Total Phenolic and Flavonoid Contents of Ethanol Extract of Manilkara kauki Leaves and Antibiofilm Activity Test Against Biofilms of Pseudomonas aeruginosa
Öz
One of the infectious bacteria that can cause high morbidity and mortality is Pseudomonas aeruginosa. P. aeruginosa is a difficult pathogen to treat and often causes resistance to antibiotics. One of the causes of antibiotic resistance is due to the formation of biofilm. Manilkara kauki is one of the plants that has potential as an antibiofilm. The absence of publications related to information on total phenolic and flavonoid contents and the use of M. kauki leaves as antibiofilm makes this research important and necessary. This study aims to determine the total phenolic and flavonoid contents in the ethanol extract of M. kauki leaves and antibiofilm activity tests which include optimisation of biofilm formation time, inhibition activity test, and biofilm destruction using the Microtiter Plate Assay method with positive control of ciprofloxacin. The results showed that the total phenolic and flavonoid contents in the ethanol extract of M. kauki leaves were 48.9830 mg/g GAE and 21.3270 mg/g QE, respectively. The results of the antibiofilm activity test showed that P. aeruginosa is a strong biofilm forming bacterium with an optimum formation time of 48 hour and an OD value of 0.5298. Meanwhile, the highest percentage of biofilm inhibition and destruction at concentration 1000 ppm with IC50 values of 134 and 108.4 μg/mL.
Anahtar Kelimeler
antibiofilm flavonoid phenolic Pseudomonas aeruginosa Manilkara kauki
Proje Numarası
-
Kaynakça
- Agustin, A., Widayanti, E., Ikayanti, R., & Kesuma, S. (2022). Penetapan kadar fenol total ekstrak etanol berbagai biji buah salak bali (Salacca zalanca var. Ambonensis) menggunakan metode Folin Ciocalteu. Journal of Nutrition, 1(3), 19–25. https://doi.org/10.31290/nj.v1i3.3705
Total Phenolic and Flavonoid Contents of Ethanol Extract of Manilkara kauki Leaves and Antibiofilm Activity Test Against Biofilms of Pseudomonas aeruginosa
Öz
One of the infectious bacteria that can cause high morbidity and mortality is Pseudomonas aeruginosa. P. aeruginosa is a difficult pathogen to treat and often causes resistance to antibiotics. One of the causes of antibiotic resistance is due to the formation of biofilm. Manilkara kauki is one of the plants that has potential as an antibiofilm. The absence of publications related to information on total phenolic and flavonoid contents and the use of M. kauki leaves as antibiofilm makes this research important and necessary. This study aims to determine the total phenolic and flavonoid contents in the ethanol extract of M. kauki leaves and antibiofilm activity tests which include optimisation of biofilm formation time, inhibition activity test, and biofilm destruction using the Microtiter Plate Assay method with positive control of ciprofloxacin. The results showed that the total phenolic and flavonoid contents in the ethanol extract of M. kauki leaves were 48.9830 mg/g GAE and 21.3270 mg/g QE, respectively. The results of the antibiofilm activity test showed that P. aeruginosa is a strong biofilm forming bacterium with an optimum formation time of 48 hour and an OD value of 0.5298. Meanwhile, the highest percentage of biofilm inhibition and destruction at concentration 1000 ppm with IC50 values of 134 and 108.4 μg/mL.
Anahtar Kelimeler
antibiofilm flavonoid phenolic Pseudomonas aeruginosa Manilkara kauki
Etik Beyan
-
Destekleyen Kurum
-
Proje Numarası
-
Teşekkür
-
Kaynakça
- Agustin, A., Widayanti, E., Ikayanti, R., & Kesuma, S. (2022). Penetapan kadar fenol total ekstrak etanol berbagai biji buah salak bali (Salacca zalanca var. Ambonensis) menggunakan metode Folin Ciocalteu. Journal of Nutrition, 1(3), 19–25. https://doi.org/10.31290/nj.v1i3.3705
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Eczacılık Bilimleri |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | - |
| Gönderilme Tarihi | 28 Temmuz 2025 |
| Kabul Tarihi | 27 Şubat 2026 |
| Yayımlanma Tarihi | 31 Mart 2026 |
| DOI | https://doi.org/10.55262/fabadeczacilik.1752400 |
| IZ | https://izlik.org/JA26BC83FL |
| Yayımlandığı Sayı | Yıl 2026 Cilt: 51 Sayı: 1 |