BibTex RIS Kaynak Göster

First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines

Yıl 2014, Cilt: 30 Sayı: 1, 35 - 38, 01.03.2014

Öz

Coxiella species are known to be potentially pathogenic tickborne
organisms. This study was aimed to investigate the
presence of Coxiella sp. in Rhipicephalus sanguineus ticks in
Cebu, Philippines by molecular based techniques. A total of
164 ticks were collected from 36 dogs and analyzed by a Nested
Polymerase Chain Reaction (PCR) for the amplification of
partial 16S ribosomal RNA gene region. Nine ticks (5.5%) obtained
from 5 dogs were found positive. Sequencing revealed
partial DNA fragments of Coxiella spp. which were 96.3% and
98.1-100% identical to Coxiella burnetii and Coxiella sp. endosymbiont,
respectively. The detected DNA fragments also
shared 98.3-100% identities with each other. This study is
the first report on the existence of Coxiella spp. from Rhipicephalus
sanguineus ticks in Cebu, Philippines.

Kaynakça

  • Arricau-Bouvery N, Rodolakis A, 2005. Is Q fever an emerging or re-emerging zoonosis? Vet Res, 36, 327-349.
  • Beaufils JP, Inokuma H, Martin-Granel J, Jumelle P, BarbaultJumell M, Brouqui P, 2000. Anaplasma platys (Ehrlichia platys) infection in a dog in France: description of the case, and characterization of the agent. Revue Med Vet, 153, 85- 90.
  • Bermúdez CSE, Castro A, Esser H, Liefting Y, Garcia G, Miranda RJ, 2012. Ticks (Ixodida) on humans from central Panama, Panama (2010-2011). Exp Appl Acarol, 58, 81-88.
  • Bernasconi MV, Casati S, Peter O, Piffaretti JC, 2002. Rhipicephalus ticks infected with Rickettsia and Coxiella in Southern Switzerland (Canton Ticino). Infect Genet Evol, 2, 111-120.
  • Camer GA, Alejandria M, Amor M, Satoh H, Muramatsu Y, Ueno H, Morita C, 2003. Detection of antibodies against spotted fever group Rickettsia (SFGR), typhus group Rickettsia (TGR), and Coxiella burnetii in human febrile patients in the Philippines. Jpn J Infect Dis, 56, 26-28.
  • Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res, 32, 1792-1797.
  • Lang GH, 1990. Coxiellosis (Q fever) in animals, in: Q Fever. The Disease, ED: Marrie TJ, volume 1, CRC Press, USA, pp: 23-48.
  • Maurin M, Raoult D, 1999. Q fever. Clin Microbiol Rev, 12, 518-553.
  • Ta TH, Jiménez B, Navarro M, Meije Y, González FJ, LopezVelez R, 2008. Q Fever in returned febrile travelers. J Travel Med, 15, 126-129.
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 28, 2731-2739.
  • Tatsumi N, Baumgartner A, Qiao Y, Yamamoto I, Yamaguchi K, 2006. Detection of Coxiella burnetii in market chicken eggs and mayonnaise. Ann N Y Acad Sci, 1078, 502-505.
  • Walker JB, Keirans JE, Horak IG, 2000. The Genus Rhipicephalus (Acari, Ixodidae): A Guide to the Brown Ticks of the World, Cambridge University Press, USA.
  • Walker AR, Bouattour A, Camicas J-L, Estrada-Peña A, Horak IG, Latif AA, 2003. Ticks of domestic animals in Africa: A guide to identification of species, United Kingdom: Bioscience Reports.
  • Warner CK, Dawson JF, 1996. Genus- and species-level identification of Ehrlichia-species by PCR and sequencing. In: Persing DH, editor. PCR protocols for emerging infectious diseases: a supplement to Diagnostic Molecular Microbiology: principles and applications. American Society for Microbiology, Washington, USA, pp: 100-105.
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ, 1991. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol, 173, 697-703.
  • Weller SJ, Baldridge GD, Munderloh UG, Noda H, Simser J, Kurtti TJ, 1998. Phylogenetic placement of rickettsiae from the ticks Amblyomma americanum and Ixodes scapularis. J Clin Microbiol, 36, 1305-1317.
  • Williams KP, Gillespie JJ, Sobral BW, Nordberg EK, Snyder EE, Shallom JM, Dickerman AW, 2010. Phylogeny of gammaproteobacteria. J Bacteriol, 192, 2305-2314.
  • Ybañez AP, Perez ZO, Gabotero SR, Yandug RT, Kotaro M, Inokuma H, 2012. First molecular detection of Ehrlichia canis and Anaplasma platys in ticks from dogs in Cebu, Philippines. Ticks Tick Borne Dis, 3, 288-293.

