Konjuget Üretiminde Kullanılmak Üzere Hamster - Tavşan ve Merkeplerden Kuduz İmmun Serumun Elde Edilmesi

Yıl 1989, Cilt: 6 Sayı: 5, 49 - 58, 01.12.1989

Orhan Aylan

Öz

Bu çalışmada farklı üç hayvan türünden kuduz hyperimmun serumunun elde edilmesi ve bu hyperimmun serumlardan konjugat hazırlanması amaç edinilmiştir.
Buna göre merkeplerden MICLD 50, 10 3,855 hamsterlerden MICLD 50, 10 3,548 ve tavşanlardan ise MICLD 50, 10 3,756 titrede hyperimmun serumlar elde edilmiştir. Buna bağlı olarak bu üç ayrı serumdan hazırlanan konjugatların tireleri ise Merkep 1/10 , Tavşan 1/14, Hamster 1/12 olarak saptanmıştır.

Anahtar Kelimeler

Kuduz, Kuduz İmmun Serumu, Konjuget Üretimi, Hamster, Tavşan, Merkep, Orhan AYLAN, 1989, Etlik Veteriner Mikrobiyoloji Dergisi

To obtain rabies hyperimmune sera by using hamster; rabbit and donkey to produce conjugate

Öz

To obtain rabies hyperimmune sera by using hamster, rabbit and donkey to produce conjugate.
In this study our aim was to obtain rabies hyperimmune sera by using three different animal species including donkey, rabbit and hamster and to prepare conjugate from them.
As a result of this study we obtained hyperimmune sera in MICLD 50, 10 3,855 for donkeys, MICLD 50, 10 3,548 for hamsters and MICLD 50, 10 3,756 for rabbits.
The titers of conjugates prepared from these three hyperimmune sera were follows.Donkey 1/10, Rabbit 1/14, Hamster 1/12.

Kaynakça

• ATANASiU. P. (1973) : Laboratory Techniques in Rarbies (third editlon) Ouantative Assay . CJnd Potaney Test of Antirabies Serum cınd. lmmunoglobulin. s. 314-318.
• ATANASIU, P., TSIAN.G, H. et VIRAT, J. (1974) Obtention d~lg G anti-nucleocapsides ralbiques. 8urification et conjugaison o la peroxydase ou o l'isothiocyanate de tiuoresc~irie. Ann. Micrdbi.ol. lnst. Pasteur 1e5 8, 85-98.
• ATANASIU, P., PcRıR IN, P., FAVR·E, S., CHEVALLIER, G., TSIANG, H. (1983) : lmmunufluorescence and im:munoperoxidase in the diÖgnoiis ·of rabis. Syniposium international sur l'immunodiagnostic compare des infections virales, Montreal (Canada) Academic ·Press, Ne·w York, s. 141-155.
• BURGU. i. (1988) : Doktora Program ı Ders Notları.
• DEAN; D.J ., ABELSETH, M.C. (1973) : The . Fluorescent Antibody Test. Laborator{ Techniques in Rabies (-rhird Edition) s. 73-84.
• GARDNEıR, P.S., MC OUILUN, J. (1980) : Application of immunofluorescence. Rapid Virus Diagnosis, Second Edition. s. 40-85.
• GÜLEY, M., TUNUS, M., TOKER, M.; ŞENTÜRK, M . (1968) : Floresan Antikor Tekniği. ile Kuduz Teşhisi. Etlik Vet. Bakt. Enst. Dergisi. Sayı 5-6 s. 104-118.
• HIJMAN·s . W. (Ü:I7·5) : Proceedings of Uhe fiHh In ternational Centerence on immunofluorescence and ıRelated Techniques. Annals New York Academy Science 254.
• KUHLMANN, D. (1S84) : Preparation of Immuna Sera and Antibodies. Immuno ·Enzyme Techniques in Oytochemistry Verlag Chemie s. 31-41.
• LEPINE, P .. ATANASIU, P. (1966) : Productiön of Therapeutic Antirabies Serum Laboratory Techniques in Rarb ies WHO Monograph Series No 23, Ge:ıeva.
• NAIRN. R.C. (1976) : Fluorescent Protein Tracing, 4 tn Ed. Churchill Livingstone, Edinburgh, London and New York.
• TRIMARCHI, C.V. and .088-BIE, J. (1974) : Production of rabies fluorescent conjugate' by immunizatian of rabbits with purified rabies antlgen. Bull. World Health Organ. 51, s. 447-449.