Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro

Yıl 2022, Cilt: 1 Sayı: 47, 13 - 22, 01.03.2022

Seda İpek İrem İyigündoğdu Aylin Üstündağ , Yalçın Duydu

https://doi.org/10.55262/fabadeczacilik.1078759

Cited By: 1

Öz

Endocrine-disrupting chemicals like bisphenol A (BPA) and its analogs have negative effects on human health. This research aims to determine the cytotoxic effects of BPA and its four different analogs bisphenol S (BPS), bisphenol F (BPF), bisphenol Z (BPZ), bisphenol AF (BPAF) on both cancer and healthy cell lines simultaneously by performing an MTT test. In this study, human breast cancer cells (MCF-7) and human Sertoli cells (HSeC) were used for cell culture. MCF-7 and HSeC were exposed to BPA, BPS, BPF, BPZ, BPAF for 24 h. After that, the inhibitory effect of Bisphenols (IC50) was determined by measuring the absorbance. While BPF was the least cytotoxic alternative depending on the highest IC50 values in both cell lines, BPZ was found to be the most cytotoxic alternative in HSeC cell line. In the MCF-7 cell line, BPA and BPZ were found to have equally cytotoxic effects.

Anahtar Kelimeler

Endocrine-disrupting chemicals, bisphenols, cytotoxicity, MTT, MCF-7 cell lines, HSeC cell lines

Bisfenol A ve Analoglarının MCF-7 ve HSeC Hücre Hatlarında in vitro Sitotoksik Etkisinin Değerlendirilmesi

Öz

Bisfenol A (BPA) gibi endokrin bozucu kimyasallar ve benzerleri insan sağlığı üzerinde olumsuz etkilere sahiptir. Bu araştırma, BPA ve dört farklı analogunun bisphenol S (BPS), bisfenol F (BPF), bisfenol Z (BPZ), bisfenol AF (BPAF) hem kanser hem de sağlıklı hücre hatları üzerindeki sitotoksik etkilerini MTT testi yaparak aynı anda belirlemeyi amaçlamaktadır. Bu çalışmada, hücre kültürü için insan meme kanseri hücreleri (MCF-7) ve insan sertoli hücreleri (HSeC) kullanılmıştır. MCF-7 ve HSeC, 24 saat BPA, BPS, BPF, BPZ, BPAF’ye maruz bırakılmıştır. Daha sonra, bisfenollerin (IC50) inhibitör etkisi absorbans ölçümü ile belirlenmiştir. En yüksek IC50 değerleri nedeniyle her iki hücre hattında BPF en az sitotoksik alternatif iken, BPZ’nin HSeC hücre hattında en sitotoksik alternatif olduğu bulunmuştur. MCF-7 hücre hattında, BPA ve BPZ’nin eşit sitotoksik etkilere sahip olduğu bulunmuştur.

Anahtar Kelimeler

Endokrin bozucu kimyasallar, bisfenoller, sitotoksisite, MTT, MCF-7 hücre hatları, HSeC hücre hatları

Kaynakça

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