Filipinler, Cebu’dan elde edilen Rhipicephalus sanguineus kenelerinden Coxiella spp. türlerinin moleküler olarak ilk belirlenmesi

Yıl 2014, Cilt: 30 Sayı: 1, 35 - 38, 01.03.2014

Öz

Coxiella türleri kene ısırması ile geçebilen patojenik etkendir.
Mevcut araştırma Filipinler, Cebu’da Rhipicephalus sanguineus
kenelerinden Coxielle türlerinin varlığını moleküler tekniklerle
belirlemek için yapıldı. Otuzaltı köpekten elde edilen
toplam 164 adet kene Nested Polimeraz Zincir Reaksiyonu
(PCR) ile kısmi 16S ribozomal RNA gen bölgesi çoğaltılarak
analiz edildi. Beş adet köpekten elde edilen 9 adet kene
pozitif (%5.5) bulundu. Sekans analizi sonucunda Coxiella
burnetii (%96.3) ve Coxiella sp. endosymbiont (%98.1-100)
ile benzer Coxiella spp.'ya ait DNA fragmentleri tespit edildi.
Belirlenen DNA fragmentleri birbirleri ile de %98.3-100
oranında benzerdi. Bu çalışma Filipinler Cebu'da Rhipicephalus
sanguineus kenelerinden elde edilen Coxielle spp. varlığı-
nı gösteren ilk araştırmadır.

