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Centaurea balsamita Lam. ve Centaurea coronopifolia Lam. Türlerine Ait Farklı Ekstraktların Antimikrobiyal Aktivitesi

Yıl 2022, Cilt 2, Sayı 2, 107 - 114, 30.09.2022

Öz

Centaurea türleri Türk geleneksel tıbbında çeşitli hastalıkların tedavisinde yaygın olarak kullanılmaktadır. Bu çalışmada, Centaurea balsamita Lam. and Centaurea coronopifolia Lam.türlerinin kloroform, etil asetat, aseton ve etanol ekstraktlarının çeşitli bakterilere karşı (Bacillus cereus ATCC 14579, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Escherichia coli ATCC 29988, Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 6538, Salmonella typhimurium ATCC 14028, Streptococcus salivarius RSHE 606) antimikrobiyal aktiviteleri broth mikrodilüsyon metodu kullanılarak araştırılmıştır. Sonuçlar, çalışmada test edilen mikroorganizmalara karşı bitki ekstraktlarının farklı ölçülerde antimikrobiyal aktiviteye sahip olduğunu göstermiştir. En yüksek antimikrobiyal aktiviteyi C. balsamita’nın kloroform ekstresi B. cereus’a karşı göstermiştir (0,039 µg/mL). Ayrıca, etanol ekstraktı dışında tüm ekstraktlar S. aureus suşlarına karşı yüksek antimikrobiyal aktivite göstermiştir (20 µg/mL).

