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Abort problemli sığırlarda Bovine Viral Diarrhea Virus enfeksiyonunun serolojik ve virolojik araştırılması

Yıl 2013, Cilt: 29 Sayı: 3, 159 - 162, 01.09.2013

Öz

Amaç: Bu çalışma Konya’da abort problemli bir sığırcılık işletmesinde Bovine Viral Diarrhea Virus (BVDV) enfeksiyonunun varlığının belirlenmesi amacı ile yapıldı. Gereç ve Yöntem: İnfertilite ve abort problemi görülen 228 sığırdan kan serumu ve lökosit örnekleri toplanarak BVDV antijen ve BVDV’ye karşı gelişen antikorlar yönünden Enzyme Linked Immunosorbent Assay ile incelendi.Bulgular: Araştırmada 41 (%17.9) serum örneği seropozitif, 4 (%1.7) lökosit örneği BVDV antijen pozitif olarak belirlendi. BVDV antijen pozitif bulunan 4 sığırın 2 (%0.8)’si seropozitif 2 (%0.8)’si ise seronegatif tespit edildi. Antijen pozitif/antikor negatif hayvanlar 2 hafta sonra tekrar örneklendi. Seronegatif sığırlar için aynı sonuçlar elde edildi. Persiste enfekte oldukları belirlenen bu hayvanlar kesime gönderildi. Öneri: İşletmelere alınacak olan hayvanların kontrol edilerek
hem BVDV antijen hem de antikor negatif olanların dahil edilmesi gerekmektedir.

Kaynakça

  • Baker JC, 1995. The clinical manifestations of bovine viral diarrhea infection. Vet Clin North Am Food Anim Pract, 11, 425-445.
  • Brinkhof J, Zimmer G, Westenbrink F, 1996. Comparative study on four enzyme-linked immunosorbent assays and a cocultivation assay for the detection of antigens associated with the bovine viral diarrhoea virus in persistently infected cattle. Vet Microbiol, 50, 1-6.
  • Collins ME, Desport M, Brownlie J, 1999. Bovine viral diarrhea virus quasispecies during persistent infection. Virology, 259, 85-98.
  • Corbett EM, Grooms DL, Bolin SR, Bartlett B, Grotelueschen DM, 2011. Use of sentinel serology in a bovine viral diarrhea virus eradication program. J Vet Diagn Invest, 23, 511515.
  • Cornish TE, van Olphen AL, Cavender JL, Edwards JM, Jaeger PT, Vieyra LL, Woodard LF, Miller DR, O’Toole D, 2005. Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus. J Vet Diagn Invest, 17, 110-117.
  • Justewicz DM, Magar R, Marsolais G, Lecomte J, 1987. Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurment of antibodies in bovine sera. Vet Immunol Immunopathol, 14, 377-384.
  • Handel IG, Willoughby K, Land F, Koterwas B, Morgan KL, Tanya VN, de Bronsvoort BM, 2011. Seroepidemiology of bovine viral diarrhoea virus (BVDV) in the Adamawa region of Cameroon and use of the SPOT test to identify herds with PI calves. Plos One, 6, e21620.
  • Hornberg A, Fernández SR, Vogl C, Vilcek S, Matt M, Fink M, Köfer J, Schöpf K, 2009. Genetic diversity of Pestivirus isolates in cattle from Western Austria. Vet Microbiol, 135, 205-213.
  • Houe H, 1999. Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV) infections. Vet Microbiol, 64, 89-107.
  • Howard CJ, Clarke MC, Brownlie J, 1985. An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine viral diarrhoea virus (BVDV) in cattle sera. Vet Microbiol, 10, 359-369.
  • Pacheco JM, Lager I, 2003. Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum. Rev Argent Microbiol, 35, 19-23.
  • Peterhans E, Schweizer M, 2010. Pestiviruses: How to outmaneuver your hosts. Vet Microbiol, 142, 18-25.
  • Safarpoor Dehkordi F, 2011. Prevalence study of bovine viral diarrhea virus by evaluation of antigen capture ELISA and RT-PCR assay in bovine, ovine, caprine, buffalo and camel aborted fetuses in Iran. AMB Express, 21, 1, 32.
  • Saliki JT, Huchzermeier R, Dubovi EJ, 2000. Evaluation of a new sandwich ELISA kit that uses serum for detection of cattle persistently infected with BVD virus. Ann N Y Acad Sci, 916, 358-363.
  • Sandvik T, 2005. Selection and use of laboratory diagnostic assays in BVD control programmes. Prev Vet Med, 15, 3-16.
  • Simsek A, Ozturk F, 1997. Investigation of persistent bovine viral diarrhea virus infection in clinically healty cattle herds and its epizootiologic importance. Eurasian J Vet Sci, 13, 113-119.
  • Tarry DW, Bernal L, Edwards S, 1991. Transmission of bovine virus diarrhoea virus by blood feeding flies. Vet Rec, 128, 82-84.
  • Xia H, Vijayaraghavan B, Belak S, Liu L, 2011. Detection and identification of the atypical Bovine Pestiviruses in commercial foetal bovine serum batches. Plos One, 6, 12, e28553.
  • Yapkic O, Bulut O, Simsek A, Yavru S, Kale M, Avci O, 2006a. Investigation of BHV-l, BVDV and EBLV infections in bulls selected for artificial insemination centers. Eurasian J Vet Sci, 22, 21-24.
  • Yapkic O, Yavru S, Bulut O, Kale M, Ata A, 2006b. Bovine viral diarrhoea Virus (BVDV) infection in pregnant cows and their foetuses. Bull Vet Inst Pulawy, 50, 315-317.
  • Zemke J, Knig P, Mischkale K, Reimann I, Beer M, 2010. Novel BVDV-2 mutants as new candidates for modified-live vaccines. Vet Microbiol, 142, 69-80.

Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem

Yıl 2013, Cilt: 29 Sayı: 3, 159 - 162, 01.09.2013

Öz

Aim: The aim of this study is to determine the presence of Bovine Viral Diarrhea Virus (BVDV) infection in a cattle herd with abortion problem in Konya.Materials and Methods: Totally 228 blood serum and 228 leukocytes taken from cattle selected according to criteria for infertile and abortion problems were examined for antigens and antibodies to BVDV by Enzyme Linked Immunosorbent Assay.Results: In this research, 41 (17.9%) sera were found seropositive and 4 (1.7%) leukocytes were BVDV antigen positive. Of these 4 BVDV antigen positive cattle, a number of 2 (0.8%) were detected seropositive while 2 (0.8%) were seronegative. The animals being antigen positive and antibody negative were sampled second time after two weeks. The same results were detected for two seronegative cattle. The animals detecting persistent infection status were sent to slaughter. Conclusion: It is recommended that the animals should be checked in terms of BVDV for being negative both antigen and antibody before accepting them to the herds.

Kaynakça

  • Baker JC, 1995. The clinical manifestations of bovine viral diarrhea infection. Vet Clin North Am Food Anim Pract, 11, 425-445.
  • Brinkhof J, Zimmer G, Westenbrink F, 1996. Comparative study on four enzyme-linked immunosorbent assays and a cocultivation assay for the detection of antigens associated with the bovine viral diarrhoea virus in persistently infected cattle. Vet Microbiol, 50, 1-6.
  • Collins ME, Desport M, Brownlie J, 1999. Bovine viral diarrhea virus quasispecies during persistent infection. Virology, 259, 85-98.
  • Corbett EM, Grooms DL, Bolin SR, Bartlett B, Grotelueschen DM, 2011. Use of sentinel serology in a bovine viral diarrhea virus eradication program. J Vet Diagn Invest, 23, 511515.
  • Cornish TE, van Olphen AL, Cavender JL, Edwards JM, Jaeger PT, Vieyra LL, Woodard LF, Miller DR, O’Toole D, 2005. Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus. J Vet Diagn Invest, 17, 110-117.
  • Justewicz DM, Magar R, Marsolais G, Lecomte J, 1987. Bovine viral diarrhea virus-infected MDBK monolayer as antigen in enzyme-linked immunosorbent assay (ELISA) for the measurment of antibodies in bovine sera. Vet Immunol Immunopathol, 14, 377-384.
  • Handel IG, Willoughby K, Land F, Koterwas B, Morgan KL, Tanya VN, de Bronsvoort BM, 2011. Seroepidemiology of bovine viral diarrhoea virus (BVDV) in the Adamawa region of Cameroon and use of the SPOT test to identify herds with PI calves. Plos One, 6, e21620.
  • Hornberg A, Fernández SR, Vogl C, Vilcek S, Matt M, Fink M, Köfer J, Schöpf K, 2009. Genetic diversity of Pestivirus isolates in cattle from Western Austria. Vet Microbiol, 135, 205-213.
  • Houe H, 1999. Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV) infections. Vet Microbiol, 64, 89-107.
  • Howard CJ, Clarke MC, Brownlie J, 1985. An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine viral diarrhoea virus (BVDV) in cattle sera. Vet Microbiol, 10, 359-369.
  • Pacheco JM, Lager I, 2003. Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum. Rev Argent Microbiol, 35, 19-23.
  • Peterhans E, Schweizer M, 2010. Pestiviruses: How to outmaneuver your hosts. Vet Microbiol, 142, 18-25.
  • Safarpoor Dehkordi F, 2011. Prevalence study of bovine viral diarrhea virus by evaluation of antigen capture ELISA and RT-PCR assay in bovine, ovine, caprine, buffalo and camel aborted fetuses in Iran. AMB Express, 21, 1, 32.
