Diazinonun Sıçan Hepatositleri Üzerine Etkisinin Elektron Mikroskobu ile İncelenmesi

Yıl 2004, Cilt: 15 Sayı: 1-2, 29 - 36, 01.12.2004

Yavuz Ulusoy Burhan Toprak , Meltem Uzunhisarcıklı Ayşe Öğütçü

Öz

Diazinon zirai mücadelede yaygın olarak kullanılır ve hem insanlar hem de hayvanlar tarafından sindirim, solunum ve deri yoluyla absorbe olmaktadır. Bu çalışmada 15mg/kg/gün diazinon mısır yağı içinde erkek Wistar ratlara 5 hafta boyunca oral intibasyon yolu ile verilmiştir. Elektron mikroskobuyla yapılan inceleme sonucunda 1. hafta sonunda hepatositlerde patolojik bir durum gözlenmezken, 2. hafta sonunda mitokondrilerde hafif şişme, 3. hafta sonunda mitokondrilerde aşırı şişme, 4.hafta sonunda hepatositlerde şişme, 5. hafta sonunda hepatositlerde piknotik çekirdek, sitoplazmada vakuolizasyon ve mitokondrilerde şişme ve vakuolizasyon meydana gelmiştir.

Anahtar Kelimeler

Diazinon, Pestisit, Karaciğer, Elektron Mikroskobu, Etlik Veteriner Mikrobiyoloji Dergisi, 2004, Yavuz ULUSOY, Burhan TOPRAK, Meltem UZUNHİSARCIKLI, Ayşe ÖĞÜTÇÜ

Electron Microscopic İnvestigation of Effects of Diazinon on Rat's Hepatacytes

Öz

Diazinon is widely used in agricultural control and it is absorbed by both human and animals
through ingestion, inhalation and percutaneously. In this study diazinon was given to male Wistar
rats by oral intubatian 15mg/kg/per day in corn oil for 5 weeks. The result of investigations with electron microscopy: at the end of l week no pathofogical findings was observed, at the end of 2
week weak swelling of mitochondria, at the end of 3rd week extreme swelling ofmitochondria, at the end of 4th week swelling of hepatocyle, at the end of 5'th week picnotic nucfeus in hepatocytes. vacuolization of cytoplasm and swelling and vacuolization of mitochondria were occurred.

Anahtar Kelimeler

Diazinon, pestisicides, liver, electron microscopy

Kaynakça

• ALDANA L, TSUTSUMİ V, CRAİGMİLL A , SİLVEİRA MI, MEJİA EC (2001). t:t Tocopherol modulates liver toxicity of the pyrethroid cypermethrin . Toxicol Lett. 125 ( 1-3 ). 107-116.
• BAILY HC, DEANOVIC L, REYES E, KIMBALL T, LARSON K, CONNER V, HINTON DE (2000). Diazinon and Chlorpyrifos in urban waterways in northern Californi a. USA. Emiran Toxicol C hem, 19. 82-87.
• GALLO M, LAWRYK NJ (1 991 ). Organic phosplıorus pesticides, In: Hayes HI.!, Law (Edv). 1-fandbook of Pesticide Toxicology. p.l 050-1053.
• GARFITT SJ, JONES K, lVIASON HJ, COCKER J (2002). Exposure to the organoplıosphate di azinon : data from a human volunteer study witlı oral and d e rınal doses. Toxicul Lett, 134 (1-3), 105-113.
• HAGAR HH, FAHMY AH (2002). A biochemical , histochamical , and ultrastnıctural evaluation of the effect of dimethoate intoxication onratpancreas. Toxico/Lell. 133 , 161-1 70.
• HANDY RD, ABD-EL SAM EI HA, BA YOMY MFF, MAHRAN AM, ABDEEN AM, ELELAIMY EA (2002). Chronic diazinon exposure: pathologies of spleen, thymus, blood cells. and lymph nodes are modulated by dierary protein or lipid in the mouse. Toxicology , 172 ( 1 ). 13-34.
• IARC (1983). Monograph on the evaluarion of carcinogenic risk of chemicals to man. M iscellaneous Pesticides. vo l. 30. International Agency for Research on Cancer. Lyon. France.
• JOHN S, KALE M, RATHORE N, BHA TN ACAR D (200 1 ). Prorective effect of vitamin E in dimethoate and malathian induced oxidative stress in rat erythrocytes .J Nutr Biochem. 12, 500-504.
• JOSHI SC, MATHUR R, GAJRAJ A, SHARMA T (2003 ). lntluence of metiyl parathion on reproductive parameters in male rars. Environ Toxicol Pharm, 14, 91-98.
• KALENDER S, KALENDER Y, ÖGÜTCÜ A, UZUNHISARCIKLI M, DURAK D, AÇIKGÖZ F (2004). Endosulfan-induced cardiotoxicity and free radical metabolism in rats: The protective effect of vitamin E. Toxicologv 202 (3). 227-235 .
• KALENDER Y, KALENDER S. UZUNHISARCIKLI M, OGUTCÜ A, AÇIKGÖZ F, DURAK D (2004) Effects of endosulfan on B cells of Langerhans islets in rat pancreas. Toxicology. 200 (2-3 ). 205-21 1.
• KAPPERS WA, EDWARDS RJ, MURRA Y S, BOOBIS AR (200 1 ). Diazinon is activated by CYP2C 19 in Human Li ver. Toxicol Appl Pharm, 177, 68-76.
• KEIZER J, NAGEL GDR, TERESA VOLPE T, PAOLA GNEMI P, LUCIANO VITTOZZI L ( 1995). Enzyınological differeııces of AChE and diazinon hepatic ınetabolism : correlation of in vitro data with the selective toxicity of diazinon to fish species. Sc i Total Environ. 171 ( 1 -3), 213-220.
• MOUSSA TA, HAFEZ MM (1995). The effect of dimethoate on the mitochondria of the guinea pig. Egypt J Histol. 6, 1 O 1-106.
• NAKAGAWA Y, MOORE G (1999). Role of ınitochondrial ıneınbrane permeability transition in p-hydroxybenzoate ester-induced cytotoxicity in rat hepatocytes. Biochem Pharmacol, 58 (5), 81 1-816.
• NEISHABOURI EZ, HASSAN ZM, AZIZI E, OST AD SN (2004). Evaluation of iınınunotoxicity induced by diazinon in C57b1/6 mice. Toxicology, 196(3). 173-179.
• RODRIGON L, HERNANDEZ AF, LÖPEZCABALLERO JJ, GIL F, PLA A (200 1 ).lmınunohistocheınical ev idence for the expressian and induction of paraoxonase in rat liver, kidney, lung and brain tissue. implications for its physiological role. Chem-Biol lnteract, 1 3 7 (2 ). 1 23- 1 3 7.
• SAMS C, COCKER J, LENNARD MS (2003). Metabolisın of chlorpyrifos and diazinon by human liver microsomes. Toxicol Left, 144, 146.
• SELMANOGLU G, BARLAS N, SONGUR S. KOCKAYA EA (200 1 ). Carbendaziın-inclucecl haeınatological , biochemical and histopathological changes to the li ver and kidney of ınale rats. Hum Exp Toxicol, 20 (12). 625-630.
• SULTATOS LG (1994). Mamınalian toxicologv or organophosphorus pesticides. J Toxicol Env Health, 43 (3), 271-289.
• WHO 1998. Diazinon. Environmental Health Criteria 198. International Program on Chemical Safety. United Nations Environınental Program. World Health Organization, Geneva. Switzerland.