Sıçanların Akciğer Dokusunda Metotreksat ile Oluşturulan Oksidatif Stres ve İnflamasyon Üzerine Klorojenik Asitin Etkisi

Yıl 2024, , 71 - 78, 01.10.2024

Ahmet Mentese , Selim Demir , Nihal Türkmen Alemdar , Elif Ayazoglu Demir , Yüksel Aliyazıcıoğlu

https://doi.org/10.59518/farabimedj.1504348

Öz

Metotreksat (MTX) yaygın olarak kullanılan bir kemoterapötik ajan olmasına rağmen, akciğer toksisitesi önemli bir sorun olmaya devam etmekte ve ilacın kullanımını sınırlamaktadır. MTX'e bağlı akciğer toksisitesinin moleküler mekanizması tam olarak anlaşılamamıştır. Ancak artan reaktif oksijen türlerinin neden olduğu oksidatif stres (OS) ve inflamasyon, akciğer hasarında önemli bir rol oynamaktadır. Klorojenik asit (CHA), son yıllarda OS ve inflamasyon ile ilişkili birçok patolojide yararlı etkilere sahip olduğu gösterilen doğal bir fenolik bileşiktir. Bu çalışma, MTX'e maruz bırakılan sıçanların akciğer dokusunda CHA'nın potansiyel terapötik etkilerinin ilk kez araştırılmasına odaklandı. Sıçanlarda ilk gün MTX (20 mg/kg) enjeksiyonu ile akciğer toksisitesi oluşturulduktan sonra, 3 gün boyunca iki farklı dozda CHA (1.5 ve 3 mg/kg) ile tedavi uygulandı. Sonuçlar, CHA tedavisinin, MTX'e maruz bırakılan sıçanlarda pulmoner lipid peroksidasyon, inflamasyon ve apoptoz seviyesini azalttığını ve pulmoner antioksidan sistemi desteklediğini gösterdi. Birlikte ele alındığında, CHA'nın antioksidan ve anti-inflamatuar özellikleri MTX'in neden olduğu akciğer hasarını hafifletmede merkezi bir rol oynayabilir, ancak kesin mekanizmanın daha kapsamlı klinik öncesi çalışmalarla araştırılması gerekmektedir.

Anahtar Kelimeler

Akciğer toksisitesi, Apoptoz, İnflamasyon, Klorojenik asit, Metotreksat, Oksidatif stres

Etik Beyan

Bu çalışma, Karadeniz Teknik Üniversitesi Hayvan Araştırmaları Etik Kurulu tarafından onaylanmış olup (Protokol no: 2023/08) hayvan deneylerinin in vivo deneylerle raporlanması (ARRIVE) kılavuzuna uygun olarak yürütülmüştür.

The Effect of Chlorogenic Acid on Methotrexate-Induced Oxidative Stress and Inflammation in Lung Tissue of Rats

Öz

Although methotrexate (MTX) is a widely used chemotherapeutic agent, lung toxicity remains a significant problem, limiting its use. The molecular mechanism of MTX-related lung toxicity is not fully understood. However, increased reactive oxygen species-induced oxidative stress (OS) and inflammation play an important role in lung injury. Chlorogenic acid (CHA) is a natural phenolic compound that has been shown in recent years to have beneficial effects in many pathologies associated with OS and inflammation. This study focused on investigating for the first time, the potential therapeutic effects of CHA in the lung tissue of rats exposed to MTX. After lung toxicity was induced in rats by MTX (20 mg/kg) injection on the first day, two different doses of CHA (1.5 and 3 mg/kg) were used for treatment for 3 days. The results showed that CHA treatment reduced the level of pulmonary lipid peroxidation, inflammation and apoptosis and promoted the pulmonary antioxidant system in rats subjected to MTX. Taken together, the antioxidant and anti-inflammatory properties of CHA may play a central role in attenuating MTX-induced lung injury, but the exact mechanism needs to be investigated in more extensive preclinical studies.

Anahtar Kelimeler

Apoptosis, Chlorogenic acid, Inflammation, Lung toxicity, Methotrexate, Oxidative stress

Etik Beyan

This study was approved by the Local Animal Research Ethics Committee of Karadeniz Technical University (Protocol no: 2023/08) and performed according to the animal research reporting of in vivo experiments (ARRIVE) guidelines.

Kaynakça

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