Subklinik mastitisli ineklerden izole edilen enterokok suşlarının antibiyotik dirençlilikleri

Yıl 2011, Cilt: 27 Sayı: 4, 231 - 234, 01.12.2011

Yahya Kuyucuoğlu

Öz

Amaç: Bu çalışma ile subklinik mastitisli süt ineklerinden izole edilen enterokok suşlarında antibiyotik dirençliliğinin araştırılması amaçlandı. Gereç ve Yöntem: Afyonkarahisar’da bulunan çiftliklerden toplam 392 CMT pozitif süt örneği toplandı. Enterococcus türlerinin tiplendirilmesinde API 20 Strep kiti kullanıldı. Antibiyotik duyarlılıkları National Committee for Clinical Laboratory Standards’ın önerdiği disk difüzyon metoduna göre yapıldı. Bulgular: Sığır mastitislerinden toplam 43 enterococci izole edildi. İzole edilen suşların çoğunluğunu E. faecalis (%53.4) ve E. faecium (%18.6) oluşturdu. Diğer 12 (%27.9) suş sınıflandırılamadı. Bu çalışmada izole edilen E. faecalis suşları, tetrasiklin (%91.3), eritromisin (%82.6) ve penisilin (%78.2)’e dirençli bulundu. Öneri: Bu çalışma ile CMT pozitif süt örneklerinden izole edilen E. faecalis, E. faecium ve diğer Enterococci suşlarında yüksek oranlarda antibiyotik dirençliliği belirlenmiştir. Enterokok suşlarındaki antibiyotik dirençliliği halk sağlığı ve çevre açısından önemsenmelidir.

Anahtar Kelimeler

Enterokok, antibiyotik dirençliliği, mastitis

Antibiotic resistances of enterococci isolated from bovine subclinical mastitis

Öz

Aim: The aim of this study was to investigate the antibiotic resistances of enterococci species isolated from bovine subclinical mastitis in dairy cows. Materials and Methods: A total of 392 CMT positive milk samples were collected from dairy farms in Afyonkarahisar. Typing of enterococcus species were performed using API 20 Strep, subsequently, antibiotic sensitivities were determined by disk diffusion method according to the National Committee for Clinical Laboratory Standards. Results: A total of 43 isolated enterococci, involved in bovine mastitis. The majority of isolates were E. faecalis (53.4%), followed by E. faecium (18.6%). The remaining 12 (27.9%) strains could not be classified. This study observed that the highest percentage of resistance by E. faecalis were to tetracycline (91.3%), erythromycin (82.6%) and penicillin (78.2%). Conclusion: This study showed a high prevalence of antimicrobial resistant E. faecalis, E. faecium and other Enterococci species isolated from CMT positive milk samples. Resistance to antibiotics in enterococci is becoming a concern for human and environmental health.

