Effects of Polychlorinated Biphenyls on Catecholaminergic Neurotransmitter Levels in Cortex, Striatum, Hippocampus and Hypothalamus of Rat

Abstract

Objectives: Polychlorinated biphenyls (PCBs) cause environmental chemical contamination and tend to bio-accumulate in living organisms due to their lipophylic properties. In this study, we have investigated effects two different Aroclor mixtures (Aroclor A1221 and A1254) on catecholamine levels in the cortex, striatum, hippocampus and hypothalamus in female rats. Material and Methods: Total 25 adult female Wistar rats were divided in three groups and subcutaneously injected with vehicle alone (4% dimethyl sulphoxide), A1221 (10 mg/kg) and A1254 (10 mg/kg) every other day for a period of 46 days. At the end of the experiments, animals were decapitated and brains rapidly removed. Cortex, striatum, hippocampus and hypothalamus regions were isolated, and homogenized. Brain noradrenalin, dopamine and metabolites concentrations were determined by HPLC-ECD method. Results: A1221 administration significantly increased levels of noradrenalin and its metabolite (dihydroxyphenyl glycol, DHPG) in cortex and striatum (p

Keywords

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Poliklorlu Bifenillerin Sıçan Korteks, Hipokampus, Striatum ve Hipotalamus Dokusunda Katekolaminerjik Nörotransmiter Düzeylerine Etkileri

Öz

Giriş: Poliklorlu bifeniller (PCB) endüstriyel amaçla üretilen organik klorlu bileşiklerdir. Doğada yaygın kimyasal kirlenmeye yol açtıkları ve lipofilik özellikleri nedeniyle canlı organizmada birikme eğilimi gösterdikleri bilinmektedir. Bu çalışmada, farklı iki PCB karışımı olan Aroklor 1221 (A1221) ve Aroklor 1254 (A1254)'ün dişi sıçanlarda korteks, hipokampus, striatum ve hipotalamus dokularında katekolamin düzeyleri üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Toplam 25 Wistar dişi sıçan üç gruba ayrıldı ve sırasıyla; taşıt solüsyonu olarak dimetil sülfoksit (%4), A1221 (10 mg/kg) ve A1254 (10 mg/kg) subkutan yolla gün aşırı olarak ve 46 gün süreyle uygulandı. Deneylerin sonunda hayvanlar dekapite edildi ve beyin dokuları hızla alındı. Korteks, hipotalamus, striatum ve hipokampus bölgeleri çıkartıldıktan sonra homojenize edildi. Doku noradrenalin, dopamin ve metabolit düzeyleri HPLC-ECD metodu kullanılarak ölçüldü. Bulgular: A1221; noradrenalin ve onun metaboliti olan dihidroksifenil glikol (DHPG) düzeylerini korteks ve striatumda artırırken (p

Anahtar Kelimeler

• PCB, Aroclor, noradrenalin, dopamin ve HPLC-ECD

Kaynakça

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45. Kabul Tarihi: 05.09.2006