Kaynakça

  • Arricau-Bouvery N, Rodolakis A, 2005. Is Q fever an emerging or re-emerging zoonosis? Vet Res, 36, 327-349.
  • Beaufils JP, Inokuma H, Martin-Granel J, Jumelle P, BarbaultJumell M, Brouqui P, 2000. Anaplasma platys (Ehrlichia platys) infection in a dog in France: description of the case, and characterization of the agent. Revue Med Vet, 153, 85- 90.
  • Bermúdez CSE, Castro A, Esser H, Liefting Y, Garcia G, Miranda RJ, 2012. Ticks (Ixodida) on humans from central Panama, Panama (2010-2011). Exp Appl Acarol, 58, 81-88.
  • Bernasconi MV, Casati S, Peter O, Piffaretti JC, 2002. Rhipicephalus ticks infected with Rickettsia and Coxiella in Southern Switzerland (Canton Ticino). Infect Genet Evol, 2, 111-120.
  • Camer GA, Alejandria M, Amor M, Satoh H, Muramatsu Y, Ueno H, Morita C, 2003. Detection of antibodies against spotted fever group Rickettsia (SFGR), typhus group Rickettsia (TGR), and Coxiella burnetii in human febrile patients in the Philippines. Jpn J Infect Dis, 56, 26-28.
  • Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res, 32, 1792-1797.
  • Lang GH, 1990. Coxiellosis (Q fever) in animals, in: Q Fever. The Disease, ED: Marrie TJ, volume 1, CRC Press, USA, pp: 23-48.
  • Maurin M, Raoult D, 1999. Q fever. Clin Microbiol Rev, 12, 518-553.
  • Ta TH, Jiménez B, Navarro M, Meije Y, González FJ, LopezVelez R, 2008. Q Fever in returned febrile travelers. J Travel Med, 15, 126-129.
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 28, 2731-2739.
  • Tatsumi N, Baumgartner A, Qiao Y, Yamamoto I, Yamaguchi K, 2006. Detection of Coxiella burnetii in market chicken eggs and mayonnaise. Ann N Y Acad Sci, 1078, 502-505.
  • Walker JB, Keirans JE, Horak IG, 2000. The Genus Rhipicephalus (Acari, Ixodidae): A Guide to the Brown Ticks of the World, Cambridge University Press, USA.
  • Walker AR, Bouattour A, Camicas J-L, Estrada-Peña A, Horak IG, Latif AA, 2003. Ticks of domestic animals in Africa: A guide to identification of species, United Kingdom: Bioscience Reports.
  • Warner CK, Dawson JF, 1996. Genus- and species-level identification of Ehrlichia-species by PCR and sequencing. In: Persing DH, editor. PCR protocols for emerging infectious diseases: a supplement to Diagnostic Molecular Microbiology: principles and applications. American Society for Microbiology, Washington, USA, pp: 100-105.
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ, 1991. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol, 173, 697-703.
  • Weller SJ, Baldridge GD, Munderloh UG, Noda H, Simser J, Kurtti TJ, 1998. Phylogenetic placement of rickettsiae from the ticks Amblyomma americanum and Ixodes scapularis. J Clin Microbiol, 36, 1305-1317.
  • Williams KP, Gillespie JJ, Sobral BW, Nordberg EK, Snyder EE, Shallom JM, Dickerman AW, 2010. Phylogeny of gammaproteobacteria. J Bacteriol, 192, 2305-2314.
  • Ybañez AP, Perez ZO, Gabotero SR, Yandug RT, Kotaro M, Inokuma H, 2012. First molecular detection of Ehrlichia canis and Anaplasma platys in ticks from dogs in Cebu, Philippines. Ticks Tick Borne Dis, 3, 288-293.
Toplam 18 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA64RC84NC
Bölüm Kısa Makale
Yazarlar

Adrian P. Ybañez Bu kişi benim

Yayımlanma Tarihi 1 Mart 2014
Yayımlandığı Sayı Yıl 2014 Cilt: 30 Sayı: 1

Kaynak Göster

APA Ybañez, A. P. (2014). First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines. Eurasian Journal of Veterinary Sciences, 30(1), 35-38.
AMA Ybañez AP. First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines. Eurasian J Vet Sci. Mart 2014;30(1):35-38.
Chicago Ybañez, Adrian P. “First Molecular Evidence of Coxiella Spp. From Rhipicephalus Sanguineus Ticks in Cebu, Philippines”. Eurasian Journal of Veterinary Sciences 30, sy. 1 (Mart 2014): 35-38.
EndNote Ybañez AP (01 Mart 2014) First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines. Eurasian Journal of Veterinary Sciences 30 1 35–38.
IEEE A. P. Ybañez, “First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines”, Eurasian J Vet Sci, c. 30, sy. 1, ss. 35–38, 2014.
ISNAD Ybañez, Adrian P. “First Molecular Evidence of Coxiella Spp. From Rhipicephalus Sanguineus Ticks in Cebu, Philippines”. Eurasian Journal of Veterinary Sciences 30/1 (Mart 2014), 35-38.
JAMA Ybañez AP. First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines. Eurasian J Vet Sci. 2014;30:35–38.
MLA Ybañez, Adrian P. “First Molecular Evidence of Coxiella Spp. From Rhipicephalus Sanguineus Ticks in Cebu, Philippines”. Eurasian Journal of Veterinary Sciences, c. 30, sy. 1, 2014, ss. 35-38.
Vancouver Ybañez AP. First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines. Eurasian J Vet Sci. 2014;30(1):35-8.