Kaynakça

  • [1] D. A. Dias, S. Urban, and U. Roessner, “A historical overview of natural products in drug discovery,” Metabolites, vol. 2, no. 2, pp. 303–336, Apr. 2012.
  • [2] P. Thomson, J. Jones, M. Browne, and S. J. Leslie, “Why people seek complementary and alternative medicine before conventional medical treatment: A population based study,” Complement. Ther. Clin. Pract., vol. 20, no. 4, pp. 339–346, Nov. 2014.
  • [3] N. Bachtel and K. Israni-winger, “Plant-based Medicine and Pharmacology,” vol. 93, pp. 227–228, 2020.
  • [4] S. Mickymaray, “antibiotics Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens.”
  • [5] Y. Tekeli, G. Zengin, A. Aktumsek, S. Mehmet, and E. Torlak, “Antibacterial activities of extracts from twelve Centaurea species from Turkey,” Arch. Biol. Sci., vol. 63, no. 3, pp. 685–690, 2011.
  • [6] N. Sharonova et al., “Comparative assessment of the phytochemical composition and biological activity of extracts of flowering plants of centaurea cyanus l., centaurea jacea l., and centaurea scabiosa l.,” Plants, vol. 10, no. 7, pp. 1–19, 2021.
  • [7] Amina Khammar and S. Djeddi, “Pharmacological and Biological Properties of some Centaurea Species,” Eur. J. Sci. Res., vol. 3, no. 84, pp. 398–416, 2012.
  • [8] B. Mathew, P. H. Davis, R. R. Mill, and K. Tan, “Flora of Turkey. Volume 10,” Kew Bull., vol. 45, no. 2, pp. 489–501, 1990.
  • [9] A. (Adil) Güner, N. Özhatay, T. Ekim, K. H. C. (Kemal H. C. Başer, and I. C. (Ian C. Hedge, “Flora of Turkey and the East Aegean Islands. Volume 11, Supplement 2,” vol. 11, p. 163, 2000.
  • [10] A. Ertas, A. C. Gören, M. Boga, S. Demirci, and U. Kolak, “Chemical Composition of The Essential Oils of Three Centaurea Species Growing Wild in Anatolia and Their Anticholinesterase Activities,” J. Essent. Oil-Bearing Plants, vol. 17, no. 5, pp. 922–926, 2014.
  • [11] K. Güven, S. Çelik, and I. Uysal, “Antimicrobial activity of Centaurea species,” Pharm. Biol., vol. 43, no. 1, pp. 67–71, 2005.
  • [12] M. Kargıoğlu, S. Cenkci, A. Serteser, M. Konuk, and G. Vural, “Traditional Uses of Wild Plants in the Middle Aegean Region of Turkey.”
  • [13] M. Alper and H. Güneş, “The anticancer and anti-inflammatory effects of Centaurea solstitialis extract on human cancer cell lines,” Turkish J. Pharm. Sci., vol. 16, no. 3, pp. 273–281, 2019.
  • [14] C. Karamenderes, S. Khan, B. L. Tekwani, M. R. Jacob, and I. A. Khan, “Antiprotozoal and Antimicrobial Activities of Centaurea. Species Growing in Turkey,” http://dx.doi.org/10.1080/13880200600883080, vol. 44, no. 7, pp. 534–539, Sep. 2008.
  • [15] E. H. Reda et al., “Comparative Study on the Essential Oils from Five Wild Egyptian Centaurea Species: Effective Extraction Techniques, Antimicrobial Activity and In-Silico Analyses,” Antibiot. 2021, Vol. 10, Page 252, vol. 10, no. 3, p. 252, Mar. 2021.
  • [16] R. Arif, E. Küpeli, F. Ergun, G. Üniversitesi, and E. Fakültesi, “THE BIOLOGICAL ACTIVITY OF CENTAUREA L. SPECIES (Review),” Fen Bilim. Derg., vol. 17, no. 4, pp. 149–164, 2004.
  • [17] N. Vaou, E. Stavropoulou, C. Voidarou, C. Tsigalou, and E. Bezirtzoglou, “microorganisms Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives,” 2021.
  • [18] WHO, “Antimicrobial resistance.” [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance. [Accessed: 28-Jul-2022].
  • [19] A. Ugur, N. Sarac, O. Ceylan, and M. Emin Duru, “Antimicrobial activity and chemical composition of endemic Centaurea cariensis subsp. niveo-tomentosa,” Nat. Prod. Res., vol. 24, no. 9, pp. 861–872, 2010.
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  • [22] N. Vaou, E. Stavropoulou, C. Voidarou, C. Tsigalou, and E. Bezirtzoglou, “Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives,” Microorg. 2021, Vol. 9, Page 2041, vol. 9, no. 10, p. 2041, Sep. 2021.
  • [23] A. Duran, H. Duman, A. Cansaran, N. Mercan Doan, M. Öztekin, and G. Acar, “Antimicrobial activity of various extracts of Centaurea cankiriense,” African J. Microbiol. Res., vol. 4, no. 8, pp. 608–612, 2010.
  • [24] J. N. Eloff, “Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results,” BMC Complement. Altern. Med., vol. 19, no. 1, pp. 1–8, 2019.
  • [25] N. J. Hilliard, R. L. Schelonka, and K. B. Waites, “Bacillus cereus Bacteremia in a Preterm Neonate,” J. Clin. Microbiol., vol. 41, no. 7, p. 3441, Jul. 2003.
  • [26] J. L. M. D. Gaur, Aditya H. M.D.; Shenep, “THE EXPANDING SPECTRUM OF DISEASES CAUSED BY BACILLUS CEREUS,” Pediatr. Infect. Dis. J., vol. 20, no. 5, pp. 533–534, 2001.
  • [27] E. J. G. Pollitt, P. T. Szkuta, N. Burns, and S. J. Foster, “Staphylococcus aureus infection dynamics,” PLOS Pathog., vol. 14, no. 6, p. e1007112, Jun. 2018.
  • [28] Taylor TA and Unakal CG, “Staphylococcus Aureus - StatPearls - NCBI Bookshelf,” StatPearls Publishing, 2022. [Online]. Available: https://www.ncbi.nlm.nih.gov/books/NBK441868/. [Accessed: 10-Aug-2022].
  • [29] F. D. Lowy, “Antimicrobial resistance: the example of Staphylococcus aureus,” J. Clin. Invest., vol. 111, no. 9, p. 1265, May 2003.
  • [30] M. Boğa et al., “Phytochemical profile and some biological activities of three centaurea species from Turkey,” Trop. J. Pharm. Res., vol. 15, no. 9, pp. 1865–1875, 2016.
  • [31] A. Trendafilova and M. Todorova, “Comparison of different techniques for extraction of biologically active compounds from Achillea millefolium Proa,” Nat. Prod. Commun., vol. 3, no. 9, pp. 1515–1518, 2008.
  • [32] A. Ugur, N. Sarac, O. Ceylan, and M. Duru, “Chemical composition of endemic Centaurea austro-anatolica and studies of its antimicrobial activity against multi-resistant bacteria,” Acta Pharm., vol. 59, no. 4, pp. 463–472, Dec. 2009.
  • [33] P. V. Licciardi and J. R. Underwood, “Plant-derived medicines: A novel class of immunological adjuvants,” Int. Immunopharmacol., vol. 11, no. 3, pp. 390–398, Mar. 2011.

Antimicrobial Activity of Various Extracts of Centaurea balsamita Lam. And Centaurea coronopifolia Lam.

Yıl 2022, Cilt 2, Sayı 2, 107 - 114, 30.09.2022

Öz

Members of Centaurea have been widely used to treat various diseases in Turkish folk medicine. In this research, the antimicrobial activities of the chloroform, ethyl acetate, acetone, and ethanol extracts from Centaurea balsamita Lam. and Centaurea coronopifolia Lam. were investigated against some bacteria (Bacillus cereus ATCC 14579, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Escherichia coli ATCC 29988, Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 6538, Salmonella typhimurium ATCC 14028, Streptococcus salivarius RSHE 606) by microdilutation method. The results showed that the extracts have antimicrobial activity of different range against the test microorganisms used in this study. The chloroform extract of C. balsamita showed the highest antimicrobial activity against B. cereus strain (0,039 µg/mL). Also, all the extracts used in this study have significant antimicrobial activity against S. aureus strains (20 µg/mL), except the ethanol extracts which had lower activity.

