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Infuse herbal oils: a comparative study of wheat germ and tomato seed oils

Yıl 2023, Cilt: 4 Sayı: 2, 99 - 104, 30.08.2023
https://doi.org/10.51753/flsrt.1313038

Öz

The use of traditional herbal medicine products has recently been revived, with special emphasis on the use of herbal oils in non-invasive wound treatment. Extraction conditions are very important in seed oils and especially suitable temperatures at which the extraction process is carried out. is vital for the preservation of primary and secondary metabolites. In this study, wheat germ (WG) was collected from the Central Anatolia Region and tomato seed (TS) was collected from the Aegean Region. Oils were obtained from these two seeds by cold pressing and their infused forms (WGO-TSO) were prepared. To investigate the in vitro activities of these study groups, cytotoxicity, wound healing, and antioxidant capacity tests were performed on HaCaT (Human keratinocyte) and Vero (Monkey kidney fibroblast-like) cell lines. According to the results of the intracellular cytotoxicity analysis, a common dose of 25 µg/mL was determined to be non-toxic for all groups, and this dose was then used as the working dose. Antioxidant capacity studies, in cells under oxidative stress with hydrogen peroxide, yielded positive results for all working groups and the WGO/TSO group showed statistically superior outcomes. In addition, similar results were obtained in wound healing experiments. As a result, using oils in the form of infusion may be more effective in combating oxidative stress and promoting wound healing than using oil alone.

Teşekkür

The author is grateful to EGE-LS company for providing cold-pressed seed oils.

