Araştırma Makalesi
PDF Zotero Mendeley EndNote BibTex Kaynak Göster

IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Yıl 2021, Cilt 10, Sayı 2, 132 - 137, 30.07.2021
https://doi.org/10.18036/estubtdc.802794

Öz

This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.

Kaynakça

  • [1] Abou-Seif MA, Youssef AA. Evaluation of some biochemical changes in diabetic patients. Clin Chim Acta, 2004;346(2):161-170.
  • [2] Andian G, Burçak G. Oxidative damage to nuclear DNA in streptozotocin-diabetic rat liver. Clin Exp Pharmacol Physiol, 2005;32(8):663-666.
  • [3] ADA (American Diabetes Association). Diagnosis and classification of diabetes mellitus. Diabet Care, 2011;34(1):62-69.
  • [4] Bildik A, Yur F, H Camas, Dede S. Investigation of glutathione and lipid peroxidation levels with respect to haemoglobin types in lambs with white muscle disease. Van Vet J, 1996;7(1-2):95-98.
  • [5] Franco R, Schoneveld OJ, Pappa A, Panayiotidis MI. The central role of glutathione in the pathophysiology of human diseases. Arch Physiol Biochem, 2007;113:243-258.
  • [6] Dede S, Çamaş H. Determination of some biochemical parameters (GSH, Hb and Tf types and Mn) in laying hens and study its effects on egg production. YYU Sag Bil Derg, 2001;7:131-138.
  • [7] Ulukaya E, Pirianov G, Kurt MA, Wood EJ, Mehmet H. Fenretinide induces cytochrome C release, caspase 9 activation and apoptosis in the absence of mitochondrial membrane depolarisation. Cell Death Different, 2003;10:856-859.
  • [8] Danial NN, Korsmeyer SJ. Cell death: critical control points. Cell, 2004;11:205-219.
  • [9] Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol, 2007;35:495-516.
  • [10] Schultz-Johanssen J, Harris AK, Rychly DJ, Ergul A. Oxidative stress and the use of antioxidants in diabetes. linking basic science to practice. Cardiovasc Diabetol, 2005;4-5.
  • [11] Yur F, Dede S, Karaca T, CiftçiYegin S, Değer Y, Ozdemir H. The effect of glutathione treatment on the biochemical and immunohistochemical profile in streptozotocin-induced diabetic rats. J Membrane Biol, 2013;246(6):427-433.
  • [12] Yur F, Dede S, Taşpınar M, Usta A, Cetin S. Effects of glutathione application on oxidative DNA damage and antioxidant system in the glucose-treated kidney cell line. YYU BAP, (unpublished data), Project No:2013-VF-B062.
  • [13] Aksoy Y. The role of glutathione in antioxidant mechanism. Turk J Med Sci, 2002; 2(4):442-448.
  • [14] Bonnefont-Rousselot D. Glucose and reactive oxygen species. Curr Opin Clin Nutr Metab Care, 2002;5:561-568.
  • [15] Sekhar RV, McKay SV, Patel SG, Guthikonda AP, Reddy VT, Balasubramanyam A et al. Glutathione synthesis is diminished in patients with uncontrolled diabetes and restored by dietary supplementation with cysteine and glycine. Diabetes Care, 2011;34(1):162-167.
  • [16] Kuchake VG, Upasani CD. Effect of Vitamin E and C plus reduced glutathione in treatment of diabetic nephropathy. Int J Pharm Sci Rev Res, 2013;2(12):1-5.
  • [17] Sahin E, Gocmen AY, Koçak, Tuncer M, Gumuslu S. The association of advanced glycation end-products with glutathione status. Ann Clin Biochem, 2008;45(Pt 4):369-374.
  • [18] Tan H, Qurashi A, Poidevin M, Nelson DL, Li H, Jin P. Retrotransposon activation contributes to fragile X premutation RCGG-mediated neurodegeneration. Hum Mol Genet, 2012;21(1):57-65.
  • [19] Schutte B, Henfling M, Kölgen W, Bouman M, Meex S, Leers MPG et al. Keratin 8/18 break down and reorganization during apoptosis. Exp Cell Res, 2004;297(1):11-26.
  • [20] Prabhakar S, Starnes J, Shi S, Lonis B, Tran R. Diabetic nephropathy is associated with oxidative stress and decreased renal nitric oxide production. J Am Soc Nephrol, 2007;18(11):2945-2952.
  • [21] Park A, Suzuki T, Lennarz WJ. Identification of proteins that interact with mammalian peptide: n-glycanase and implicate this hydrolase in the proteasome-dependent pathway for protein degradation. Proc Nation Acad Sci, 2001;98(20):11163-11168.
  • [22] Ueno Y, Kizaki M, Nakagiri RKamiya T, Sumi H, Osawa T. Dietary glutathione protects rats from diabetic nephropathy and neuropathy. J Nutr 2002;132(5):897-900.

IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Yıl 2021, Cilt 10, Sayı 2, 132 - 137, 30.07.2021
https://doi.org/10.18036/estubtdc.802794

Öz

This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.

Kaynakça

  • [1] Abou-Seif MA, Youssef AA. Evaluation of some biochemical changes in diabetic patients. Clin Chim Acta, 2004;346(2):161-170.
  • [2] Andian G, Burçak G. Oxidative damage to nuclear DNA in streptozotocin-diabetic rat liver. Clin Exp Pharmacol Physiol, 2005;32(8):663-666.
  • [3] ADA (American Diabetes Association). Diagnosis and classification of diabetes mellitus. Diabet Care, 2011;34(1):62-69.
  • [4] Bildik A, Yur F, H Camas, Dede S. Investigation of glutathione and lipid peroxidation levels with respect to haemoglobin types in lambs with white muscle disease. Van Vet J, 1996;7(1-2):95-98.
  • [5] Franco R, Schoneveld OJ, Pappa A, Panayiotidis MI. The central role of glutathione in the pathophysiology of human diseases. Arch Physiol Biochem, 2007;113:243-258.
  • [6] Dede S, Çamaş H. Determination of some biochemical parameters (GSH, Hb and Tf types and Mn) in laying hens and study its effects on egg production. YYU Sag Bil Derg, 2001;7:131-138.
  • [7] Ulukaya E, Pirianov G, Kurt MA, Wood EJ, Mehmet H. Fenretinide induces cytochrome C release, caspase 9 activation and apoptosis in the absence of mitochondrial membrane depolarisation. Cell Death Different, 2003;10:856-859.
  • [8] Danial NN, Korsmeyer SJ. Cell death: critical control points. Cell, 2004;11:205-219.
  • [9] Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol, 2007;35:495-516.
  • [10] Schultz-Johanssen J, Harris AK, Rychly DJ, Ergul A. Oxidative stress and the use of antioxidants in diabetes. linking basic science to practice. Cardiovasc Diabetol, 2005;4-5.
  • [11] Yur F, Dede S, Karaca T, CiftçiYegin S, Değer Y, Ozdemir H. The effect of glutathione treatment on the biochemical and immunohistochemical profile in streptozotocin-induced diabetic rats. J Membrane Biol, 2013;246(6):427-433.
  • [12] Yur F, Dede S, Taşpınar M, Usta A, Cetin S. Effects of glutathione application on oxidative DNA damage and antioxidant system in the glucose-treated kidney cell line. YYU BAP, (unpublished data), Project No:2013-VF-B062.
  • [13] Aksoy Y. The role of glutathione in antioxidant mechanism. Turk J Med Sci, 2002; 2(4):442-448.
  • [14] Bonnefont-Rousselot D. Glucose and reactive oxygen species. Curr Opin Clin Nutr Metab Care, 2002;5:561-568.
  • [15] Sekhar RV, McKay SV, Patel SG, Guthikonda AP, Reddy VT, Balasubramanyam A et al. Glutathione synthesis is diminished in patients with uncontrolled diabetes and restored by dietary supplementation with cysteine and glycine. Diabetes Care, 2011;34(1):162-167.
  • [16] Kuchake VG, Upasani CD. Effect of Vitamin E and C plus reduced glutathione in treatment of diabetic nephropathy. Int J Pharm Sci Rev Res, 2013;2(12):1-5.
  • [17] Sahin E, Gocmen AY, Koçak, Tuncer M, Gumuslu S. The association of advanced glycation end-products with glutathione status. Ann Clin Biochem, 2008;45(Pt 4):369-374.
  • [18] Tan H, Qurashi A, Poidevin M, Nelson DL, Li H, Jin P. Retrotransposon activation contributes to fragile X premutation RCGG-mediated neurodegeneration. Hum Mol Genet, 2012;21(1):57-65.
  • [19] Schutte B, Henfling M, Kölgen W, Bouman M, Meex S, Leers MPG et al. Keratin 8/18 break down and reorganization during apoptosis. Exp Cell Res, 2004;297(1):11-26.
  • [20] Prabhakar S, Starnes J, Shi S, Lonis B, Tran R. Diabetic nephropathy is associated with oxidative stress and decreased renal nitric oxide production. J Am Soc Nephrol, 2007;18(11):2945-2952.
  • [21] Park A, Suzuki T, Lennarz WJ. Identification of proteins that interact with mammalian peptide: n-glycanase and implicate this hydrolase in the proteasome-dependent pathway for protein degradation. Proc Nation Acad Sci, 2001;98(20):11163-11168.
  • [22] Ueno Y, Kizaki M, Nakagiri RKamiya T, Sumi H, Osawa T. Dietary glutathione protects rats from diabetic nephropathy and neuropathy. J Nutr 2002;132(5):897-900.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Bölüm Makaleler
Yazarlar

