The Influence of Dietary Boron Supplementation on Serum Calcium, Inorganic Phosphorus, Magnesium Levels and Alkaline Phosphatase Activity in Quails

Yıl 2006, Cilt: 15 Sayı: 1, 8 - 14, 01.03.2006

Nurkan Karabulut Meryem Eren

Öz

This study was conducted to investigate the effects of dietary boron (B) supplementation on serum calcium (Ca), inorganic phosphorus (Pi), magnesium (Mg) levels and alkaline phosphatase (ALP) activity related to bone metabolism in quails. Two hundred and eighty, Japanese quails (Coturnix coturnix Japonica) aged one-day were divided into five groups with four replicates and they were fed 0, 10, 60, 120 or 240 mg/kg B supplemented diets until 35 days of age. At the end of the study, a total of 20 blood samples were collected from each group and serum Ca, Pi, Mg levels and ALP activity were determined. Serum Ca levels decreased in all groups, Pi levels decreased in 120 and 240 mg/kg B supplemented groups and also Mg levels decreased 60, 120 or 240 mg/kg B supplemented groups. No changes were observed in serum ALP activity. In conclusion, 10 mg/kg and over doses of B supplementation to quail diet except ALP activity, decreased serum Ca, Pi and Mg levels, indicators of mineral and bone metabolism of organism. Therefore, further studies with lower levels of B in the form of boric acid supplementation may be more valuable for the field applications

Anahtar Kelimeler

Alkaline phosphatase, quail, boron, mineral

BESİ BILDIRCINI YEMLERİNE BOR İLAVESİNİN SERUM KALSİYUM, İNORGANİK FOSFOR VE MAGNEZYUM DÜZEYLERİ İLE ALKALİ FOSFATAZ AKTİVİTESİNE ETKİSİ

Öz

Bu çalışmada, besi bıldırcınlarında yeme ilave edilen bor (B)’un kemik metabolizması ile doğrudan ilişkili serum kalsiyum (Ca), inorganik fosfor (Pi) ve magnezyum (Mg) düzeyleri ile alkali fosfataz (ALP) aktivitesinde meydana getirebileceği değişimler araştırılmıştır. İkiyüz seksen adet günlük besi bıldırcını (Coturnix coturnix Japonica) dört tekrarlı beş gruba ayrılmış, 0, 10, 60, 120 ve 240 mg/kg düzeylerinde B ilave edilen yemlerle 35 günlük olana kadar beslenmiştir. Deneme sonunda her bir gruptan 20 hayvanın serumlarında Ca, Pi ve Mg düzeyleri ile ALP aktivitesi belirlenmiştir. Serum Ca düzeyleri tüm deneme gruplarında, Pi düzeyleri 120 ve 240 mg/kg ve Mg düzeyleri de 60, 120 ve 240 mg/kg B verilen gruplarda düşerken, serum ALP aktivitesinde herhangi bir değişim gözlenmemiştir. Sonuç olarak, organizmanın mineral ve kemik metabolizmasının göstergesi olan serum Ca, Pi ve Mg düzeyleri besi bıldırcını yemlerine 10 mg/kg ve üzeri düzeylerde B ilavesiyle düşmüştür, ALP aktivitesi ise etkilenmemiştir. Bu nedenle bıldırcın yemlerine 10 mg/kg ve daha düşük düzeylerde B ilavesinin yapıldığı çalışmaların yürütülmesinde yarar olacağı kanaatine varılmıştır