Kaynakça

  • Ahmad, A., Husain, A., Mujeeb, M., Khan, S. A., Najmi, A. K., Siddique, N. A., Damanhouri, Z. A., & Anwar, F. (2013). A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5), 337-352.
  • Ajith, T., Usha, S., & Nivitha, V. (2007). Ascorbic acid and α-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clinica Chimica Acta, 375(1-2), 82-86.
  • Allaw, M., Manconi, M., Caboni, P., Bacchetta, G., Escribano-Ferrer, E., Peris, J. E., Nacher, A., Diez-Sales, O., & Manca, M. L. (2021). Formulation of liposomes loading lentisk oil to ameliorate topical delivery, attenuate oxidative stress damage and improve cell migration in scratch assay. Biomedicine and Pharmacotherapy, 144, 112351.
  • Barlas, F., Celikbas, E., Arslan, M., Timur, S., & Yagci, Y. (2019). Gold nanoparticle conjugated poly (p‐phenylene‐β‐cyclodextrin)‐graft‐poly (ethylene glycol) for theranostic applications. Journal of Applied Polymer Science, 136(12), 47250.
  • Barnes, P. (1982). Lipid composition of wheat germ and wheat germ oil. Fette, Seifen, Anstrichmittel, 84(7), 256-269.
  • Bengi V, U., Saygun, I., Bal, V., Ozcan, E., Kose Ozkan, C., Torun, D., Avcu, F., & Kantarcı, A. (2022). Effect of antioxidant lycopene on human osteoblasts. Clinical Oral Investigations, 1-7.
  • Binsuwaidan, R., Sultan, A. A., Negm, W. A., Attallah, N. G., Alqahtani, M. J., Hussein, I. A., Shaldam, M. A., El-Sherbeni, S. A., & Elekhnawy, E. (2022). Bilosomes as nanoplatform for oral delivery and modulated in vivo antimicrobial activity of lycopene. Pharmaceuticals, 15(9), 1043.
  • Budala, D. G., Martu, M. A., Maftei, G. A., Diaconu-Popa, D. A., Danila, V., & Luchian, I. (2023). The role of natural compounds in optimizing contemporary dental treatment-current status and future trends. Journal of Functional Biomaterials, 14(5), 273.
  • Catzeddu, P., Fois, S., Tolu, V., Sanna, M., Braca, A., Vitangeli, I., ... & Roggio, T. (2023). Quality evaluation of fresh pasta fortified with sourdough containing wheat germ and wholemeal semolina. Foods, 12(14), 2641.
  • Ehterami, A., Salehi, M., Farzamfar, S., Samadian, H., Vaez, A., Ghorbani, S., Ai, J., & Sahrapeyma, H. (2019). Chitosan/alginate hydrogels containing Alpha-tocopherol for wound healing in rat model. Journal of Drug Delivery Science and Technology, 51, 204-213.
  • El-Marasy, S. A., El-Shenawy, S. M., El-Khatib, A. S., El-Shabrawy, O. A., & Kenawy, S. A. (2012). Effect of Nigella sativa and wheat germ oils on scopolamine-induced memory impairment in rats. Bulletin of Faculty of Pharmacy, Cairo University, 50(2), 81-88.
  • Eller, F., Moser, J., Kenar, J., & Taylor, S. (2010). Extraction and analysis of tomato seed oil. Journal of the American Oil Chemists' Society, 87, 755-762.
  • Ghafoor, K., Özcan, M. M., AL‐Juhaımı, F., Babıker, E. E., Sarker, Z. I., Ahmed, I. A. M., & Ahmed, M. A. (2017). Nutritional composition, extraction, and utilization of wheat germ oil: A review. European Journal of Lipid Science and Technology, 119(7), 1600160.
  • Guler, E., Barlas, F. B., Yavuz, M., Demir, B., Gumus, Z. P., Baspinar, Y., Coskunol, H., & Timur, S. (2014). Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations. Colloids and Surfaces B: Biointerfaces, 121, 299-306.
  • Gumus, Z. P., Argon, Z. U., Celenk, V. U., & Timur, S. (2020). Cold pressed tomato (Lycopersicon esculentum L.) seed oil. In: Ramadan M. F. (ed) Cold pressed oils (pp. 449-458). Elsevier.
  • Guven, H., Durmus, N., Hocaoglu, N., Guner, O., Acar, S., Akan, P., & Calan, O. G. (2022). Protective effects of wheat germ oil against erectile and endothelial dysfunction in streptozotocin-induced diabetic rats. International Journal of Impotence Research, 34(6), 581-587.
  • Harrabi, S., Ferchichi, A., Fellah, H., Feki, M., & Hosseinian, F. (2021). Chemical composition and in vitro anti-inflammatory activity of wheat germ oil depending on the extraction procedure. Journal of Oleo Science, 70(8), 1051-1058.
  • Hung, C.-F., Huang, T.-F., Chen, B.-H., Shieh, J.-M., Wu, P.-H., & Wu, W.-B. (2008). Lycopene inhibits TNF-α-induced endothelial ICAM-1 expression and monocyte-endothelial adhesion. European Journal of Pharmacology, 586(1-3), 275-282.
