The Effect of Recombinant Il-11 on Retina in Experimental Ischemia/Reperfusion Model

Abstract

Objective: This experimental study was performed to investigate the protective effect of Recombinant IL-11 (rhIL-11) on retina tissue in the ischemia/reperfusion (I/R) injury in a guinea pig model. Materials and Methods: In this study, fifteen guinea pigs were randomly divided to three groups that each one has five guinea pigs: placebo, sham and the treatment (rhIL-11) groups. The guinea pigs in the placebo group were only given 0,1 cc saline solution intraperitoneally. Pressure induced 90 minutes of retinal ischemia and 48 hours of reperfusion were established in the sham and treatment groups. Saline for the sham group and 5μg/kg of rhIL-11 for the treatment group were administered intraperitoneally daily. At the end of the reperfusion period eyes of the animals of all groups were sacrificed and were enucleated. Retinal tissues were evaluated histopathologically. Results: The thickness of inner plexiform layer of retina in the treatment group was not shown significantly difference than placebo and sham (p=1.000, p= 0.095 respectively). However, in the sham group it was measured as significantly thicker than that of placebo group (p=0.032). The infiltration of the polymorphonuclear cells in the internal limiting membrane and inner plexiform layer of the sham group was significantly higher than those of the placebo (p=0.012) and treatment group (p=0.032). However, it was observed that there is no significant difference between treatment and placebo groups (p=0.189). Conclusion: rhIL-11 that shows a protective effect in retinal I/R injury kept retinal tissue in our study, this may make it a potential alternative agent for the prevention of retinal I/R injury.

Keywords

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Retinal İskemi-Reperfüzyon Modelinde Rekombinant İL-11'in Retinal Dokuya Etkisi

Öz

Amaç: Bu çalışma guinea pig modelinde retinal iskemi-reperfüzyon (İ-R) hasarında retina üzerine Rekombinant İL-11 (rhİL-11)'in koruyucu etkisinin araştırılması amacıyla yapılmıştır. Gereç ve Yöntem: Çalışmada 15 adet kobay randomize olarak her biri beş guinea pig içeren üç gruba ayrıldı: plasebo, sham ve tedavi (rhİL-11) grubu. Plasebo grubundaki kobaylara sadece günlük 0,1 cc salin solüsyonu intraperitoneal verildi. Her bir göze 90 dakika basınçla indüklenen iskemi peryodundan 1 saat önce ve 2 günlük reperfüzyon süreci boyunca sham grubundaki kobaylara günlük 0,1 cc salin solüsyonu ve tedavi grubundaki kobaylara ise günlük 5μg/kg rhİL-11 intraperitoneal olarak verildi. İkinci günün sonunda kobaylar sakrifiye edilerek tüm gruplardaki hayvanların her iki gözleri enüklee edilip retinaları histopatolojik olarak incelendi. Bulgular: Histopatolojik olarak incelenen retina kesitlerinde iç pleksiform tabakanın kalınlığı, rhİL-11 grubunda hem plasebo hem de sham grubuna göre anlamlı olarak farklı bulunmamıştır (sırasıyla p=1.000, p= 0.095). Ancak sham grubunda plasebo grubuna göre anlamlı olarak daha kalın ölçülmüştür (p=0.032). Retinanın iç tabakalarındaki (internal limitan membran ve iç pleksiform tabaka) polimorfonükleerlokosit (PMNL) infiltrasyonu, sham grubunda plasebo grubuna (p=0.012) ve rhİL-11 grubuna göre anlamlı artmış bulunurken (p=0.032), rhİL-11 grubunda plasebo grubuna göre anlamlı fark bulunamadı (p=0.189). Sonuç: Çalışmamızda retinal İ-R hasarında retinal dokuyu koruyucu etki gösteren rhİL-11, retinal İ-R hasarının engellenmesinde potansiyel alternatif bir ajan olabilir.

Anahtar Kelimeler

• Reperfüzyon hasarı, iskemi, rekombinant İL-11

Kaynakça

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27. Kabul Tarihi: 01.02.2009