NLRP3 expression in peripheral blood mononuclear cells of patients with rheumatoid arthritis

Yıl 2023, Cilt: 48 Sayı: 3, 481 - 492, 18.10.2023

Sezen Yılmaz Sarıaltın , Orhan Küçükşahin , Cemil Nural Leyla Didem Kozacı , Pınar Kaygın , Gülçin Güler Şimşek , Serpil Oğuztüzün , Tülay Çoban

https://doi.org/10.55262/fabadeczacilik.1346586

Öz

Rheumatoid arthritis (RA) is a systemic autoimmune disease mainly characterized by painful, swollen, and inflamed joints. Individual, genetic, and environmental factors influence the development of the disease, which causes involvement not only in the joints but also in other extra-articular tissues. However, its etiopathogenesis has not been fully elucidated yet. NLRP3, which is a key component of innate immune system, may contribute to recurrent and chronic inflammation, resulting in inflammation-related diseases. Therefore, we aimed to investigate the expression profile of NLRP3 in peripheral blood mononuclear cells isolated from patients with RA using immunocytochemical approaches in this study. Our findings demonstrated that NLRP3 expression was significantly increased in patients with RA in comparison with the healthy controls (p<0.01). The highest expression among the patient groups was observed in the active period cases and this expression considerably increased as compared to the control group (p<0.01). Patients in the remission period possessed the lowest expression among the patient groups. Since female gender is considered as an independent risk factor for the disease, the effect of gender on expressions was also investigated. NLRP3 was expressed at higher levels in female patients than in males; however, this difference was not significant (p>0.05). The expression patterns of the patient and control groups suggest that NLRP3 may be involved in the development and progression of the disease. To the best of our knowledge, our study is the first research investigating NLRP3 expression profile in peripheral blood mononuclear cells obtained from patients with RA using immunocytochemical approaches. The results of our study highlight significant aspects. However, further research using more sensitive methods with a larger number of cases is required to assess the function of NLRP3 in RA and to provide a deeper insight into the mechanism.

Anahtar Kelimeler

inflammasome, inflammation, NLRP3, rheumatoid arthritis

Destekleyen Kurum

Ankara University Scientific Research Projects

Proje Numarası

17L0237005

Kaynakça

• Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T. & Rouis, M. (2015). NLRP3 inflammasome: from a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology, 4, 296-307. doi:10.1016/j.redox.2015.01.008
• Ajeganova, S. & Huizinga, T. (2017). Sustained remission in rheumatoid arthritis: latest evidence and clinical considerations. Therapeutic Advances in Musculoskeletal Disease, 9(10), 249-262. doi:10.1177/1759720X17720366
• Aletaha, D., Ward, M. M., Machold, K. P., Nell, V. P., Stamm, T. & Smolen, J. S. (2005). Remission and active disease in rheumatoid arthritis: defining criteria for disease activity states. Arthritis & Rheumatism, 52(9), 2625-2636. doi:10.1002/ art.21235