  • Khan, Z. A., Prabhu, N., Ahmed, N., Lal, A., Issrani, R., Maqsood, A., Vohra, F., & Alam, M. K. (2022). A comparative study on alvogyl and a mixture of black seed oil and powder for alveolar osteitis: a randomized double-blind controlled clinical trial. International Journal of Clinical Practice, 2022.
  • Kumar, M., Sharma, D., & Singh, V. P. (2023). Modulation of the chain-breaking antioxidant activity of phenolic organochalcogens with various co-antioxidants at various pH values. Organic & Biomolecular Chemistry, 21(6), 1316-1327.
  • Lee, C. H., An, D. S., Lee, S. C., Park, H. J., & Lee, D. S. (2004). A coating for use as an antimicrobial and antioxidative packaging material incorporating nisin and α-tocopherol. Journal of Food Engineering, 62(4), 323-329.
  • Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333.
  • Liu, R., Xu, Y., Chang, M., Tang, L., Lu, M., Liu, R., Jin, Q., & Wang, X. (2021). Antioxidant interaction of α-tocopherol, γ-oryzanol and phytosterol in rice bran oil. Food Chemistry, 343, 128431.
  • Machmudah, S., Winardi, S., Sasaki, M., Goto, M., Kusumoto, N., & Hayakawa, K. (2012). Lycopene extraction from tomato peel by-product containing tomato seed using supercritical carbon dioxide. Journal of Food Engineering, 108(2), 290-296.
  • Małecka, M. (2002). Antioxidant properties of the unsaponifiable matter isolated from tomato seeds, oat grains and wheat germ oil. Food Chemistry, 79(3), 327-330.
  • Munuswamy, H., Thirunavukkarasu, T., Rajamani, S., Elumalai, E. K., & Ernest, D. (2013). A review on antimicrobial efficacy of some traditional medicinal plants in Tamilnadu. Journal of Acute Disease, 2(2), 99-105.
  • Przybylska, S. (2020). Lycopene–a bioactive carotenoid offering multiple health benefits: a review. International Journal of Food Science & Technology, 55(1), 11-32.
  • Przybylska, S., & Tokarczyk, G. (2022). Lycopene in the prevention of cardiovascular diseases. International Journal of Molecular Sciences, 23(4), 1957.
  • Razzaq, L., Abbas, M. M., Miran, S., Asghar, S., Nawaz, S., Soudagar, M. E. M., Shaukat, N., Veza, I., Khalil, S., & Abdelrahman, A. (2022). Response surface methodology and artificial neural networks-based yield optimization of biodiesel sourced from mixture of palm and cotton seed oil. Sustainability, 14(10), 6130.
  • Roy, I., Magesh, K. T., Sathyakumar, M., Sivachandran, A., Purushothaman, D., & Aravindhan, R. (2023). Evaluation of wound healing property of the ethanolic extract of glycyrrhiza glabra on vero cell lines using in vitro scratch assay test. Journal of Pharmacy and Bioallied Sciences, 15(Suppl 1), S630-S635.
  • Osma, E., Ozyigit, I. I., Leblebici, Z., Demir, G., & Serin, M. (2012). Determination of heavy metal concentrations in tomato (Lycopersicon esculentum Miller) grown in different station types. Romanian Biotechnological Letters, 17(1), 6962-6974.
  • Sumara, A., Stachniuk, A., Trzpil, A., Bartoszek, A., Montowska, M., & Fornal, E. (2023). LC-MS metabolomic profiling of five types of unrefined, cold-pressed seed oils to identify markers to determine oil authenticity and to test for oil adulteration. Molecules, 28(12), 4754.
  • Shakir, S. K., Irfan, S., Akhtar, B., Rehman, S. U., Daud, M. K., Taimur, N., & Azizullah, A. (2018). Pesticide-induced oxidative stress and antioxidant responses in tomato (Solanum lycopersicum) seedlings. Ecotoxicology, 27, 919-935.
  • Shankar, K., & Liao, L. P. (2004). Traditional systems of medicine. Physical Medicine and Rehabilitation Clinics, 15(4), 725-747.
  • Yuenyong, J., Pokkanta, P., Phuangsaijai, N., Kittiwachana, S., Mahatheeranont, S., & Sookwong, P. (2021). GC-MS and HPLC-DAD analysis of fatty acid profile and functional phytochemicals in fifty cold-pressed plant oils in Thailand. Heliyon, 7(2), e06304.
  • Zahid, S., Khalid, H., Ikram, F., Iqbal, H., Samie, M., Shahzadi, L., Shah, A. T., Yar, M., Chaudhry, A. A., & Awan, S. J. (2019). Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration. Materials Science and Engineering: C, 101, 438-447.
  • Zaid, A. N., & Al Ramahi, R. (2019). Depigmentation and anti-aging treatment by natural molecules. Current Pharmaceutical Design, 25(20), 2292-2312.
Yıl 2023, Cilt: 4 Sayı: 2, 99 - 104, 30.08.2023
https://doi.org/10.51753/flsrt.1313038