Gülay YAYCI Bu kişi benim
YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ
0000-0002-2936-4583
Türkiye


Semiha DEDE (Sorumlu Yazar)
Van Yüzüncü Yıl Üniversitesi
0000-0001-5744-6327
Türkiye


Ayşe USTA
YÜZÜNCÜ YIL ÜNİVERSİTESİ, FEN FAKÜLTESİ
0000-0002-5522-3469
Türkiye


Veysel YÜKSEK
YÜZÜNCÜ YIL ÜNİVERSİTESİ, ÖZALP MESLEK YÜKSEKOKULU
0000-0001-7432-4989
Türkiye


Sedat ÇETİN
YÜZÜNCÜ YIL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ
0000-0002-6102-8571
Türkiye

Destekleyen Kurum Van Yüzüncü yıl üniversitesi
Proje Numarası 2015-SBE-YL208
Yayımlanma Tarihi 30 Temmuz 2021
Yayınlandığı Sayı Yıl 2021, Cilt 10, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { estubtdc802794, journal = {Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2667-4203}, address = {btdc@anadolu.edu.tr}, publisher = {Eskişehir Teknik Üniversitesi}, year = {2021}, volume = {10}, pages = {132 - 137}, doi = {10.18036/estubtdc.802794}, title = {IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION}, key = {cite}, author = {Yaycı, Gülay and Dede, Semiha and Usta, Ayşe and Yüksek, Veysel and Çetin, Sedat} }
APA Yaycı, G. , Dede, S. , Usta, A. , Yüksek, V. & Çetin, S. (2021). IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION . Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji , 10 (2) , 132-137 . DOI: 10.18036/estubtdc.802794
MLA Yaycı, G. , Dede, S. , Usta, A. , Yüksek, V. , Çetin, S. "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION" . Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 10 (2021 ): 132-137 <https://dergipark.org.tr/tr/pub/estubtdc/issue/64316/802794>
Chicago Yaycı, G. , Dede, S. , Usta, A. , Yüksek, V. , Çetin, S. "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 10 (2021 ): 132-137
RIS TY - JOUR T1 - IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION AU - Gülay Yaycı , Semiha Dede , Ayşe Usta , Veysel Yüksek , Sedat Çetin Y1 - 2021 PY - 2021 N1 - doi: 10.18036/estubtdc.802794 DO - 10.18036/estubtdc.802794 T2 - Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 132 EP - 137 VL - 10 IS - 2 SN - 2667-4203- M3 - doi: 10.18036/estubtdc.802794 UR - https://doi.org/10.18036/estubtdc.802794 Y2 - 2021 ER -
EndNote %0 Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION %A Gülay Yaycı , Semiha Dede , Ayşe Usta , Veysel Yüksek , Sedat Çetin %T IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION %D 2021 %J Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2667-4203- %V 10 %N 2 %R doi: 10.18036/estubtdc.802794 %U 10.18036/estubtdc.802794
ISNAD Yaycı, Gülay , Dede, Semiha , Usta, Ayşe , Yüksek, Veysel , Çetin, Sedat . "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 10 / 2 (Temmuz 2021): 132-137 . https://doi.org/10.18036/estubtdc.802794
AMA Yaycı G. , Dede S. , Usta A. , Yüksek V. , Çetin S. IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2021; 10(2): 132-137.
Vancouver Yaycı G. , Dede S. , Usta A. , Yüksek V. , Çetin S. IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2021; 10(2): 132-137.
IEEE G. Yaycı , S. Dede , A. Usta , V. Yüksek ve S. Çetin , "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION", Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, c. 10, sayı. 2, ss. 132-137, Tem. 2021, doi:10.18036/estubtdc.802794