DNA teknolojisi ile rendering ürünlerinde tür tespiti: Bir vaka raporu

Yıl 2012, Cilt: 28 Sayı: 2, 122 - 125, 01.06.2012

Ercan Kurar Yusuf Özşensoy Müge Doğan Bülbül Mehmet Nizamlıoğlu

Öz

Deli Dana Hastalığı (BSE) sonrası özellikle ruminant kökenli
rendering ürünlerinin hayvan rasyonlarında kullanılması
kontrol altına alınmıştır. Gümrük Beyan Belgesinde “hidrolize
tüy unu” olarak bildirilen yem materyalinden alınan örnek,
T.C. Gümrük Müsteşarlığı Mersin Gümrük Müdürlüğü
tarafından gönderilerek hayvansal kökeninin belirlenmesi
istenmiştir. İlgili örneğin mikroskobik incelenmesinde kanat
ve tüy parçalarının varlığı saptanmıştır. Alternatif olarak,
örneklerden standart fenol/kloroform ve dodecyltrimethylammonium
bromide (DTAB) yöntemleri ile DNA izolasyonu
yapılmıştır. Elde edilen DNA örnekleri kanatlı, ruminant,
domuz, at ve karnivor spesifik primerler kullanılarak
Polimeraz Zincir Reaksiyonu (PZR) ile yükseltgenmiş-
tir. PZR reaksiyonlarında tavuk, keçi, at, kedi DNA’ları pozitif
kontrol, her bir lokus sisteminde DNA negatif PZR reaksiyonları
negatif kontrol olarak kullanılmıştır. Tüm PZR ürünleri
%2 agaroz jel elektroforezinde ayrıştırılmış ve görüntülenmiştir.
İki farklı kanatlı spesifik markör sistemi kullanılarak
yapılan PZR analizleri sonucunda, yem örneğinde
kanatlı DNA’sının varlığı tespit edilmiştir. Ancak, ruminant,
domuz, at ve karnivor DNA varlığı tespit edilememiştir. Bu
çalışma, moleküler biyoloji yöntemlerinin yüksek ısılarda
muamele edilerek hazırlanan rendering ürünlerinin kökeninin
saptanmasında başarıyla kullanılabileceğini göstermektedir.

Anahtar Kelimeler

DNA teknolojisi, rendering, tür tayini

Identification of animal species in rendering products by DNA technology

Öz

After bovine spongiform encephalopathy (BSE) outbreak,
the use of especially ruminant byproducts in animal rations
is under strict control. An animal feedstuff sample was sent
from the Turkish Republic, Mersin Customs Directorate in
order to identify the animal source. The feedstuff was declared
as “hydrolyzed feather meal” in the custom declaration
and health certificates. In microscopic inspections,
pieces of feathers and hollow shafts were observed. As an
alternative method, DNA was extracted by using standard
phenol/chloroform and dodecyltrimethylammonium bromide
(DTAB) methods. The DNA samples were amplified
by Polymerase Chain Reactions (PCR) using poultry, ruminant,
swine, horse and carnivore specific oligos. In PCR
reactions, chicken, goat, horse and cat DNAs were used as
positive controls. DNA free PCR reactions were also amplified
as negative control in each system. The resulting PCR
products were separated and visualized by electrophoresis
on a 2% agarose gel. The findings suggested presence
of poultry DNA in the feedstuff sample since two different
poultry specific PCR primer pairs resulted in positive PCR
products. However, no PCR products were observed in ruminant,
swine, horse and carnivore PCR reactions. The results
of this study thereby indicated that molecular biology
techniques could successfully be used to identify source of
heat proceeded animal byproducts.

Anahtar Kelimeler

DNA technology, rendering, species identification

Kaynakça

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