Quercetin Based Standardization Of Polyherbal Anti-Gout Remedy And İts Molecular Docking Study Against Anti-Gout And Anti-İnflammatory Protein Targets

Yıl 2022, Cilt: 47 Sayı: 3, 317 - 330, 19.10.2022

Ayisha Shaukat , Khalid Hussain

https://doi.org/10.55262/fabadeczacilik.1085825

Cited By: 1

Öz

A five-herb containing traditional homemade medicine is extensively used to treat gout but has not been standardized for quercetin content. Therefore, the current study describes a reversed-phase liquid chromatographic method for quercetin determination in traditional herbal remedy. The elution was carried out using aqueous 2.0% acetic acid, acetonitrile and tetrahydrofuran (55:40:5, V/V/V) as mobile phase at flow rate of 0.8 mL/min and detection was performed using diode array detector operated at 370 nm. The response of the detector was linear in the range investigated (2.5-160.0 µg/mL) with R2 = 0.996. Results of recovery (98.26-103.22%, SD<5%), intraday accuracy and precision (94.68-104.08%, RSD<5%) and interday accuracy and precision (92.31-104.92%, RSD<5%) showed that the method was reliable, repeatable and reproducible, hence may be used for determination of quercetin in herbal remedy. The medicine contained 0.2425 mg/g quercetin. The molecular interactions of this marker compound were also studied against anti-gout and anti-inflammatory protein targets. Hence, the developed RP-HPLC method may be used to produce standardized anti-gout medicine for quercetin content. Moreover, the molecular interactions help in understanding underlying mechanism of action of this marker compound against gout.