Anahtar Kelimeler

Enterococci, antimicrobial susceptibility, bovine mastitis

Kaynakça

• Aydın N, Paracıkoğlu J, 2006. Veteriner Mikrobiyoloji (Bak- teriyel Hastalıklar), in; Enterococcus infeksiyonları, Ed; Akan M, İlke Emek Press, Ankara, Turkey, s: 27-29.
• Aarestrup FM, Wegener HC, Rosdahl VT, Jensen, NE, 1995. Staphylococcal and other bacterial species associated with intramammary infections in Danish dairy herds. Acta Vet Scand, 36, 475-487.
• Aarestrup FM, Butaye P, Witte W, 2002. Nonhuman reser- voirs of enterococci, in; The Enterococci: Pathogenesis, Molecular Biology, and Antibiotic Resistance, Eds; Gilm- ore MS, Clewell DB, Courvalin P, Dunny GM, Murray BE, Rice LB, ASM Pres, Washington, USA, pp; 55-99.
• Cengiz S, Tekin O, Akan M, 2011. Mastitislerden izole edilen enterokokların moleküler tiplendirilmesi. Ankara Üniv Vet Fak Derg, 58, 17-20.
• Centers for Disease Control and Prevention (CDC), 2002. Staphylococcus aureus resistant to vancomycin-United States. MMWR, 51, 565-567.
• Chingwaru W, Mpuchane SF, Gashe BA, 2003. E. faecalis and E. faecium isolates from milk, beef and chicken and their antibiotic resistance. J Food Prot, 66, 931-936.
• Courvalin P, 2006. Vancomycin resistance in gram-positive cocci. Clin Infect Dis, 42 (Suppl 1), 25-34.
• Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC, 2002. Veterinary Microbiology and Microbial Disease, Blackwell Publishing Professional, Iowa, USA.
• Daza R, Gutierrez J, Piedrola G, 2001. Antibiotic suscep- tibility of bacterial strains isolated from patients with community-acquired urinary tract infections. Int J Anti- microb Agents, 18, 211-215.
• Devriese LA, Laurier L, De HP, Haesebrouck F, 1992. Entero- coccal and streptococcal species isolated from faeces of calves, young cattle and dairy cows. J Appl Bacteriol 72, 29-31.
• Edrington TS, Carter BH, Friend TH, Hagevoort GR, Poole TL, Callaway TR, Anderson, RC, Nisbet DJ, 2009. Influ- ence of sprinklers, used to alleviate heat stress, on faecal shedding of E. coli O157:H7 and Salmonella and antimi- crobial susceptibility of Salmonella and Enterococcus in lactating dairy cattle. Lett Appl Microbiol, 48, 738-743.
• Hayes JR, English LL, Carr E, Wagner D, S Joseph SS, 2004. Multiple-antibiotic resistance of Enterococcus spp. isolated from commercial poultry production environ- ments. Appl Environ Microbiol, 70, 6005-6011.
• Jayarao BM, Oliver SP, 1992. Aminoglycoside-resistant Streptococcus and enterococcus species isolated from bovine mammary secretions. J Dairy Sci, 75, 991-997.
• Jackson CR, Lombard JE, Dargatz DA, Fedorka-Cray PJ, 2011. Prevalence, species distribution and antimicrobial re- sistance of enterococci isolated from US dairy cattle. Lett Appl Microbiol, 52, 41-48.
• Klare I, Werner G, Witte W, 2001 Enterococci habitats, in- fections, virulence factors, resistances to antibiotics, transfer of resistance determinants. Contrib Microbiol, 8, 108-122.
• Lopes MFS, Ribeiro T, Martins MP, Crespo MT, 2003. Genta- mycin resistance in dairy and clinical enterococcal iso- lates and in reference strains. J Antimicrob Chemother, 53, 214-219.
• Lopes MFS, Ribeiro T, Abrantes M, Figueiredo MJJ, Tenreiro R, Crespo MT, 2005. Antimicrobial resistance profiles of dairy and clinical isolates and type strains of enterococ- ci. Int J Food Microbiol, 103, 191-198.
• Madsen PS, Klastrup O, Olsen J, Pedersen PS, 1974. Herd in- cidence of bovine mastitis in four Danish dairy districts. I. The prevalence and mastitogenic effect of micro-or- ganisms in the mammary glands of cows. Nord Vet Med, 26, 473-482.
• Makovec JA, Ruegg PL, 2003. Antimicrobial resistance of bacteria isolated from dairy cow milk samples submit- ted for bacterial culture: 8905 samples (1994-2001). J Am Vet Med Assoc, 222, 1582-1589.
• Nam, HM, Lim SK, Moon JS, Kang HM, Kim JM, Jang KC, Kim JM, Kang MI, Joo JS, Jung SC, 2010. Antimicrobial resis- tance of enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses and Public Health, 57, 59- 64.
• National Committee for Clinical Laboratory Standards, 2002. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, Second edition, Approved Standard. Document M31-A2, NCCLS, Wayne, PA.
• Pitkala A, Haveri M, Pyorala S, Myllys V, Honkanen-Buzalski T, 2004. Bovine mastitis in Finland 2001 prevalence, distribution of bacteria, and antimicrobial resistance. J Dairy Sci, 87, 2433-2441.
• Quinones D, Goni P, Rubio MC, Duran E, Gomez-Lus R, 2005. Enterococcus spp. isolated from Cuba; species frequen- cy of occurrence and antimicrobial susceptibility pro- file. Diagn Microbiol Infect Dis, 51, 63-67.
• Rogers DG, Zeman DH, Erickson ED, 1992. Diarrhea asso- ciated with Enterococcus durans in calves. J Vet Diagn Invest, 4, 471-472.
• Rossitto PV, Ruiz L, Kikuchi Y, Glenn K, Luiz K, Watts JL Cul- lor JS, 2002. Antibiotic susceptibility patterns for envi- ronmental streptococci isolated from bovine mastitis in central California dairies. J Dairy Sci, 85, 132-138.
• Tenhagen B, Koster G, Wallmann J, Heuwieser W, 2006. Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Branden- burg, Germany. J Dairy Sci, 89, 2542-2551.
• Witte W, Wirth R, Klare I, 1999. Enterococci. Chemotherapy, 45, 135-145.