Araştırma Makalesi
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Atık fetus mide içeriklerinden konvansiyonel kültürel yöntem ve polimeraz zincir reaksiyonu ile Brucella spp.’nin teşhisi

Yıl 2009, Cilt: 20 Sayı: 1-2, 35 - 38, 31.12.2009

Öz

Brusellosis, Brucella türlerinin neden olduğu oldukça bulaşıcı, zoonotik bir enfeksiyondur. Brusellozisin teşhisinde,
serolojik ve konvansiyonel kültürel yöntemlerin yanı sıra farklı polimeraz zincir reaksiyonu (PZR) uygulamaları
da kullanılmaktadır. Bu çalışmada 31 adet sığır, 8 adet koyun ve 1 adet keçi fötal atık mide içeriğinden bakteriyolojik
kültür ve PZR ile Brucella spp.’nin belirlenmesi ve her iki teşhis yönteminin karşılaştırılması amaçlanmıştır. Çalışma
sonunda her iki yöntemle de benzer pozitif ve negatif sonuçlar elde edilmiştir.

Kaynakça

  • Adone R, Ciuchini F, La Rosa G, Marianelli C, Muscillo M, (2001). Use of polymerase chain reaction to identify Brucella abortus strain RB51 among Brucella field isolates from cattle in Italy. J Vet Med B Infect Dis Vet Public Health. 48(2), 107-113.
  • Alton GG, Jones LM, Angus RD, Verger JM, (1988). Techniques for the Brucellosis Laboratory. Institut National de la Recherche Agronomique, Paris, France.
  • Amin AS, Hamdy ME, Ibrahim AK, (2001). Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Vet Microbiol. 83(1), 37-44.
  • Aydın N, Paracıkoğlu J, (2006). Veteriner Mikrobiyoloji (Bakteriyel Hastalıklar). Ankara: İlke-Emek Matbaacılık ve Yayıncılık, p. 145-163.
  • Bricker BJ, Halling SM, (1994). Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol. 32(11), 2660-2666.
  • Çetinkaya B, Öngör H, Muz A, Ertaş HB, Kalender HM, Erdoğan HM, (1990). Detection of Brucella species DNA in the stomach content of aborted sheep fetuses by PCR. Vet Rec. 144, 239-240.
  • Fekete A, Bantle JA, Halling SM, Sanborn MR, (1990). Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J Appl Bacteriol. 69(2), 216-227.
  • Guarino A, Serpe L, Fusco G, Scaramuzzo A, Gallo P, (2000). Detection of Brucella species in buffalo whole blood by gene-specific PCR. Vet Rec. 147, 634–636.
  • Güler L, Gündüz K, Ok Ü, (2003). Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol. 93, 53-61.
  • Gürtürk K, Alan M, Boynukara B, Solmaz H, Aksakal A, (1998). Van ve yöresinde koyunlarda brusellozis üzerine etiyolojik ve serolojik incelemeler. Ulusal Sığır ve Koyun Yavru Atma Sempozyumu, 6-8 Ekim, Pendik.
  • Herman L, De Ridder H, (1992). Identification of Brucella spp. by using the polymerase chain reaction. Appl Environ Microbiol. 58(6), 2099-2101.
  • Hesterberg UW, Bagnall R, Perrett K, Bosch B, Horner R, Gummow B, (2008). A serological prevalence survey of Brucella abortus in cattle of rural communities in the province of KwaZulu-natal, South Africa. J S Afr Vet Assoc. 79(1), 15-18.
  • Hinić V, Brodard I, Thomann A, Holub M, Miserez R, Abril C, (2009). IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology. BMC Vet Res. 14(5), 22.
  • Kazemi B, Yousefi Namin SA, Dowlatshahi M, Bandepour M, Kafilzadeh F, Gachkar L, Mahmoudinejad F, Samarghandi A, Mardani M, (2008). Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases. Iranian J Publ Health. 37(4), 96-102.
  • Leal-Klevezas DSL, Vazques IOM, Merino AL, Soriano JPM, (1995). Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol. 33, 3087-3090.
  • Mayfield JE, Bricker BJ, Godfrey H, Crosby RM, Knight DJ, Halling SM, Balinsky D, Tabatabai LB, (1988). The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein. Gene. 63(1), 1-9.
  • Romero C, Gamazo C, Pardo M, López-Goñi I, (1995). Specific detection of Brucella DNA by PCR. J Clin Microbiol. 33(3), 615-617.
  • Romero C, Lopez-Goni I, (1999). Improved method for purification of bacterial DNA from bovine milk for detection of Brucella sp. by PCR. Applied Environ Microbiol. 3735-3737.
  • Surucuoglu S, El S, Ural S, Gazi H, Kurutepe S, Taskiran P, Yurtsever SG, (2009). Evaluation of realtime PCR method for rapid diagnosis of brucellosis with different clinical manifestations. Pol J Microbiol. 58(1), 15-9.
  • Sreevatsan S, Bookout JB, Ringpis F, Perumaalla VS, Ficht TA, Adams LG, Hagius SD, Elzer PH, Bricker BJ, Kumar GK, Rajasekhar M, Isloor S, Barathur RR, (2000). A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle. J Clin Microbiol. 38(7), 2602-2610. Geliş Tarihi / Received: 15.12.2008 Kabul Tarihi / Accepted: 25.12.2009

Diagnosis of Brucella spp. by conventional cultural method and polymerase chain reaction in stomach contents of aborted fetuses

Yıl 2009, Cilt: 20 Sayı: 1-2, 35 - 38, 31.12.2009

Öz

Brucellosis is a contagious, zoonotic infection caused by Brucella species. Beside the serological and conventional
cultural methods, different polymerase chain reaction (PCR) applications can be used in diagnosis of brucellosis.
The aim of this study was to compare bacteriological culture and PCR methods used for the detection of
Brucella spp. in stomach contents of the 31 bovine, 8 ovine and 1 caprine aborted fetuses. At the end of the study,
same positive and negative results were obtained from both methods.

