Sığırlarda Paratüberkülozun Serolojik, Allerjik ve Bakteriyolojik Muayene Yöntemleri ile Mukayeseli Olarak Araştırılması

Yıl 1995, Cilt: 8 Sayı: 1, 19 - 44, 01.12.1995

Bülent Vural Nuray Atala Mustafa Çelebi

Öz

Paratüberküloz son zamanlarda önem kazanan bir yetiştirme hastalığı olarak ruminantlarda görülmektedir. Bu hastalıktan ileri gelen ekonomik kayıplar önemlidir. Dünyada yaygın olan bu hastalığa ülkemizde de rastlanmaktadır. Çalışmamızda paratüberkülozun çeşitli teşhis yöntemleri ile araştırılması beş ayrı işletmede serolojik, allerjik ve gaita örneklerinin bakteriyolojik yoklaması ile yapıldı ve aşağıdaki sonuçlar alındı.
İşletmelerden temin edilen 5118 kan serumu komplement fikzasyon testi ile işlenip 85 pozitif 30 şüpheli reaksiyon alındı. Allerjik teşhisde PPD avian ve PPD johnin kullanılarak 4923 sığırda yapılan tüberkülin testinde 73 pozitif 19 şüpheli reaksiyon tesbit edildi. Serolojik ve allerjik testlerde reaksiyon alınan 145 sığıra PPD johnin uygulandı ve 52 pozitif , 6 şüpheli reaksiyon alındı. Bu sığılardan temin edilen gaita kültürlerinden 15 adedinde M.paratuberculosis izole edilmiştir; ayrıca rektal mukoza kazıntı örneklerinin mikroskopik yoklamalarında 4 pozitif olgu değerlendirilmişitir.

Anahtar Kelimeler

Sığır, Paratüberküloz, Bülent VURAL, Nuray ATALA, Mustafa ÇELEBİ, 1995, Etlik Veteriner Mikrobiyoloji Dergisi, Yetiştirme Hastalıkları, Paratüberkülozun Araştırılması

A comparative study on serological, allergic and bacteriological examination of paratuberculosıs in cattle.

Öz

Paratüberculosis has recently become an important breed ing disease in
ruminants in many countries since it has caused great economic losses. Being
worldwide, paratuberculosis alsa occurs in our country .
in this study, the use of different diagnostic methods in paratuberculosis
was investigated in 5 different farms by serological and allergical tests as well
as bacteriological examination of feces samples. The results obtained are as
follows :
5118 blood sera from 5 herds were tested with the antigen prepared with
M.paratuberculosis 3 and 5 path strains by complement fixation test and 85
positive and 30 suspected sera were found .
The sera that responded to paratuberculosis were tested with avian antigen
by the complement fixation test and 76 positive and 18 suspected sera were
found .Tuberculin (PPD bovine, PPD avian) administration in 4923 catle resulted
in 73 positives and 19 suspected to PPD avian and no posit ives to PPD bovine.
According to serological and suspected cattle were kept separately. Two
months afterwards, PPD Johnin was administered on them . PPD John in tuberculin
administered on 145 cattle resulted in 52 pozitives and 6 suspected .
15 M. paratuberculosis isolations were made from rectal mucosa and feces
samples from 145 cattle that responded to allerg ical and serological test in
bacteriological examinations (Herold egg . yolk medum with and without
mycobactin) Furthermore, 9 M. pheli isolations were made, causing transient
sensibilisation .
In one management, 21 cattle that reacted to PPD avian were negative for
PPD Johnin and serological tests . Bacteriological examinations offeces samples
from these animals gave negative results. These examinations were carried
out three times with 6 month intervals.
In bacteriological carried out on starlings that are widely present in winter.
Runyon group AAR mycobacteria were isolated. This mycobacterium induce
no lesions in laboratory animals. Tuberculin (PPD bovine. PPD avian and PPD
Johnin were inoculated into guinea pigs in equal doses and positive reaction
occured to PPD avian only.
After tuberculin administration two months later on cattle, positive reaction to PPD avian was observed to have disappeared.
Ina herd , cattle vaccinated against paratuberculosis responded to PPD avian
and Johnin , however, serological tests and PPD bovine results were negative.
Ina herd of 22b0 cattle that hadproblemsin the past with paratuberculosis
allergical tests were carried out (PPD avian and PPD John in were adm inistered
with 6 month interval). In 1990, periodical hygienic applications were made in
this management and 34 cattle positive for paratuberculosis in 1990 and 16
positive cattle in the first s ix months of 1991 and 11 positive cattle in the second
six months of 1991 were slaughtered . Allergical and serological tests carried
out in 1992 and 1993 in complete herd resulted in negative reactions . lt was
concluded that this result was a consequent of the change from range system
to feedlot system .
In the fa rm s on which our'study was carried out no animals with prominent
symptoms of paratuberculosis were observed with an exception that the animals
positive after feces cultures exhibited repeated diarrhea and there was
cachexia in some animals .
In the positive animals, infection could be defined as subclinical or latent
infection of 15 cattle whose feces cultures were positive , 11 were positive , that
is diagnostic efficiency of cultures was 73,2 % i however this was 92,3 % in
PPD Johnin .

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