Glycosides Isolated from Leaf Extract of Phyllanthus muellerianus (Kuntze) Excell (Phyllanthaceae) Upregulated Cell-mediated Innate Immunity

Yıl 2023, Cilt: 48 Sayı: 1, 11 - 24, 01.03.2023

Martha Nneoma Ofokansi Ogechukwu N Isiogugu Ikechukwu E Peter Matthas Agbo , Festus B.c. Okoye Peter A Akah

https://doi.org/10.55262/fabadeczacilik.1083066

Öz

Intracellular pathogens are mainly eliminated by cell-mediated immunity offered by phagocytic cells like neutrophils, macrophages and monocytes. Two tannin glycosides, 1-O-galloyl-6-O-luteoyl-α-D-glucopyranoside (1) and 3-O-methylellagic acid 3'-O-α-rhamnopyranoside (2) were isolated from the ethyl acetate fraction (EF) of the methanol leaf extract of Phyllanthus muellerianus. The structures of the isolated compounds were elucidated by 1H-NMR and by mass spectroscopy. The effects of the isolated compounds on phagocytic competence of macrophages and neutrophils using in vitro models were evaluated. Compounds 1 and 2 significantly (p<0.05) increased both phagocytic ability and intracellular killing capacity of neutrophils. This study established that the tannin glycosides stimulated cell-mediated innate immunity by increasing the phagocytic function of neutrophils and thus may be useful in both the clinical and prophylactic treatment of intracellular microbial infections.

Anahtar Kelimeler

Glycosides, Cell-mediated, Immunity, Phygocytosis, Neutriphills, Macrophages

Destekleyen Kurum

Institute of Organic Chemistry, Heinrich-Heine University Dusseldorf

Proje Numarası

Nil

Teşekkür

Prof John Ihedioha for granting us a bench space in his laboratory. Dr. W. Peters of the Institute of Organic Chemistry, Heinrich-Heine University Dusseldorf, is appreciated for assisting with the NMR measurements.

Kaynakça

• Agbo M.O., Odimegwu D.C., Okoye F.B.C., Osadebe P.O. (2017). Antiviral activity of Salidroside from the leaves of Nigerian Mistletoe (Loranthus micranthus Linn) parasitic on Hevea brasiliensis against respiratory syncytial virus. Pak. J. Pharm. Sci, 30(4):1251-1256.