The Effects of Vasodilator Solutions on Apoptosis in Saphenous Vein with Mechanical Distention Application

Öz

Objective: This study explores the influence of vasodilators on the apoptotic activity related to endothelial damage caused by mechanical distension of the saphenous vein. Materials and Methods: Thirty-two patients without varicose veins undergoing coronary artery bypass graft operations were divided into four equal groups. Group I (control) Normal Saline, group II Papaverine group III Verapamil and group IV Nitroglycerine solutions. Percentange of immunohistochemical reactivity, pro-apoptotic bax and kaspaz-9 and anti-apoptotic bcl-2 were measured in the samples. Results: The percentage of cells which were positive for Bax (+) In Group I (Control) was 85.00 ± 6.89, Group II (Papaverine) 64.63 ± 8.73, Group III (Verapamil) 23.75 ± 4.20 and Group IV (Nitroglycerine) 22.00 ± 7.69. While there was no statistical difference between verepamil and nitroglycerin groups, they both had significantly decreased percentage of cells with proapoptotic Bax (+) compared to the control and the papaverine groups (p

Anahtar Kelimeler

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Mekanik Distansiyon Uygulanan Safen Vende Apopitozis Üzerine Vazodilatatör Solüsyonların Etkisi

Öz

Amaç: Bu çalışmamızda, safen vende mekanik distansiyonun sebep olduğu endotelyum harabiyetinde, apopitotik aktivitenin rolünü araştırmayı amaçladık. Gereç ve Yöntem: Çalışmaya her iki cinsten, koroner arter bypass greft operasyonuna alınan, venöz yetmezliği olmayan 32 hasta dahil edildi. Herbir grupta 8 hasta olmak üzere hastalar 4 gruba ayrıldı; Grup1 (Kontrol), Grup 2 (Papaverin), Grup 3 (Verapamil) ve Grup 4 (Nitrogliserin). Herbir grubun VSM'larından doku örnekleri alındı. Doku kesitlerinde immünohistokimyasal boyama ile proapopitotik bax, kaspaz-9 ve antiapopitotik bcl-2 boyanma yüzdeleri araştırıldı. Bulgular: Gruplarda yer alan safen venlerde proapopitotik Bax (+) hücre yüzdeleri sırasıyla 85.00 ± 6.89, 64.63 ± 8.73, 23.75 ± 4.20 ve 22.00 ± 7.69 olarak saptandı. Verapamil ve nitrogliserin grupları arasında istatistiksel bir fark bulunmazken, bu gruplardaki değerler kontrol ve papaverin grubuna göre anlamlı derecede düşük bulundu (p

Anahtar Kelimeler

• Apopitozis, Vazodilatatör, Safen ven

Kaynakça

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17. Gönderilme Tarihi: 28.02.2012