Kaynakça

  • [1] D. A. Dias, S. Urban, and U. Roessner, “A historical overview of natural products in drug discovery,” Metabolites, vol. 2, no. 2, pp. 303–336, Apr. 2012.
  • [2] P. Thomson, J. Jones, M. Browne, and S. J. Leslie, “Why people seek complementary and alternative medicine before conventional medical treatment: A population based study,” Complement. Ther. Clin. Pract., vol. 20, no. 4, pp. 339–346, Nov. 2014.
  • [3] N. Bachtel and K. Israni-winger, “Plant-based Medicine and Pharmacology,” vol. 93, pp. 227–228, 2020.
  • [4] S. Mickymaray, “antibiotics Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens.”
  • [5] Y. Tekeli, G. Zengin, A. Aktumsek, S. Mehmet, and E. Torlak, “Antibacterial activities of extracts from twelve Centaurea species from Turkey,” Arch. Biol. Sci., vol. 63, no. 3, pp. 685–690, 2011.
  • [6] N. Sharonova et al., “Comparative assessment of the phytochemical composition and biological activity of extracts of flowering plants of centaurea cyanus l., centaurea jacea l., and centaurea scabiosa l.,” Plants, vol. 10, no. 7, pp. 1–19, 2021.
  • [7] Amina Khammar and S. Djeddi, “Pharmacological and Biological Properties of some Centaurea Species,” Eur. J. Sci. Res., vol. 3, no. 84, pp. 398–416, 2012.
  • [8] B. Mathew, P. H. Davis, R. R. Mill, and K. Tan, “Flora of Turkey. Volume 10,” Kew Bull., vol. 45, no. 2, pp. 489–501, 1990.
  • [9] A. (Adil) Güner, N. Özhatay, T. Ekim, K. H. C. (Kemal H. C. Başer, and I. C. (Ian C. Hedge, “Flora of Turkey and the East Aegean Islands. Volume 11, Supplement 2,” vol. 11, p. 163, 2000.
  • [10] A. Ertas, A. C. Gören, M. Boga, S. Demirci, and U. Kolak, “Chemical Composition of The Essential Oils of Three Centaurea Species Growing Wild in Anatolia and Their Anticholinesterase Activities,” J. Essent. Oil-Bearing Plants, vol. 17, no. 5, pp. 922–926, 2014.
  • [11] K. Güven, S. Çelik, and I. Uysal, “Antimicrobial activity of Centaurea species,” Pharm. Biol., vol. 43, no. 1, pp. 67–71, 2005.
  • [12] M. Kargıoğlu, S. Cenkci, A. Serteser, M. Konuk, and G. Vural, “Traditional Uses of Wild Plants in the Middle Aegean Region of Turkey.”
  • [13] M. Alper and H. Güneş, “The anticancer and anti-inflammatory effects of Centaurea solstitialis extract on human cancer cell lines,” Turkish J. Pharm. Sci., vol. 16, no. 3, pp. 273–281, 2019.
  • [14] C. Karamenderes, S. Khan, B. L. Tekwani, M. R. Jacob, and I. A. Khan, “Antiprotozoal and Antimicrobial Activities of Centaurea. Species Growing in Turkey,” http://dx.doi.org/10.1080/13880200600883080, vol. 44, no. 7, pp. 534–539, Sep. 2008.
  • [15] E. H. Reda et al., “Comparative Study on the Essential Oils from Five Wild Egyptian Centaurea Species: Effective Extraction Techniques, Antimicrobial Activity and In-Silico Analyses,” Antibiot. 2021, Vol. 10, Page 252, vol. 10, no. 3, p. 252, Mar. 2021.
  • [16] R. Arif, E. Küpeli, F. Ergun, G. Üniversitesi, and E. Fakültesi, “THE BIOLOGICAL ACTIVITY OF CENTAUREA L. SPECIES (Review),” Fen Bilim. Derg., vol. 17, no. 4, pp. 149–164, 2004.
  • [17] N. Vaou, E. Stavropoulou, C. Voidarou, C. Tsigalou, and E. Bezirtzoglou, “microorganisms Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives,” 2021.
  • [18] WHO, “Antimicrobial resistance.” [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance. [Accessed: 28-Jul-2022].
  • [19] A. Ugur, N. Sarac, O. Ceylan, and M. Emin Duru, “Antimicrobial activity and chemical composition of endemic Centaurea cariensis subsp. niveo-tomentosa,” Nat. Prod. Res., vol. 24, no. 9, pp. 861–872, 2010.