Anahtar Kelimeler

Alkali fosfataz, bıldırcın, bor, mineral

Kaynakça

• 1. WHO. Boron. International Programme on Chemical Safety. Environmental Health Criteria 204. Ohio, 1998, pp 1-201
• 2. Chapin RE, Ku WW, Kenney MA, et al. The effects of dietary boron on bone strength in rats. Fundam Applied Toxicol 1997, 35: 205- 215.
• 3. Hunt CD. Dietary boron modified the effects of magnesium and molybdenum on mineral metabolism in the cholecalciferol-deficient chick. Biol Trace Elem Res 1989, 22: 201- 220
• 4. Eren M, Uyanık F, Küçükersan S. The influence of dietary boron supplementation on egg quality and serum calcium, inorganic phosphorus, magnesium levels and alkaline phosphatase activity in laying hens. Res Vet Sci 2004, 76: 203-210.
• 5. Beattie JH, Peace HS. The influence of a lowboron diet and boron supplementation on bone, major mineral and sex steroid metabolism in postmenopausal women. Br J Nutr 1993, 69:871-884.
• 6. Brown TF, McCormick ME, Morris DR, et al. Effects of dietary boron on mineral balance in sheep. Nutr Res 1989, 9:503-512.
• 7. Hoffman DJ, Sanderson CJ, Le Captain LJ, et al. Interactive effects of boron, selenium, and dietary protein on survival, growth, and physiology in mallard ducklings. Arch Environ Contam Toxicol 1991, 20:288-294.
• 8. Başoğlu A, Sevinç M, Güzelbektaş H, Civelek T. Short communication: Effect of borax on serum lipid profile in dogs. Online J Vet Res 2000, 4: 153-156.
• 9. Hall IH, Wong OT, Sood A, et al. Hypolipidaemic activity in rodents of boron analogs of phosphonoacetates and cyanoborane adducts of dialkyl aminomethylphosphonates. Pharmacol Res 1992, 25:259-270.
• 10. Hall IH, Spielvogel BF, Griffin TS, et al. The effects of boron hypolipidemic agents on LDL and HDL receptor binding and related enzyme activities of rat hepatocytes, aorta cells and human fibroblasts. Res Com Chem Path Pharm 1989, 65:297-317.
• 11. Hunt CD, Herbel JL. Boron affects energy metabolism in the streptozotocin-injected, vitamin D3-deprived rat. Magnes Trace Elem 1991-92, 10:374-386.
• 12. Hunt CD, Herbel JL. Effects of dietary boron on calcium and mineral metabolism in the streptozotocin-injected, vitamin D3-deprived rat. Magnes Trace Elem 1991-92,10:387-408.
• 13. Armstrong TA, Spears JW. Effect of boron supplementation of pig diets on the production of tumor necrosis factor-α and interferon-γ. J Anim Sci 2003, 81:2552-2561.
• 14. Armstrong TA, Spears JW, Lloyd KE. Inflammatory response, growth, and thyroid hormone concentrations are affected by longterm boron supplementation in gilts. J Anim Sci 2001, 79: 1549-1556.
• 15. Gallardo-Williams MT, Maronpot RR, et al. Effects of boric acid supplementation on bone histomorphometry metabolism, and biomechanical properties in aged female F-344 rats. Biol Trace Elem Res 2003, 93:155-170.
• 16. Penland JG. Dietary boron, brain function, and cognitive performance. Environ Health Perspect 1994, 102:65-72
• 17. Penland JG. The importance of boron nutrition for brain and psychological function. Biol Trace Elem Res 1998, 66: 299-317 (Abstr).
• 18. Armstrong TA, Spears JW, Crenshaw TD, et al. Boron supplementation of a semipurified diet for weanling pigs improves feed efficiency and bone strength characteristics and alters plasma lipid metabolites. J Nutr 2000, 139: 2575-2581.
• 19. Armstrong TA, Spears JW. Effects of dietary boron on growth performance, calcium and phosphorus metabolism, and bone mechanical proprties in gowing barrows. J Anim Sci 2001, 79:3120-3127.
• 20. Kurtoğlu V, Kurtoğlu F, Coşkun B. Effects of boron supplementation of adequate and inadequate vitamin D3-containing diet on performance and serum biochemical characters of broiler chickens. Res Vet Sci 2001, 71: 183-187.
• 21. Hall IH, Chen SY, Rajendran KG, et al. Hypolipidemic, anti-obesity, antiinflammatory, anti-osteoporotic, and antineoplastic properties of amine carboxyboranes. Environ Health Perspect 1994, 102: 21-30.
• 22. Newnham RE. Essentially of boron for healthy bones and joints. Environ Health Perspect 1994, 102:83-85.
• 23. Nielsen FH, Schuler TR, Zimmerman TJ, et al. Dietary magnesium, manganese and boron affect the response of rats to high dietary aluminium. Magnes 1988, 7:133-147.
• 24. Dupre JN, Keenan MJ, Hegsted M, et al. Effects of dietary boron in rats fed a vitamin Ddeficient diet. Environ Health Perspect. 1994, 102: 55-58.
• 25. Hegsted M, Keenan MJ, Siver F, et al. Effect of boron on vitamin D deficient rats. Biol Trace Elem Res 1991, 28:243-255.
• 26. Elliot MA, Edwards HM. Studies to detemine whether an interaction exist among boron, calcium, and cholecalciferol on the skeletal development of broiler chickens. Poult Sci 1992, 71:677-690 (Abstr).
• 27. Kurtoğlu V, Kurtoğlu F, Coşkun B, ve ark. Effects of boron supplementation on performance and some serum biochemical parameters in laying hens. Revue Med Vet 2002, 153, 12: 823-828.
• 28. Underwood EJ, Suttle NF. The Mineral Nutrition of Livestock. 3rd ed. CABI Publishing, New York. 1999, 513-542.
• 29. National Academies. Instute of Medicine Press Release. January 9, 2001
• 30. Nielsen FH, Schuler TR. Studies of the interaction between boron and calcium, and its modification by magnesium and potassium in rats. Effects on growth, blood variables, and bone mineral composition. Biol Trace Elem Res 1992, 35: 225-237 (Abstr).
• 31. Nielsen FH, Hunt CD, Mu LM, et al. Effect of boron on mineral, estrogen, and testosterone metabolism in postmenopausal women. FASEB J 1987, 1: 394-397.
• 32. King N, Odom TW, Sampson HW, Pardeu S. In ovo administration of boron or sodium aluminosilicate alters mineralization in the turkey. Nutr Res 1993, 13:77-85.
• 33. Nielsen FH. Studies on the relationship between boron and magnesium which possibly affects the formation and maintanence of bones.Magnesium Trace Elem 1990,9:61-69.
• 34. Qin X, Klandorf H. Effect of dietary boron supplementation on egg production, shell quality, and calcium metabolism in aged broiler breeder hens. Poultry Sci 1991, 70: 2131- 2138.