Civciv Tracheal Organ Kültürlerinde Mycoplasma Gallisepticum' un Etkisi Üzerine Histopatolojik İncelemeler
Yıl 1996,
Cilt: 8 Sayı: 4, 29 - 36, 01.12.1996
Mihriban Ülgen
Ayşin Şen
Deniz Mısırlıoğlu
Öz
Civciv tracheal organ kültürlerinde iki mycoplasma gallisepticum saha
suşumın sitopatojeniteleri incelendi . Tracheal organ kültürleri bir günlük SPF
civcivlerden hazırlandı. Saha suşlarının siliar aktiviteyi durdurma zamanları
referens suş ile paralel bulundu. Histopatolojik incelemede silyalann büyük oranda.
döküldüğü , lamina epitelyalisin yassılaştığı , epitel hücrelerin sitoplazmalarının
vakuolleştiği , çekirdeklerinde piknoz oluştuğu ve bu hücrelerin yerlerinden
döküldüğü gözlendi.
Kaynakça
- ABU-ZAHR, M.N., BUTLER, M., Growth, cytopathogenicity and
morfology ofMycoplasma gallisepticum and M.gallinarum in tracheal explants,
J. Comp. Path., 86, 455-463,1976.
- ARDA, M., İZGÜR, M., Kanatlılarda Mikapiasma İnfeksiynoları , Etlik
Vet. Mikrobiol. Ens. Derg., 6,124-137, 1984.
- AVAKIAN, A.P., LEY, D.H., Protective inunun response to Mycoplasma
gallisepticum deınonstrated in respiratory-tract washing from
M.gallisepticum-infected chickens 37,3,697-705, 1993 .
- CHERRY, J.D., TAYLOR-ROBINSON, D., Large-Quantity Production
Organ Cultures and Use in Virus and Mycoplasma Studies, Applied
Microbiology, 19,658,662, 1970.
- CHERRY, J.D., TAYLOR ROBINSON, D., Mycoplasma Pathogenicity
Studies in Chicken Tracheal Organ Cultures, The Joumal ofPediatrics,
78,6, 1971.
- CERRY, J.D., TAYLOR ROBINSON, D., Groqth and Pathogenicity
Studies of mycoplasına gallisepticum in Chicken Tracheal Organ Cultures,
J.Med.Microbiol., 4,441-449,1971.
- CHERRY, J.D., TAYLOR-ROBINSON, D., Mycoplasına Pathogenicity
Studies in Organ Cultures, Aımals New York Academy of sciences,
225, 290-303, 1973.
- CILLIER, A.M., CARSON, J.L., Tracheal Organ Cultures as Modelsin
Pathogenicity Studies, Methods in Mycoplasınology, editör Vol.ll , Acadeınic
Press, U.S.A., 1983, p: 331-335.
- DYKSTRA, M.J., LEVISOHN, S.FLETCHER, O.J., Evaluation
of cytopathologic changes induced in chicken tracheal epithelium by Mycoplasmagallisepticum
in vivo and in vitro, Am. J. Res., 46, 1, 116, 122, 1985.
- FREY, H.L., HANSON, R.P., ANDERSON, D.P., A Mediuın for
the Isolation of Avian Mycoplasmas, Am.J.Vet.Res., 29, 11 ,2163-2169, 1968.
- HİRANO, H., HAYATSU, E., KUME, K., KAWAKUBO, Y.,
NAKAGAWA, S., NISHIYAMA, Y., YOSHIOKA, M., Ptahogenicity of
Mycoplasına synoviae in Chicken Tracheal Organ Cultures, The Japanese Journal
ofVeterinary Science, 40,2,147-156, 1978.
Histopathological Investigations On Effect Of Mycoplasma Gallisepticum In Chcken Tracheal Organ Cultures
Yıl 1996,
Cilt: 8 Sayı: 4, 29 - 36, 01.12.1996
Mihriban Ülgen
Ayşin Şen
Deniz Mısırlıoğlu
Öz
The cytopathogenicity oftwo Mycoplasma gallisepticum field strains were
investigated in chicken tracheal organ cultures. Tracheal organ cultures were
prepared from one-day old SPF chick. Time of cilia-stopping effect of the strains
were paraUel to that of reference strain. In histopatohological investigation,
marked los s of cilia, fiattening of lanıina epithelialis, vacuolisation, pyknosis and
desquamation of efıthelial cells were observed.
Kaynakça
- ABU-ZAHR, M.N., BUTLER, M., Growth, cytopathogenicity and
morfology ofMycoplasma gallisepticum and M.gallinarum in tracheal explants,
J. Comp. Path., 86, 455-463,1976.
- ARDA, M., İZGÜR, M., Kanatlılarda Mikapiasma İnfeksiynoları , Etlik
Vet. Mikrobiol. Ens. Derg., 6,124-137, 1984.
- AVAKIAN, A.P., LEY, D.H., Protective inunun response to Mycoplasma
gallisepticum deınonstrated in respiratory-tract washing from
M.gallisepticum-infected chickens 37,3,697-705, 1993 .
- CHERRY, J.D., TAYLOR-ROBINSON, D., Large-Quantity Production
Organ Cultures and Use in Virus and Mycoplasma Studies, Applied
Microbiology, 19,658,662, 1970.
- CHERRY, J.D., TAYLOR ROBINSON, D., Mycoplasma Pathogenicity
Studies in Chicken Tracheal Organ Cultures, The Joumal ofPediatrics,
78,6, 1971.
- CERRY, J.D., TAYLOR ROBINSON, D., Groqth and Pathogenicity
Studies of mycoplasına gallisepticum in Chicken Tracheal Organ Cultures,
J.Med.Microbiol., 4,441-449,1971.
- CHERRY, J.D., TAYLOR-ROBINSON, D., Mycoplasına Pathogenicity
Studies in Organ Cultures, Aımals New York Academy of sciences,
225, 290-303, 1973.
- CILLIER, A.M., CARSON, J.L., Tracheal Organ Cultures as Modelsin
Pathogenicity Studies, Methods in Mycoplasınology, editör Vol.ll , Acadeınic
Press, U.S.A., 1983, p: 331-335.
- DYKSTRA, M.J., LEVISOHN, S.FLETCHER, O.J., Evaluation
of cytopathologic changes induced in chicken tracheal epithelium by Mycoplasmagallisepticum
in vivo and in vitro, Am. J. Res., 46, 1, 116, 122, 1985.
- FREY, H.L., HANSON, R.P., ANDERSON, D.P., A Mediuın for
the Isolation of Avian Mycoplasmas, Am.J.Vet.Res., 29, 11 ,2163-2169, 1968.
- HİRANO, H., HAYATSU, E., KUME, K., KAWAKUBO, Y.,
NAKAGAWA, S., NISHIYAMA, Y., YOSHIOKA, M., Ptahogenicity of
Mycoplasına synoviae in Chicken Tracheal Organ Cultures, The Japanese Journal
ofVeterinary Science, 40,2,147-156, 1978.