Akut Tolüen Maruziyetinin Tavşan Beyin Korteksinde Yol Açtığı Toksik Etkiler

Yıl 2017, Cilt: 28 Sayı: 1, 33 - 38, 15.06.2017

Mustafa Çiçek Turgay Şişman

https://doi.org/10.35864/evmd.530094

Cited By: 2

Öz

 Bu çalışmada yüksek dozda tolüenin tavşan beyin korteksi üzerine akut dönemdeki histopatolojik ve biyokimyasal etkilerinin araştırılması amaçlandı. On yedi adet Yeni Zelanda cinsi tavşan (4-6 kg) iki guruba ayrıldı. Grup I kontrol grubu (n=7) olarak kullanılırken Grup II’ye (n=10) yüksek doz Tolüen verildi (876 mg/kg/ip). Üç saatlik deney periyodunun sonunda öldürülen hayvanlardan biyokimyasal analizler için kan serumu ve beyin doku örnekleri % 10’luk nötral formaline alınıp parafine gömülerek kesildi (5 μm). H&E (hematoksilen-eozin), Crystal violet boyamaları, Na, K, GSH, MDA ve NO kan serum seviyeleri analiz edildi. Bu çalışma sonucunda Tolüen verilen tavşanlarda önemli ölçüde histopatolojik bozukluklar ve ciddi amiloit plak oluşumu gözlemlenmiştir. Tolüen grubunda Na, K, MDA, kan serumu seviyelerinde önemli ölçüde artış görülmüşken, GSH ve NO seviyelerinde anlamlı derecede azalma izlenmiştir. Bu çalışma sonucunda yüksek doz tolüenin beyin korteksinde akut dönemde ciddi hasarlar oluşturabileceği gösterilmiştir. 

Anahtar Kelimeler

Tolüen, oksidatif hasar, beyin korteksi, tavşan, amyloid plak

Kaynakça

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