Anahtar Kelimeler

RP-HPLC, standardization, polyherbal, quercetin

Kaynakça

• Careri, M., Corradini, C., Elviri, L., Nicoletti, I., & Zagnoni, I. (2003). Direct HPLC analysis of quercetin and trans-resveratrol in red wine, grape, and winemaking byproducts. Journal of Agricultural and Food Chemistry, 51(18), 5226-5231.
• Chen, X.Q., Xiao, J.B. (2010). RP-HPLC-DAD detrmination of flavonoids: separation of quercetin, luteolin and apigenin in Marchantia convoluta. Iranian Journal of Pharmaceutical Research, (3), 175-181.
• Dmitrienko, S. G., Kudrinskaya, V. A., & Apyari, V. V. (2012). Methods of extraction, preconcentration, and determination of quercetin. Journal of Analytical Chemistry, 67(4), 299-311.
• Duan, Y. (2014). Ultraviolet-visible spectrum characterizations of quercetin in aqueous ethanol solution with different pH values. Journal of Chemical and Pharmaceutical Research, 6(9), 236-240.
• Ewais, E. A., Abd El-Maboud, M. M., Elhaw, M. H., & Haggag, M. I. (2016). Phytochemical studies on Lycium schweinfurthii var. schweinfurthii (Solanaceae) and Isolation of five Flavonoids from leaves. Journal of Medicinal Plant Studies, 4, 288-300.
• Fasolo, D., Schwingel, L., Holzschuh, M., Bassani, V., & Teixeira, H. (2007). Validation of an isocratic LC method for determination of quercetin and methylquercetin in topical nanoemulsions. Journal of Pharmaceutical and Biomedical Analysis, 44(5), 1174-1177. Ferrandiz, M. L., & Alcaraz, M. (1991). Anti-inflammatory activity and inhibition of arachidonic acid metabolism by flavonoids. Agents and Actions, 32(3), 283-288.
• Goo, H. R., Choi, J. S., & Na, D. H. (2009). Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversed-phase high-performance liquid chromatography. Archives of Pharmacal Research, 32(2), 201-206.
• Ishii, K., Furuta, T., & Kasuya, Y. (2003). High-performance liquid chromatographic determination of quercetin in human plasma and urine utilizing solid-phase extraction and ultraviolet detection. Journal of Chromatography B, 794(1), 49-56.
• Kalyaanamoorthy, S., Chen, Y.P.P. (2011). Structure-based drug design to augment hit discovery. Drug Discovery Today, 16(17-18), 831-839.
• Kim, H. P., Mani, I., Iversen, L., & Ziboh, V. A. (1998). Effects of naturally-occurring flavonoids and biflavonoids on epidermal cyclooxygenase and lipoxygenase from guinea-pigs. Prostaglandins, Leukotrienes and Essential Fatty acids, 58(1), 17-24.
• Laughton, M. J., Evans, P. J., Moroney, M. A., Hoult, J. R. S., & Halliwell, B. (1991). Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic dietary additives: relationship to antioxidant activity and to iron ion-reducing ability. Biochemical Pharmacology, 42(9), 1673-1681.
• Li, S., Han, Q., Qiao, C., Song, J., Lung Cheng, C., & Xu, H. (2008). Chemical markers for the quality control of herbal medicines: an overview. Chinese medicine, 3(1), 1-16.
• Lin, C. M., Chen, C. S., Chen, C. T., Liang, Y. C., & Lin, J. K. (2002). Molecular modeling of flavonoids that inhibits xanthine oxidase. Biochemical and Biophysical Research Communications, 294(1), 167-172.
• Lin, S., Zhang, G., Liao, Y., Pan, J., Gong, D. (2015). Dietary flavonoids as xanthine oxidase inhibitors: Structure–affinity and structure–activity relationships. Journal of Agricultural and Food Chemistry, 63(35), 7784-7794
• Liu, H. P., Shi, X. F., Zhang, Y. C., Li, Z. X., Zhang, L., & Wang, Z. Y. (2011). Quantitative analysis of quercetin in Euphorbia helioscopia L by RP-HPLC. Cell Biochemistry and Biophysics, 61(1), 59-64.
• Meng, X. Y., Zhang, H. X., Mezei, M., & Cui, M. (2011). Molecular docking: a powerful approach for structure-based drug discovery. Current Computer-aided Drug design, 7(2), 146-157. 12
• Nessa, F., Ismail, Z., & Mohamed, N. (2010). Xanthine oxidase inhibitory activities of extracts and flavonoids of the leaves of Blumea balsamifera. Pharmaceutical Biology, 48(12), 1405-1412.
• Nile, S. H., Nile, A. S., Keum, Y. S., & Sharma, K. (2017). Utilization of quercetin and quercetin glycosides from onion (Allium cepa L.) solid waste as an antioxidant, urease and xanthine oxidase inhibitors. Food Chemistry, 235, 119-126.
• Phani, C. R., Vinaykumar, C., Rao, K. U., & Sindhuja, G. (2010). Quantitative analysis of quercetin in natural sources by RP-HPLC. International Journal of Research in Pharmaceutical and Biomedical Sciences, 1(1), 19-22.
• Rasoulzadeh, F., Jabary, H. N., Naseri, A., & Rashidi, M. R. (2009). Fluorescence quenching study of quercetin interaction with bovine milk xanthine oxidase. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 72(1), 190-193.
• Rathee, P., Chaudhary, H., Rathee, S., Rathee, D., Kumar, V., & Kohli, K. (2009). Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflammation & Allergy-Drug Targets, 8(3), 229-235.
• Savic, I. M., Nikolic, V. D., Savic, I. M., Nikolic, L. B., & Stankovic, M. Z. (2013). Development and validation of a new RP-HPLC method for determination of quercetin in green tea. Journal of Analytical Chemistry, 68(10), 906-911.
• Shaukat, A., Hussain, K., Bukhari, N.I., Shehzadi, N., Naheed, S., Saghir, F., Iftikhar, S, Javed, O. (2020). In vitro anti-gout and anti-inflammatory activity of traditionally used polyherbal anti-gout remedy. International Journal of Biosciences, 16(5), 327-335.
• Shivashankar, S., Murali, A., & Sangeetha, M. K. (2019). Molecular interaction of phytochemicals with snake venom: Phytochemicals of Andrographis paniculata inhibits phospholipase A2 of Russell's viper (Daboia russelli). Biocatalysis and Agricultural Biotechnology, 18, 101058.
• Sladkovsky, R., Solich, P., Opletal, L. (2001). Simultaneous determination of quercetin, kaempferol and (E)-cinnamic acid in vegetative organs of Schisandra chinensis Baill. by HPLC. Journal of Pharmaceutical and Biomedical Analysis, 24(5-6), 1049-1054.
• Yao, L. H., Jiang, Y. M., Shi, J., Tomas-Barberan, F. A., Datta, N., Singanusong, R., & Chen, S. S. (2004). Flavonoids in food and their health benefits. Plant Foods for Human Nutrition, 59(3), 113-122.
• Zhu, J. X., Wang, Y., Kong, L. D., Yang, C., & Zhang, X. (2004). Effects of Biota orientalis extract and its flavonoid constituents, quercetin and rutin on serum uric acid levels in oxonate-induced mice and xanthine dehydrogenase and xanthine oxidase activities in mouse liver. Journal of Ethnopharmacology, 93(1), 133-140.