Öz

Kaynakça

  • Ahmad, A., Husain, A., Mujeeb, M., Khan, S. A., Najmi, A. K., Siddique, N. A., Damanhouri, Z. A., & Anwar, F. (2013). A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5), 337-352.
  • Ajith, T., Usha, S., & Nivitha, V. (2007). Ascorbic acid and α-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clinica Chimica Acta, 375(1-2), 82-86.
  • Allaw, M., Manconi, M., Caboni, P., Bacchetta, G., Escribano-Ferrer, E., Peris, J. E., Nacher, A., Diez-Sales, O., & Manca, M. L. (2021). Formulation of liposomes loading lentisk oil to ameliorate topical delivery, attenuate oxidative stress damage and improve cell migration in scratch assay. Biomedicine and Pharmacotherapy, 144, 112351.
  • Barlas, F., Celikbas, E., Arslan, M., Timur, S., & Yagci, Y. (2019). Gold nanoparticle conjugated poly (p‐phenylene‐β‐cyclodextrin)‐graft‐poly (ethylene glycol) for theranostic applications. Journal of Applied Polymer Science, 136(12), 47250.
  • Barnes, P. (1982). Lipid composition of wheat germ and wheat germ oil. Fette, Seifen, Anstrichmittel, 84(7), 256-269.
  • Bengi V, U., Saygun, I., Bal, V., Ozcan, E., Kose Ozkan, C., Torun, D., Avcu, F., & Kantarcı, A. (2022). Effect of antioxidant lycopene on human osteoblasts. Clinical Oral Investigations, 1-7.
  • Binsuwaidan, R., Sultan, A. A., Negm, W. A., Attallah, N. G., Alqahtani, M. J., Hussein, I. A., Shaldam, M. A., El-Sherbeni, S. A., & Elekhnawy, E. (2022). Bilosomes as nanoplatform for oral delivery and modulated in vivo antimicrobial activity of lycopene. Pharmaceuticals, 15(9), 1043.
  • Budala, D. G., Martu, M. A., Maftei, G. A., Diaconu-Popa, D. A., Danila, V., & Luchian, I. (2023). The role of natural compounds in optimizing contemporary dental treatment-current status and future trends. Journal of Functional Biomaterials, 14(5), 273.
  • Catzeddu, P., Fois, S., Tolu, V., Sanna, M., Braca, A., Vitangeli, I., ... & Roggio, T. (2023). Quality evaluation of fresh pasta fortified with sourdough containing wheat germ and wholemeal semolina. Foods, 12(14), 2641.
  • Ehterami, A., Salehi, M., Farzamfar, S., Samadian, H., Vaez, A., Ghorbani, S., Ai, J., & Sahrapeyma, H. (2019). Chitosan/alginate hydrogels containing Alpha-tocopherol for wound healing in rat model. Journal of Drug Delivery Science and Technology, 51, 204-213.
  • El-Marasy, S. A., El-Shenawy, S. M., El-Khatib, A. S., El-Shabrawy, O. A., & Kenawy, S. A. (2012). Effect of Nigella sativa and wheat germ oils on scopolamine-induced memory impairment in rats. Bulletin of Faculty of Pharmacy, Cairo University, 50(2), 81-88.
  • Eller, F., Moser, J., Kenar, J., & Taylor, S. (2010). Extraction and analysis of tomato seed oil. Journal of the American Oil Chemists' Society, 87, 755-762.
  • Ghafoor, K., Özcan, M. M., AL‐Juhaımı, F., Babıker, E. E., Sarker, Z. I., Ahmed, I. A. M., & Ahmed, M. A. (2017). Nutritional composition, extraction, and utilization of wheat germ oil: A review. European Journal of Lipid Science and Technology, 119(7), 1600160.
  • Guler, E., Barlas, F. B., Yavuz, M., Demir, B., Gumus, Z. P., Baspinar, Y., Coskunol, H., & Timur, S. (2014). Bio-active nanoemulsions enriched with gold nanoparticle, marigold extracts and lipoic acid: In vitro investigations. Colloids and Surfaces B: Biointerfaces, 121, 299-306.
  • Gumus, Z. P., Argon, Z. U., Celenk, V. U., & Timur, S. (2020). Cold pressed tomato (Lycopersicon esculentum L.) seed oil. In: Ramadan M. F. (ed) Cold pressed oils (pp. 449-458). Elsevier.
  • Guven, H., Durmus, N., Hocaoglu, N., Guner, O., Acar, S., Akan, P., & Calan, O. G. (2022). Protective effects of wheat germ oil against erectile and endothelial dysfunction in streptozotocin-induced diabetic rats. International Journal of Impotence Research, 34(6), 581-587.
