Histological Disruptive Effects of Tau-Fluvalinate in Zebrafish (Danio rerio) Testis

Öz

Due to the high use of pesticides, undesirable effects are observed in the environment and toxic effects occur in terrestrial and aquatic organisms via pesticide bioaccumulation. Although bioaccumulation levels are low, especially in aquatic ecosystems, toxicological effects on aquatic organisms have been observed. Synthetic pyrethroids are pesticides produced for domestic and agricultural pests and tau-fluvalinate is also a broad-spectrum synthetic pyrethroid. Our study investigated the histopathological effects of zebrafish exposed to tau-fluvalinate at two different doses (8µg/L,16µg/L) on testicular tissue. After 5 days of exposure, testicular tissues were dissected and routine histological methods were applied. Tissues were investigated under a light microscope after they were stained with hematoxylin & eosin. As a result of tau-fluvalinate exposure, deterioration in seminiferous tubule morphology, interstitial fibrosis, vascular congestion, karyorrhexis and hypertrophic spermatogenic cells were detected. Tau-fluvalinate exposure was found to induce apoptosis and cause germ cell deterioration.

Anahtar Kelimeler

• Tau-fluvalinate
• pesticide
• histology
• testis
• zebrafish

Histolopathological Effects of Tau-Fluvalinate in Zebrafish (Danio rerio) Testis

Öz

Due to the high use of pesticides, undesirable effects are observed in the environment and toxic effects occur in terrestrial and aquatic organisms via pesticide bioaccumulation. Although bioaccumulation levels are low, especially in aquatic ecosystems, toxicological effects on aquatic organisms have been observed. Synthetic pyrethroids are pesticides produced for domestic and agricultural pests and tau-fluvalinate is also a broad-spectrum synthetic pyrethroid. Our study investigated the histopathological effects of zebrafish exposed to tau-fluvalinate at two different doses (8µg/L,16µg/L) on testicular tissue. After 5 days of exposure, testicular tissues were dissected and routine histological methods were applied. Tissues were investigated under a light microscope after they were stained with hematoxylin & eosin. As a result of tau-fluvalinate exposure, deterioration in seminiferous tubule morphology, interstitial fibrosis, vascular congestion, karyorrhexis and hypertrophic spermatogenic cells were detected. Tau-fluvalinate exposure was found to induce apoptosis and cause germ cell deterioration.

Anahtar Kelimeler

• Tau-fluvalinate
• pesticide
• histology
• testis
• zebrafish

Kaynakça

1. R. Chadran, A.A. Sivakumar, S. Mohandass, M. Aruchami, “Effect of Cadmium and Zinc on Antioxidant Enzyme Activity in the Gastropod, Achatina fulica”, Comp. Biochem Phsiol., 140C: 422-426, 2005.
2. N. Delen, “Fungisitler”, Nobel Yayın Dağıtım, 552 s. 2008.
3. N. Vural, “Toksikoloji”, A.Ü. Basımevi, 221 s. 2000.
4. L.B. Smith, Kasai S., J.G. Scott, “Pyrethroid resistance in Aedes aegyptiand Aedes albopictus: Important mosquito vectors of human diseases. Pestic. Biochem. Physiol., 133: 1-12, 2016.
5. O. Güntay, H. Çay, B. Durusel, Y. Terzi, “Senteik Piretroidlere Genel Bakış.” Atatürk Univ. J. of Agricultural Faculty, 52 (2): 201-223, 2021.
6. T.R. Roberts, D.H. Hutson, “Metabolic Pathways of Agrochemicals”, Royal Society of Chemistry, 670, 1999.
7. EPA, United States Environmental Protection Agency https://comptox.epa.gov/dashboard/dsstoxdb/results?search=DTXSID7024110&abbreviation=IRIS#exec_sum 01.10.2021
8. EPA, Office of Pesticide Programs, What is pesticide? http://www.epa.gov/pesticides/about, 2005

