Preparation of antigen and antisera to use for epizootiologic searching investigation in some poultry viral diseases

Yıl 1989, Cilt: 6 Sayı: 4, 35 - 54, 01.12.1989

Aysel Ergün

Öz

Against the some viral disease of ·the chickens (Inf. Bronchitis,
Inf. Laryngothacheitis, I. Bursal Disease, CELO and Avian Encephalomyelitis)
We prepared an antigen and an antiserum touse with
A.G.P. tests. We used C.A.M.of the 9 - 10 days old infected from
SPF flock in ordır to prepare I.B., I.L.T. and CELO antigen. For
the antigen. We used brain and gastro-intestinal tracts· of the infected
embryos. Prepared antigen from the· gastro-intestinal tracts,
gave a positive reaction at A.G.P. But we could· not use the brain
suspention in which prepared with the same method for this test
So we decided that the suspention (in the ultracenrifuge) has to
be purified and concentrated. We prepared I.B.D. antigen from the
B. Fabricius of the infected chicks and have vety good results.
The preparation of the immun serum for the other infections
except I.B.D.; no matter which way we use (I. Venonsly ar I. fracheally)
if the antigen is inoculated more than once We observed
that the number of positive animals increased.

Tavukların Bazı Viral Hastalıklarının Epizootiyolojik Taramasında Kullanılmak Üzere Antigen ve Antiserum Hazırlanması

Öz

Tavukların bazı viral hastalıklarına (I. Bronşit, I. Laringotraheit, I. Bursal Hastalığı ( CELO ve Avian Ansafalomiyelit)karşı A.G.P. testinde kullanılmak üzere antigen ve antiserum hazırlandı. I.B., I.L.T., I.B.D, ve CELO anigenlerinin hazırlanmasında SPF nitelikli 9-10 günlük enfekte embriyolarını korio-allentoik membranlarından yararlanıldı. A. Ensafalomiyelit antigeninde ise enfekte embriyonların beyin ve gastro intestinal sismetinden yararlanıldı. Gastro-interstinal sistemden hazırlanan antigen çok iyi sonuç verdi. Fakat aynı yöntemle hazırlanan beyin süspansiyonu bu test için kullanılamadı ve süspansiyonun ultra santrifüjde purifiye ve konsantre edilmesi gerektiği sonucuna varıldı. Gumboro antigeni ise enfekte piliçlerin B. Fabricius'dan hazırlandı ve çok iyi sonuç alındı.
Immun serum hazırlanmasında ise I.B.D. hariç, diğer enfeksiyonlarda hangi yolla olursa olsun (I. Venoz ve I. Tracheal) antigen , birden fazla inokule edildiğinde presipitin antikor taşıyan pozitif hayvan sayısının arttığı gözlendi.

Anahtar Kelimeler

Tavuk, Tavukların Viral Hastalıkları, Viral Hastalıklar, Epizootiyolojik Tarama, Epizootiyolojik Taramada Kullanılmak Üzere Antigen ve Antiserum Hazırlanması, Aysel ERGÜN, 1989, Etlik Veteriner Mikrobiyoloji Dergisi, A.G.P. testi, I. Bronşit, I. Laringotraheit, I. Bursal, CELO, Avian Ansafalomiyelit

Kaynakça

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