Kaynakça

  • Adone R, Ciuchini F, La Rosa G, Marianelli C, Muscillo M, (2001). Use of polymerase chain reaction to identify Brucella abortus strain RB51 among Brucella field isolates from cattle in Italy. J Vet Med B Infect Dis Vet Public Health. 48(2), 107-113.
  • Alton GG, Jones LM, Angus RD, Verger JM, (1988). Techniques for the Brucellosis Laboratory. Institut National de la Recherche Agronomique, Paris, France.
  • Amin AS, Hamdy ME, Ibrahim AK, (2001). Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Vet Microbiol. 83(1), 37-44.
  • Aydın N, Paracıkoğlu J, (2006). Veteriner Mikrobiyoloji (Bakteriyel Hastalıklar). Ankara: İlke-Emek Matbaacılık ve Yayıncılık, p. 145-163.
  • Bricker BJ, Halling SM, (1994). Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol. 32(11), 2660-2666.
  • Çetinkaya B, Öngör H, Muz A, Ertaş HB, Kalender HM, Erdoğan HM, (1990). Detection of Brucella species DNA in the stomach content of aborted sheep fetuses by PCR. Vet Rec. 144, 239-240.
  • Fekete A, Bantle JA, Halling SM, Sanborn MR, (1990). Preliminary development of a diagnostic test for Brucella using polymerase chain reaction. J Appl Bacteriol. 69(2), 216-227.
  • Guarino A, Serpe L, Fusco G, Scaramuzzo A, Gallo P, (2000). Detection of Brucella species in buffalo whole blood by gene-specific PCR. Vet Rec. 147, 634–636.
  • Güler L, Gündüz K, Ok Ü, (2003). Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol. 93, 53-61.
  • Gürtürk K, Alan M, Boynukara B, Solmaz H, Aksakal A, (1998). Van ve yöresinde koyunlarda brusellozis üzerine etiyolojik ve serolojik incelemeler. Ulusal Sığır ve Koyun Yavru Atma Sempozyumu, 6-8 Ekim, Pendik.
  • Herman L, De Ridder H, (1992). Identification of Brucella spp. by using the polymerase chain reaction. Appl Environ Microbiol. 58(6), 2099-2101.
  • Hesterberg UW, Bagnall R, Perrett K, Bosch B, Horner R, Gummow B, (2008). A serological prevalence survey of Brucella abortus in cattle of rural communities in the province of KwaZulu-natal, South Africa. J S Afr Vet Assoc. 79(1), 15-18.
  • Hinić V, Brodard I, Thomann A, Holub M, Miserez R, Abril C, (2009). IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology. BMC Vet Res. 14(5), 22.
  • Kazemi B, Yousefi Namin SA, Dowlatshahi M, Bandepour M, Kafilzadeh F, Gachkar L, Mahmoudinejad F, Samarghandi A, Mardani M, (2008). Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases. Iranian J Publ Health. 37(4), 96-102.
  • Leal-Klevezas DSL, Vazques IOM, Merino AL, Soriano JPM, (1995). Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol. 33, 3087-3090.
  • Mayfield JE, Bricker BJ, Godfrey H, Crosby RM, Knight DJ, Halling SM, Balinsky D, Tabatabai LB, (1988). The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein. Gene. 63(1), 1-9.
  • Romero C, Gamazo C, Pardo M, López-Goñi I, (1995). Specific detection of Brucella DNA by PCR. J Clin Microbiol. 33(3), 615-617.
  • Romero C, Lopez-Goni I, (1999). Improved method for purification of bacterial DNA from bovine milk for detection of Brucella sp. by PCR. Applied Environ Microbiol. 3735-3737.
  • Surucuoglu S, El S, Ural S, Gazi H, Kurutepe S, Taskiran P, Yurtsever SG, (2009). Evaluation of realtime PCR method for rapid diagnosis of brucellosis with different clinical manifestations. Pol J Microbiol. 58(1), 15-9.
  • Sreevatsan S, Bookout JB, Ringpis F, Perumaalla VS, Ficht TA, Adams LG, Hagius SD, Elzer PH, Bricker BJ, Kumar GK, Rajasekhar M, Isloor S, Barathur RR, (2000). A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle. J Clin Microbiol. 38(7), 2602-2610. Geliş Tarihi / Received: 15.12.2008 Kabul Tarihi / Accepted: 25.12.2009
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

H. Kaan Müştak Bu kişi benim

Elçin Günaydın Bu kişi benim

Uğur Küçükayan Bu kişi benim

Asiye Dakman Bu kişi benim

Yayımlanma Tarihi 31 Aralık 2009
Gönderilme Tarihi 1 Aralık 2009
Yayımlandığı Sayı Yıl 2009 Cilt: 20 Sayı: 1-2

Kaynak Göster

APA Müştak, H. K., Günaydın, E., Küçükayan, U., Dakman, A. (2009). Atık fetus mide içeriklerinden konvansiyonel kültürel yöntem ve polimeraz zincir reaksiyonu ile Brucella spp.’nin teşhisi. Etlik Veteriner Mikrobiyoloji Dergisi, 20(1-2), 35-38.


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