  • [20] Clinical and Laboratory Standards Institute, “Clinical and Laboratory Standards Institute,” Wane, Pennsylvania: Clinical and Laboratory Satandards Institute, 2019, pp. 604–604.
  • [21] C. Valgas, S. M. De Souza, E. F. A. Smânia, and A. Smânia, “Screening methods to determine antibacterial activity of natural products,” Brazilian J. Microbiol., vol. 38, no. 2, pp. 369–380, 2007.
  • [22] N. Vaou, E. Stavropoulou, C. Voidarou, C. Tsigalou, and E. Bezirtzoglou, “Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives,” Microorg. 2021, Vol. 9, Page 2041, vol. 9, no. 10, p. 2041, Sep. 2021.
  • [23] A. Duran, H. Duman, A. Cansaran, N. Mercan Doan, M. Öztekin, and G. Acar, “Antimicrobial activity of various extracts of Centaurea cankiriense,” African J. Microbiol. Res., vol. 4, no. 8, pp. 608–612, 2010.
  • [24] J. N. Eloff, “Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results,” BMC Complement. Altern. Med., vol. 19, no. 1, pp. 1–8, 2019.
  • [25] N. J. Hilliard, R. L. Schelonka, and K. B. Waites, “Bacillus cereus Bacteremia in a Preterm Neonate,” J. Clin. Microbiol., vol. 41, no. 7, p. 3441, Jul. 2003.
  • [26] J. L. M. D. Gaur, Aditya H. M.D.; Shenep, “THE EXPANDING SPECTRUM OF DISEASES CAUSED BY BACILLUS CEREUS,” Pediatr. Infect. Dis. J., vol. 20, no. 5, pp. 533–534, 2001.
  • [27] E. J. G. Pollitt, P. T. Szkuta, N. Burns, and S. J. Foster, “Staphylococcus aureus infection dynamics,” PLOS Pathog., vol. 14, no. 6, p. e1007112, Jun. 2018.
  • [28] Taylor TA and Unakal CG, “Staphylococcus Aureus - StatPearls - NCBI Bookshelf,” StatPearls Publishing, 2022. [Online]. Available: https://www.ncbi.nlm.nih.gov/books/NBK441868/. [Accessed: 10-Aug-2022].
  • [29] F. D. Lowy, “Antimicrobial resistance: the example of Staphylococcus aureus,” J. Clin. Invest., vol. 111, no. 9, p. 1265, May 2003.
  • [30] M. Boğa et al., “Phytochemical profile and some biological activities of three centaurea species from Turkey,” Trop. J. Pharm. Res., vol. 15, no. 9, pp. 1865–1875, 2016.
  • [31] A. Trendafilova and M. Todorova, “Comparison of different techniques for extraction of biologically active compounds from Achillea millefolium Proa,” Nat. Prod. Commun., vol. 3, no. 9, pp. 1515–1518, 2008.
  • [32] A. Ugur, N. Sarac, O. Ceylan, and M. Duru, “Chemical composition of endemic Centaurea austro-anatolica and studies of its antimicrobial activity against multi-resistant bacteria,” Acta Pharm., vol. 59, no. 4, pp. 463–472, Dec. 2009.
  • [33] P. V. Licciardi and J. R. Underwood, “Plant-derived medicines: A novel class of immunological adjuvants,” Int. Immunopharmacol., vol. 11, no. 3, pp. 390–398, Mar. 2011.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji
Bölüm Araştırma Makaleleri
Yazarlar

Nuray YILDIRIM> (Sorumlu Yazar)
Çanakkale Onsekiz Mart Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu
0000-0002-4807-5357
Türkiye


Mehtap AKIN>
SELÇUK ÜNİVERSİTESİ, FEN FAKÜLTESİ
0000-0003-2554-236X
Türkiye


Hatice TANER SARAÇOĞLU>
SELÇUK ÜNİVERSİTESİ, FEN FAKÜLTESİ
0000-0001-9502-3739
Türkiye

Teşekkür The authors thank Dr. Osman Tugay and Dr. Tuna Uysal from Selçuk University for their help in identifying the plants.
Yayımlanma Tarihi 30 Eylül 2022
Yayınlandığı Sayı Yıl 2022, Cilt 2, Sayı 2

Kaynak Göster

IEEE N. Yıldırım , M. Akın ve H. Taner Saraçoğlu , "Antimicrobial Activity of Various Extracts of Centaurea balsamita Lam. And Centaurea coronopifolia Lam.", Environmental Toxicology and Ecology, c. 2, sayı. 2, ss. 107-114, Eyl. 2022