  • Saliki JT, Huchzermeier R, Dubovi EJ, 2000. Evaluation of a new sandwich ELISA kit that uses serum for detection of cattle persistently infected with BVD virus. Ann N Y Acad Sci, 916, 358-363.
  • Sandvik T, 2005. Selection and use of laboratory diagnostic assays in BVD control programmes. Prev Vet Med, 15, 3-16.
  • Simsek A, Ozturk F, 1997. Investigation of persistent bovine viral diarrhea virus infection in clinically healty cattle herds and its epizootiologic importance. Eurasian J Vet Sci, 13, 113-119.
  • Tarry DW, Bernal L, Edwards S, 1991. Transmission of bovine virus diarrhoea virus by blood feeding flies. Vet Rec, 128, 82-84.
  • Xia H, Vijayaraghavan B, Belak S, Liu L, 2011. Detection and identification of the atypical Bovine Pestiviruses in commercial foetal bovine serum batches. Plos One, 6, 12, e28553.
  • Yapkic O, Bulut O, Simsek A, Yavru S, Kale M, Avci O, 2006a. Investigation of BHV-l, BVDV and EBLV infections in bulls selected for artificial insemination centers. Eurasian J Vet Sci, 22, 21-24.
  • Yapkic O, Yavru S, Bulut O, Kale M, Ata A, 2006b. Bovine viral diarrhoea Virus (BVDV) infection in pregnant cows and their foetuses. Bull Vet Inst Pulawy, 50, 315-317.
  • Zemke J, Knig P, Mischkale K, Reimann I, Beer M, 2010. Novel BVDV-2 mutants as new candidates for modified-live vaccines. Vet Microbiol, 142, 69-80.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA69GD26PJ
Bölüm Araştırma
Yazarlar

Oya Bulut Bu kişi benim

Oguzhan Avci Bu kişi benim

Orhan Yapici Bu kişi benim

Sibel Yavru Bu kişi benim

Atilla Simsek Bu kişi benim

Yayımlanma Tarihi 1 Eylül 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 29 Sayı: 3

Kaynak Göster

APA Bulut, O., Avci, O., Yapici, O., Yavru, S., vd. (2013). Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem. Eurasian Journal of Veterinary Sciences, 29(3), 159-162.
AMA Bulut O, Avci O, Yapici O, Yavru S, Simsek A. Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem. Eurasian J Vet Sci. Eylül 2013;29(3):159-162.
Chicago Bulut, Oya, Oguzhan Avci, Orhan Yapici, Sibel Yavru, ve Atilla Simsek. “Serological and Virological Investigation of Bovine Viral Diarrhea Virus Infection in Cattle With Abortion Problem”. Eurasian Journal of Veterinary Sciences 29, sy. 3 (Eylül 2013): 159-62.
EndNote Bulut O, Avci O, Yapici O, Yavru S, Simsek A (01 Eylül 2013) Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem. Eurasian Journal of Veterinary Sciences 29 3 159–162.
IEEE O. Bulut, O. Avci, O. Yapici, S. Yavru, ve A. Simsek, “Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem”, Eurasian J Vet Sci, c. 29, sy. 3, ss. 159–162, 2013.
ISNAD Bulut, Oya vd. “Serological and Virological Investigation of Bovine Viral Diarrhea Virus Infection in Cattle With Abortion Problem”. Eurasian Journal of Veterinary Sciences 29/3 (Eylül 2013), 159-162.
JAMA Bulut O, Avci O, Yapici O, Yavru S, Simsek A. Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem. Eurasian J Vet Sci. 2013;29:159–162.
MLA Bulut, Oya vd. “Serological and Virological Investigation of Bovine Viral Diarrhea Virus Infection in Cattle With Abortion Problem”. Eurasian Journal of Veterinary Sciences, c. 29, sy. 3, 2013, ss. 159-62.
Vancouver Bulut O, Avci O, Yapici O, Yavru S, Simsek A. Serological and virological investigation of Bovine Viral Diarrhea Virus infection in cattle with abortion problem. Eurasian J Vet Sci. 2013;29(3):159-62.