9. M. Yalçınkaya “Fluvalinate'ın lebistes balıkları (Poecilia reticulata) üzerine akut etkisinin belirlenmesi”, Hacettepe Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 2013.
10. F. Kayhan, G. Kaymak, H. E. Esmer Duruel and Ş. Tartar Kızılkaya, "Biyolojik Araştırmalarda Zebra Balığının (Danio rerio Hamilton, 1822) Kullanılması ve Önemi", Gaziosmanpaşa Bilimsel Araştırma Dergisi, 7(2): 38-45, 2018.
11. K. Bambino, J. Chu, “Zebrafish in Toxicology and Environmental Health”, Curr Top Dev Biol., 124:331-367, 2017.
12. V. Blazer, “Histopathological assessment of gonadal tissue in wild fishes”, Fish Physiology and Biochemistry, 26: 85–101, 2002.
13. P.M. Costa, “The Handbook of Histopathological Practices in Aquatic Environments: Guide to Histology for Environmental Toxicology”, Academic Press, p277, 2018.
14. A.K. Mishra, B. Mohanty, “Acute toxicity impacts of hexavalent chromium on behavior and histopathology of gill, kidney and liver of the freshwater fish, Channa punctatus (Bloch)”, Environ Toxicol Pharmacol., (26): 136-41, 2008.
15. S.A. Sakr, A.E. Azab, “Effect of pyrethroid inhalation on the testis of albino rat”, Pakistan Journal of Biological Sciences, 4(4): 498-500. 2001.
16. Ö.F. Tekbaş, “Kimyasallar ve Üreme Sağlığı”, TSK Koruyucu Hekimlik Bülteni, 5 (1), 2006.
17. P. Sharma, R. Singh, M. Jan, “Dose-Dependent Effect of Deltamethrin in Testis, Liver, and Kidney of Wistar Rats”, Toxicol Int., 21(2): 131–139, 2014.
18. U. Habeeba, M. David, “Histological Respoense In a Common Carp Cyprinus carpio Induced by Sublethal Concentration of Syntetic Pypretroid Lambda Cyhalothrin”, International Journal of Pharmaceutical Chemical and Biological Science, 10(3): 58-64, 2020.
19. A.M.A. Hameed, H.S. Mahmoud, “Cypermethrin induced apoptosis and testicular toxicity by upregulation of p53 in the brain and testis of male rats is alleviated by Sesame oil”, Journal of Taibah University for Science, 14(1), 2020.
20. H.M. Dutta, H.J.M. Meijer, “Sublethal effects of diazinon on the structure of the testis of bluegill, Lepomis macrochirus: a microscopic analysis”, Environmental Pollution, 125: 335-360, 2003.
21. H.M. Dutta, D. Misquitta, K. Sanaullah, “The effects of Endosulfan on the testes of Bluegill fish, Lepomis macrochirus: a histopathological study”, Archives of Environmental Contamination and Toxicology, 51:149-156, 2006.
22. R.K. Srivastava, K.K. Yadav, S.P. Trivedi, “Devicyprin induced gonadal impairment in a freshwater food fish, Channa punctatus (Bloch)”, Journal of Environmental Biology, 29(2):187-191, 2008.
23. A.C. Staicu, M.C. Munteanu, D. Costin, M. Costache, A. Dinischiotu, “Histological changes in deltamethrin- induced intoxication in Carassius auratus gibelio (Pisces- Cyprinidae)”, Biotechnol Anim. Husb., 23: 619-626, 2007.
24. A.S. Bayar, S. Bilici, E.İ. Cengiz, A. Satar, M. Yanar, “The effects of Vitamin E supplemantation on ovary and testis histopathology in Oreochromis niloticus exposed to deltamethrin”, Toxicological & Environmental Chemistry, 96:1, 114-135, 2014.
25. R.E. Madureira, C. Cruzeiro, C., M.J. Rocha, E. Rocha, “The toxicity potential of pharmaceuticals found in the Douro River estuary (Portugal)—Experimental assessment using a zebrafish embryo test”, Environmental Toxicology and Pharmacology, 32(2): 212-217, 2011.
26. N. Ağırbaşlı, “Çevresel Kirleticileri Etofenpoksun Zebra Balığı (Danio rerio)‘da Genotoksik ve Histopatolojik Etkilerinin Belirlenmesi”, Gazi Üniversitesi. Doktora tezi. 2016.
27. A.M. Vibhandik, R.B. Gaikwad, G.P. Wani, “Fenvalerate Induced Changes in Histological Structure of Gonads of Freshwater Teleost Fish Barbus carnaticus”, International Journal of Scientific Research in Science and Technology, 6(2): 138-142, 2019.
28. H. Chen, K. Chen, X. Qiu, H. Xu, G. Mao, T. Zhao, W. Feng, E.S. Okeke, X. Wu, L. Yang, “The reproductive toxicity and potential mechanisms of combined exposure to dibutyl phthalate and diisobutyl phthalate in male zebrafish (Danio rerio)”, Chemosphere, 258: 127238, 2020.
29. K. Maharajan, S. Muthulakshmi, C. Karthik, B. Nataraj, K. Nambirajan, D. Hemalatha, S. Jiji, K. Kadirvelu, K. Liu, M. Ramesh, “Pyriproxyfen induced impairment of reproductive endocrine homeostasis and gonadal histopathology in zebrafish (Danio rerio) by altered expression of hypothalamus-pituitary-gonadal (HPG) axis genes” Science of The Total Environment, 735: 139496, 2020.
30. V.S. Oliveria, A.J.G. Castro, K. Marinsi, K.B. Mendes, G.A.A. Leite, A. Zamoner, G.D. Kraak, F.R.M.B. Silva, “Pyriproxyfen induces intracellular calcium overload and alters antioxidant defenses in Danio rerio testis that may influence ongoing spermatogenesis”, Environmental Pollution, 270: 116055, 2021.
31. J. Jiang, L. Chen, X. Liu, L. Wang, S. Wu, X. Zhao, “Histology and multi-omic profiling reveal the mixture toxicity of tebuconazole and difenoconazole in adult zebrafish”, Science of The Total Environment, 795(15): 148777, 2021.