BEYAZ KAS HASTALIKLI KUZULARDA KALP KASI HASARININ TEŞHİSİNDE KARDİYAK TROPONİN KİT ANALİZLERİ VE SERUM ENZİM AKTİVİTELERİNİN ÖNEMİ

Yıl 2008, Cilt: 17 Sayı: 3, 144 - 149, 01.12.2008

Bayram Sugen Vehbi Güneş

Öz

Bu çalışmada; kuzuların beyaz kas hastalığı
sonucunda oluşan myokard dejenerasyonlarının
teşhisinde, beşeri kardiyak troponin I (cTn-I) ve
kardiyak troponin T (cTn-T) ve serum enzim
aktivitelerinin önemlerinin araştırılması amaçlandı.
Bu amaçla 20 adet beyaz kas hastalıklı, 10 adet
sağlıklı kuzu kullanıldı. Kardiyak Tn-I ve cTn-T
testleri 5 beyaz kas hastalıklı kuzuda pozitif
belirlenirken, sağlıklı kuzuların tamamında negatif
sonuçlar elde edildi. Beyaz kas hastalıklı kuzularda
nekropsi bulgularında kalp ve iskelet kaslarının yer
yer tebeşir beyazı görünümünde olduğu belirlendi.
Yine bu kuzularda ortalama kreatin kinaz MB mass
(CK-MB), laktat dehidrogenaz (LDH) ve aspartat
aminotransferaz (AST) enzim serum aktiviteleri
sırasıyla 216,2±33,8 U/L, 717,7±657,5 U/L ve
126,4±83,3 U/L olarak bulundu. Aynı parametreler
sağlıklı kuzularda sırasıyla 171,2±60,6 U/L,
513,2±210,6 U/L ve 59,2±21,1 U/L idi. Ayrıca bu
parametreler cTn pozitif vakalarda (n=5)
490,5±167,7 U/L, 3804,1±2700,3 U/L ve 734.5±226.2
U/L idi. Bu grupta enzim aktiviteleri istatistiki olarak
daha yüksekti. Beyaz kas hastalıklı kuzularda
makroskobik bulgular, serum enzim aktiviteleri ve cTn
bulguları biribiri ile uyumluydu. Sonuçta küçük
ruminantların myokardiyal hasarlarının teşhisinde
cTn analiz sonuçlarının duyarlı ve özgün bir gösterge
olarak kullanılabileceği kanısına varıldı.

Anahtar Kelimeler

Beyaz kas hastalığı, kardiyak troponin-I, kardiyak troponin-T, kuzu

The Importance of Cardiac Troponin Kit Analyses and Serum Enzyme Activities in the Diagnosis of Heart Muscle Degeneration in Lambs with White Muscle Disease

Öz

The aim of this study was to investigate
the value of human cardiac troponin-I (cTn-I),
cardiac troponin-T (cTn-T) kits and serum enzymes
activities in the diagnosis of myocardial
degeneration in lambs with White Muscle Disease
(WMD). For this purpose, 20 lambs with acute WMD
and 10 clinically healthy lambs were used. Cardiac
Tn-I and cTn-T tests were determined as positive in
the only 5 lambs with WMD, but these tests were
negative in blood samples collected from healthy
lambs. In necropsy findings, the cardiac and skeletal
muscles were observed to be affected by chalky white
lesions. Mean Creatine Kinase-MB mass (CK-MB),
Lactate Dehydrogenase (LDH) and Aspartate
Aminotransferase (AST) enzyme activities were
found as 216,2±33,8 U/L, 717,7±657,5 U/L and
126,4±83,3 U/L respectively in lambs with WMD.
Same parameters were mean 171,2±60,6 U/L,
513,2±210,6 U/L and 59,2±21,1 U/L in healthy
lambs, respectively. Additionally these parameters
were determined as 490,5±167,7 U/L,
3804,1±2700,3 U/L and 734,5±226,2 U/L in cTn
positive cases (n=5) respectively. In this group mean
enzyme activities were statistically higher than in the
other groups. Macroscopic findings of heart muscle,
serum enzymes activities and positive cTn results
were correlated in cases with WMD. In conclusion,
cTn assays may be a sensitive and specific marker
for small ruminants with myocardial injury.

