BibTex RIS Kaynak Göster

Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates

Yıl 2015, Cilt: 31 Sayı: 1, 16 - 19, 01.03.2015

Öz

Aim: In this study, effects of ultrasound homogenisation (Sonication) technique on the activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein in liver homogenates were investigated. Materials and Methods: Postmortem healthy fresh calf liver was used as the material. Liver was sliced and grouped as mechanical homogenisation (2 min) and sonication group (2, 4, 6, 8 and 10 second sonication). Activities of superoxide dismutase, glutathione peroxidase, catalase, levels of lipid peroxidation and total protein were measured in supernatant of homogenisated samples by spectrophotometric methods.Results: Superoxide dismutase, glutathione peroxidase, catalase activities and total protein levels in mechanical group were significantly different from sonication groups (P<0.05). In sonication groups, superoxide dismutase and glutathione peroxidase activities were higher and catalase activity was lower from mechanical group (P<0.05). As regards glutathione peroxidase activity, 8 sec sonication group was the lowest compared to 2, 4, 6 (P>0.05) and 10 sec (P<0.05) groups whereas 8 sec catalase activity was the highest compared to other sonication groups (P<0.05). Total protein level was the lowest in 8 sec group compared to the other sonication groups which significant difference was determined in 2, 6 and 10 sec (P<0.05) groups. Lipid peroxidation level was the highest in 8 sec sonication group compared to other sonication groups with a significance in 2 sec group (P<0.05). Conclusions: In liver homogenates, antioxidant enzyme activities, lipid peroxidation and total protein levels were significantly different between mechanical and ultrasound homogenisation groups. Sonication for 8 seconds suggested to be critical point.

Kaynakça

  • Akkuş İ, 1995. Serbest radikaller ve fizyopatolojik etkileri. Mimoza yayınları, Kuzucular Ofset, Konya, Türkiye, pp: 5-20.
  • Anonymous 2010. Ultrasonic Lysis: Cell Disruption & Extraction, http://www.hielscher.com/ ultrasonic-lysis-celldisruption-extraction.htm. Accessed at: 01.05.2014.
  • Bar R, 1988. Ultrasound enhanced bioprocesses, cholesterol oxidation by Rhodococcus erytropolis. Biotechnol Bioeng, 32, 655-663.
  • Burden DW, 2012. Guide to the disruption of biological samples. Random Primers, 12, 1-25.
  • Edmonds PD, Sancier KM, 1983. Evidence for free radical production by ultrasonic cavitation in biological media. Ultrasound Med Biol, 9, 635-639.
  • Erte E, 2007. Siyah üzümde (Vitis vinifera L.) bulunan resveratrolün üretim veriminin artırılmasına ses ötesi dalgaların etkisi. Yüksek lisans tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Hawkins CL, Davies MJ, 2001. Generation and propagation of radical reactions on proteins. Biochim Biophys Acta, 1504, 196-219.
  • Kavutçu AD, 2006. Ultrason dalgaların fare böbrek dokusuna etkisi. Yüksek Lisans tezi, Gazi Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Mason TJ, Peters D, 2002. Practical sonochemistry: Uses and applications of ultrasound, 2nd edition, Horwood Publishing, Chichester, West Sussex, UK, pp: 113-151.
  • Milowska K,Gabryelak T, Lypacewicz G, Tymkiewicz R, Nowicki A, 2005. Effect of ultrasound on nucleated erythrocytes. Ultrasound Med Biol, 31, 129-134.
  • Satheeshkumar KS, Jayakumar R, 2002. Sonication induced sheet form-tion at the air - water interface. Chem Commun (Camb), 19, 2244-2245.
  • Stathopulos PB, Scholz GA, Hwang YM, Rumfeldt JAO, Lepock JR, Meiering EM, 2001. Sonication of proteins causes formation of aggregates that resemble amyloid. Protein Sci, 13, 3017-3027.
  • Takatsuki H, Liu Y, Yoshikoshi A, Wang B, Sakanishi A, 2003. Effects of ultrasound on the growth and vakoular H+- ATPase activity of Aleo Callus Cells. Colloids Surf B, 32, 105-116.
  • Wood RW, Loomis AL, 1927. The physical and biological effects of high frequency sound waves of great intensity. Philos Mag, 4, 414-436.

Ultrasound homojenizasyonun karaciğer homojenatlarında süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ve lipid peroksit düzeylerine etkileri

Yıl 2015, Cilt: 31 Sayı: 1, 16 - 19, 01.03.2015

Öz

Amaç: Çalışmada mekanik ve ultrasound (sonikasyon) homojenizasyon tekniklerinin karaciğer süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ile lipid peroksitleri ve total protein düzeylerine etkileri araştırılmıştır. Gereç ve Yöntem: Bu amaçla taze dana karaciğeri küçük parçalara ayrılarak mekanik homojenizasyon (2 dk) ve sonikasyon (2, 4, 6, 8 ve 10 sn) grupları oluşturuldu. Süperoksit dismutaz, glutasyon peroksidaz, katalaz enzim aktiviteleri ile lipid peroksitleri ve total protein düzeyleri homojenatların süpernatantında spektrofotometrik yöntemlerle belirlendi.Bulgular: Süperoksit dismutaz, glutasyon peroksidaz, katalaz aktiviteleri ile total protein düzeylerinin, mekanik homojenizasyon gruplarında, sonikasyon grubuna göre önemli düzeyde farklı (P

