIVITY EVALUATION OF ETHANOLIC EXTRACT OF MELICA UNIFLORA LEAVES

Yıl 2021, Cilt: 10 Sayı: 1, 17 - 26, 25.01.2021

Elif Çil , Sevda Türkiş , Melek Çol Ayvaz , Ceren Börçek Kasurka

https://doi.org/10.18036/estubtdc.587101

Cited By: 2

Öz

Melica uniflora is part of the omnivore and
herbivore animal diets and there is not enough study about this plant. The aim
of the study is to provide the antimicrobial and antioxidant potential of
ethanol extract of M. uniflora leaves. 
Total phenolic and
flavonoid contents and antioxidant potential based on different methods and
antimicrobial activity of M. uniflora leaves ethanol extract were examined.
Disk diffusion method against to
eight bacteria strains, the Folin-Ciocalteu
method, DPPH free radical scavenging activity and ferric reducing
antioxidant power assays were used. Mean diameters of inhibition zones were found in the range of
14.93 mm to 26.32 mm.
The total phenolic content of ethanol extract of M. uniflora leaves was
determined as 0.466 mg GAE/g. Total antioxidant activity value of the extract was
calculated 4.54 mg AAE/g. IC₅₀
value was calculated 18.798 mg/mL for DPPH free radical scavenging activity.
The FRAP value indicated that the reducing
power of 1 gram of sample was equivalent to 3.33 µmol of trolox. The flavonoid
content of the extract was calculated as
4.44 mgQE/g. According to the obtained results, the analyzed M.
uniflora leaves ethanol extracts demonstrated antioxidant and antimicrobial
activity level can be considered effective. 

Anahtar Kelimeler

Wood melick, forage selection, Yenice Forest, forest health, Nocardia

Destekleyen Kurum

Ordu University

Proje Numarası

Scientific Research Project No: HD 1707

Teşekkür

The authors gratefully acknowledge financial support through and would like to thank the Ordu University (Scientific Research Project No: HD 1707).

MELICA UNIFLORA YAPRAKLARININ ETANOLİK ÖZÜTLERİNİN IN VITRO BİYOLOJİK AKTİVİTESİNİN DEĞERLENDİRİLMESİ

Öz

Melica
uniflora, omnivor ve otobur hayvan diyetlerinin bir
parçasıdır ve bu bitki hakkında yeterli çalışma yoktur. Çalışmanın amacı, M. uniflora yapraklarının etanol
ekstraktının antimikrobiyal ve antioksidan potansiyelini belirlemektir. M. uniflora yapraklarının etanol
özütlerinin antimikrobiyal aktiviteleri ile toplam fenolik ve flavonoid
içerikleri ve antioksidan potansiyeli farklı yöntemlere dayanarak
incelenmiştir. Çalışmada sekiz bakteri suşuyla disk difüzyon metodu,
FolinCiocalteu metodu, DPPH serbest radikal temizleme aktivitesi ve ferrik
indirgeyici antioksidan güç analizleri kullanılmıştır. Ortalama inhibisyon zon
çapları 14.93 mm ile 26.32 mm arasında bulunmuştur. M. uniflora yapraklarının etanol ekstraktının toplam fenolik
içeriği 0.466 mg GAE/g olarak belirlenmiştir. Ekstrenin toplam antioksidan
aktivite değeri 4.54 mg AAE/g olarak hesaplanmıştır. IC₅₀ değeri
DPPH serbest radikal süpürme aktivitesi için 18.798 mg/mL olarak hesaplanmıştır.
FRAP değeri, 1 gram numunenin indirgeme gücünün 3.33 umol troloks'a eşdeğer
olduğunu göstermiştir. Ekstrenin flavonoid içeriği 4.44 mgQE/g olarak
hesaplanmıştır. Elde edilen sonuçlara göre, analiz edilen M. uniflora etanol özütlerinin antioksidan ve antimikrobiyal
aktivite gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Seyrek inci otu, yem seçimi, Yenice Ormanı, orman sağlığı, Nocardia

Proje Numarası

Scientific Research Project No: HD 1707

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