Anahtar Kelimeler

White muscle disease, cardiac troponinI, cardiac troponin-T, lamb

Kaynakça

• Aytuğ CN. Metabolizma ve Noksanlık Hastalıkları. Koyun-Keçi Hastalıkları ve Yetiştiriciliği. Teknografik Matbaası, İstanbul 1990, ss 289.
• Bilal T. Metabolizma ve eksiklik hastalıkları. Koyun-Keçilerin İç Hastalıkları ve Beslenmesi. İstanbul Üniversitesi, İstanbul 2005, ss 251– 257.
• Ergün A, Tuncer Ş. Hayvan Besleme ve Beslenme Hastalıkları. Medipres, Ankara 2001, ss 85– 91.
• Sarı M, Çerçi İH. Yemler. Hayvan Besleme ve Beslenme Hastalıkları. Elazığ 1993, ss 31–36.
• Ergün A. Hayvan Besleme ve Beslenme Hastalıkları. Medipres Matbaacılık Yayıncılık Ltd. Şti, Malatya 2004, ss 388–389.
• Azzazy HME, Christenson RH. Cardiac markers of acute coronary syndrome: Is there a case for point-of-care testing. Clin Biochem 2002, 35: 13 –27.
• O'brien PJ, Smith DE, Knechtel TJ, et al. Cardiac troponin I is a sensitive, specific biomarker of cardiac injury in laboratory animals. Lab Anim 2006, 40(2): 153-71.
• Gunes V, Atalan G, Citil M. Erdogan HM. Use of cardiac troponin kits for the qualitative determination of myocardial cell damage due to traumatic reticuloperitonitis in cattle. Vet Rec 2008, 19:514-7.
• M-Bardorff M, Hallermayer K, Schro A. Ebert C. Improved troponin T ELISA specific for cardiac troponin T isoform: assay development and analytical and clinical validation. Clin Chem 1999, 43(3): 458–466.
• Rice MS. Appropriate roles of cardiac troponins in evaluating patients with chest pain. J Am Board Fam Pract 1999, 12(3):214–218.
• Bertsch T, Bleuel H, Aufenanger J, et al. Comparison of cardiac troponin-T and cardiac troponin-I concentrations in peripheral blood during orciprenaline induced tachycardia in rats. Exp Toxicol Pathol 1997, 49: 467–468.
• Christenson RH, Apple FS, Morgan DL, et al. Cardiac troponin-I measurement with the Access immunoassay system: analytical and clinical performance characteristics. Clin Chem 1998, 44: 52–60.
• McAuliffe JJ, Robbins J. Troponin T expression in normal and pressure-loaded fetal sheep. Heart Pediatr Res 1991, 29: 580–585.
• Fredericks S, Merton GK, Lerena MJ, et al. Cardiac troponins and creatine kinase content of striated muscle in common laboratory animals. Clin Chim Acta 2001, 304: 65–74.
• Braun JP, Diquelou A, Trumel C. et al. Biochemical markers of cardiac damage and dysfunction in dogs and cats; 1st TSAVA “Anadolum” Continuing Education Congress, İstanbul, 10–11 November 2006, pp 23-27.
• Charles CJ, Elliott JM, Nicholls MG, Rademaker MT, Richards M. Myocardial infarction with and without reperfusion in sheep: early cardiac and neurohumoral changes. Clin Sci 2000, 98: 703–711.
• Tunca, R., Erdoğan, H.M., Sözmen, M., Çitil, M., Devrim, A. K., Erginsoy, S., Uzlu, E. Evaluation of Cardiac Troponin I and Inducible Nitric Oxide Synthase Expressions in Lambs with White Muscle Disease. Turkish Journal of Veterinary and Animal Science 2009 (Baskıda). http://journals.tubitak.gov.tr/ havuz/vet-0710-6.pdf. adresinden temin edilebilir.
• Gunes V, Erdogan HM, Citil M, Ozcan K. Assay of cardiac troponins in the diagnosis of myocardial degeneration due to foot-andmouth disease in a calf. Vet Rec 2005, 156: 714-715.
• Kokuuslu C, Drommer W, Özkul IA, Çamaş H. Kuzuların beyaz kas hastalığında oluşan histolojik ve elektron mikroskobik bulguların incelenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi 1986, 33: 97–112.