First molecular detection of Canine Hemoplasmas in Sivas province in central part of Turkey

Yıl 2021, Cilt: 32 Sayı: 1, 27 - 32, 30.06.2021

Ufuk Erol Kürşat Altay Ahmet Duran Ataş Ömer Faruk Şahin Osman Furkan Urhan

https://doi.org/10.35864/evmd.903166

Öz

Canine hemoplasmas are tick-borne bacterial pathogens having worldwide distribution. There are two hemoplasmas species that cause disease in dogs. These are Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum. The aim of this study was to evaluate the prevalence of canine hemoplasmas among apparently healthy 194 owned-dogs in Sivas Province using species-specific polymerase chain reaction (PCR). According to our results, the overall prevalence of canine hemoplasmas was 14.94% (29/194). The molecular prevalence of M. haemocanis and Ca. M. haematoparvum was detected as 8.24% (16/194) and 10.50% (21/194) respectively while the prevalence of co-infections was 4.12% (8/194). In this study, Ca. M. haematoparvum which can infect humans was found more prevalent than M. haemocanis. To the best of our knowledge, it is the first molecular study on the determination of canine hemoplasmas in Sivas province in Turkey.

Anahtar Kelimeler

Canine hemoplasmas, Dog, PCR, Sivas, Turkey

Teşekkür

The authors thank Dr. Mehmet Fatih AYDIN (Department of Public Health, Faculty of Health Sciences, University of Karamanoglu Mehmetbey, Turkey) for providing positive control DNA’s of Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum and also, we are grateful to Veterinarians Anıl Berat Mutlu, Enes KARAKAYA, and Eren ÇAMDALI for their kind help during sample collection.

Sivas ilinde Canine Hemoplasma’nin ilk moleküler tespiti

Öz

Canine hemoplasmas türleri vektör kaynaklı önemli bakteriyel patojenlerdendir. Etkenler dünyanın farklı bölgelerinde evcil köpeklerde tespit edilmiştir. Köpeklerde hastalığa neden olan iki önemli hemoplasma türü vardır bunlar sırasıyla Mycoplasma haemocanis ve Candidatus Mycoplasma haematoparvum’dur. Bu çalışmanın amacı Sivas ilinde 194 evcil köpekte canine hemoplasmas etkenlerinin yaygınlığının Polimeraz Zincir Reaksiyonu ile araştırılmasıdır. Bu çalışmada köpeklerin %14,94 (29/194)’ünde en az bir canine hemoplasmas türü ile enfekte olduğu tespit edilmiştir. Köpeklerin %8,24 (16/194)’ünde M. haemocanis, %10,50 (21/194)’ünde ise Ca. M. haematoparvum tespit edilmiştir. Köpeklerin %4,12 (8/194)’ünde ise miks enfeksiyon görülmüştür. Çalışmada köpeklerde Ca. M. haematoparvum’un M. haemocanis’e göre daha yaygın olduğu tespit edilmiştir. Bu çalışma ile bildiğimiz kadarıyla Sivas ilinde ilk kez köpeklerde moleküler yöntemlerle canine hemoplasmas türlerinin yaygınlığı araştırılmıştır.

Anahtar Kelimeler

Canine hemoplasmas, Köpek, PCR, Sivas, Türkiye

Kaynakça

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