Kaynakça

  • Akkuş İ, 1995. Serbest radikaller ve fizyopatolojik etkileri. Mimoza yayınları, Kuzucular Ofset, Konya, Türkiye, pp: 5-20.
  • Anonymous 2010. Ultrasonic Lysis: Cell Disruption & Extraction, http://www.hielscher.com/ ultrasonic-lysis-celldisruption-extraction.htm. Accessed at: 01.05.2014.
  • Bar R, 1988. Ultrasound enhanced bioprocesses, cholesterol oxidation by Rhodococcus erytropolis. Biotechnol Bioeng, 32, 655-663.
  • Burden DW, 2012. Guide to the disruption of biological samples. Random Primers, 12, 1-25.
  • Edmonds PD, Sancier KM, 1983. Evidence for free radical production by ultrasonic cavitation in biological media. Ultrasound Med Biol, 9, 635-639.
  • Erte E, 2007. Siyah üzümde (Vitis vinifera L.) bulunan resveratrolün üretim veriminin artırılmasına ses ötesi dalgaların etkisi. Yüksek lisans tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Hawkins CL, Davies MJ, 2001. Generation and propagation of radical reactions on proteins. Biochim Biophys Acta, 1504, 196-219.
  • Kavutçu AD, 2006. Ultrason dalgaların fare böbrek dokusuna etkisi. Yüksek Lisans tezi, Gazi Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
  • Mason TJ, Peters D, 2002. Practical sonochemistry: Uses and applications of ultrasound, 2nd edition, Horwood Publishing, Chichester, West Sussex, UK, pp: 113-151.
  • Milowska K,Gabryelak T, Lypacewicz G, Tymkiewicz R, Nowicki A, 2005. Effect of ultrasound on nucleated erythrocytes. Ultrasound Med Biol, 31, 129-134.
  • Satheeshkumar KS, Jayakumar R, 2002. Sonication induced sheet form-tion at the air - water interface. Chem Commun (Camb), 19, 2244-2245.
  • Stathopulos PB, Scholz GA, Hwang YM, Rumfeldt JAO, Lepock JR, Meiering EM, 2001. Sonication of proteins causes formation of aggregates that resemble amyloid. Protein Sci, 13, 3017-3027.
  • Takatsuki H, Liu Y, Yoshikoshi A, Wang B, Sakanishi A, 2003. Effects of ultrasound on the growth and vakoular H+- ATPase activity of Aleo Callus Cells. Colloids Surf B, 32, 105-116.
  • Wood RW, Loomis AL, 1927. The physical and biological effects of high frequency sound waves of great intensity. Philos Mag, 4, 414-436.
Toplam 14 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA59VZ27KD
Bölüm Araştırma
Yazarlar

Deniz S. Özdemir Bu kişi benim

Nuri Başpınar Bu kişi benim

Pınar P. Akalın Bu kişi benim

Yayımlanma Tarihi 1 Mart 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 31 Sayı: 1

Kaynak Göster

APA Özdemir, D. S., Başpınar, N., & Akalın, P. P. (2015). Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates. Eurasian Journal of Veterinary Sciences, 31(1), 16-19.
AMA Özdemir DS, Başpınar N, Akalın PP. Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates. Eurasian J Vet Sci. Mart 2015;31(1):16-19.
Chicago Özdemir, Deniz S., Nuri Başpınar, ve Pınar P. Akalın. “Effects of Ultrasound Homogenisation on the Activities of Superoxide Dismutase, Glutathione Peroxidase, Catalase and Levels of Lipid Peroxide in Liver Homogenates”. Eurasian Journal of Veterinary Sciences 31, sy. 1 (Mart 2015): 16-19.
EndNote Özdemir DS, Başpınar N, Akalın PP (01 Mart 2015) Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates. Eurasian Journal of Veterinary Sciences 31 1 16–19.
IEEE D. S. Özdemir, N. Başpınar, ve P. P. Akalın, “Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates”, Eurasian J Vet Sci, c. 31, sy. 1, ss. 16–19, 2015.
ISNAD Özdemir, Deniz S. vd. “Effects of Ultrasound Homogenisation on the Activities of Superoxide Dismutase, Glutathione Peroxidase, Catalase and Levels of Lipid Peroxide in Liver Homogenates”. Eurasian Journal of Veterinary Sciences 31/1 (Mart 2015), 16-19.
JAMA Özdemir DS, Başpınar N, Akalın PP. Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates. Eurasian J Vet Sci. 2015;31:16–19.
MLA Özdemir, Deniz S. vd. “Effects of Ultrasound Homogenisation on the Activities of Superoxide Dismutase, Glutathione Peroxidase, Catalase and Levels of Lipid Peroxide in Liver Homogenates”. Eurasian Journal of Veterinary Sciences, c. 31, sy. 1, 2015, ss. 16-19.
Vancouver Özdemir DS, Başpınar N, Akalın PP. Effects of ultrasound homogenisation on the activities of superoxide dismutase, glutathione peroxidase, catalase and levels of lipid peroxide in liver homogenates. Eurasian J Vet Sci. 2015;31(1):16-9.