  • Harrabi, S., Ferchichi, A., Fellah, H., Feki, M., & Hosseinian, F. (2021). Chemical composition and in vitro anti-inflammatory activity of wheat germ oil depending on the extraction procedure. Journal of Oleo Science, 70(8), 1051-1058.
  • Hung, C.-F., Huang, T.-F., Chen, B.-H., Shieh, J.-M., Wu, P.-H., & Wu, W.-B. (2008). Lycopene inhibits TNF-α-induced endothelial ICAM-1 expression and monocyte-endothelial adhesion. European Journal of Pharmacology, 586(1-3), 275-282.
  • Khan, Z. A., Prabhu, N., Ahmed, N., Lal, A., Issrani, R., Maqsood, A., Vohra, F., & Alam, M. K. (2022). A comparative study on alvogyl and a mixture of black seed oil and powder for alveolar osteitis: a randomized double-blind controlled clinical trial. International Journal of Clinical Practice, 2022.
  • Kumar, M., Sharma, D., & Singh, V. P. (2023). Modulation of the chain-breaking antioxidant activity of phenolic organochalcogens with various co-antioxidants at various pH values. Organic & Biomolecular Chemistry, 21(6), 1316-1327.
  • Lee, C. H., An, D. S., Lee, S. C., Park, H. J., & Lee, D. S. (2004). A coating for use as an antimicrobial and antioxidative packaging material incorporating nisin and α-tocopherol. Journal of Food Engineering, 62(4), 323-329.
  • Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333.
  • Liu, R., Xu, Y., Chang, M., Tang, L., Lu, M., Liu, R., Jin, Q., & Wang, X. (2021). Antioxidant interaction of α-tocopherol, γ-oryzanol and phytosterol in rice bran oil. Food Chemistry, 343, 128431.
  • Machmudah, S., Winardi, S., Sasaki, M., Goto, M., Kusumoto, N., & Hayakawa, K. (2012). Lycopene extraction from tomato peel by-product containing tomato seed using supercritical carbon dioxide. Journal of Food Engineering, 108(2), 290-296.
  • Małecka, M. (2002). Antioxidant properties of the unsaponifiable matter isolated from tomato seeds, oat grains and wheat germ oil. Food Chemistry, 79(3), 327-330.
  • Munuswamy, H., Thirunavukkarasu, T., Rajamani, S., Elumalai, E. K., & Ernest, D. (2013). A review on antimicrobial efficacy of some traditional medicinal plants in Tamilnadu. Journal of Acute Disease, 2(2), 99-105.
  • Przybylska, S. (2020). Lycopene–a bioactive carotenoid offering multiple health benefits: a review. International Journal of Food Science & Technology, 55(1), 11-32.
  • Przybylska, S., & Tokarczyk, G. (2022). Lycopene in the prevention of cardiovascular diseases. International Journal of Molecular Sciences, 23(4), 1957.
  • Razzaq, L., Abbas, M. M., Miran, S., Asghar, S., Nawaz, S., Soudagar, M. E. M., Shaukat, N., Veza, I., Khalil, S., & Abdelrahman, A. (2022). Response surface methodology and artificial neural networks-based yield optimization of biodiesel sourced from mixture of palm and cotton seed oil. Sustainability, 14(10), 6130.
  • Roy, I., Magesh, K. T., Sathyakumar, M., Sivachandran, A., Purushothaman, D., & Aravindhan, R. (2023). Evaluation of wound healing property of the ethanolic extract of glycyrrhiza glabra on vero cell lines using in vitro scratch assay test. Journal of Pharmacy and Bioallied Sciences, 15(Suppl 1), S630-S635.
  • Osma, E., Ozyigit, I. I., Leblebici, Z., Demir, G., & Serin, M. (2012). Determination of heavy metal concentrations in tomato (Lycopersicon esculentum Miller) grown in different station types. Romanian Biotechnological Letters, 17(1), 6962-6974.
  • Sumara, A., Stachniuk, A., Trzpil, A., Bartoszek, A., Montowska, M., & Fornal, E. (2023). LC-MS metabolomic profiling of five types of unrefined, cold-pressed seed oils to identify markers to determine oil authenticity and to test for oil adulteration. Molecules, 28(12), 4754.
  • Shakir, S. K., Irfan, S., Akhtar, B., Rehman, S. U., Daud, M. K., Taimur, N., & Azizullah, A. (2018). Pesticide-induced oxidative stress and antioxidant responses in tomato (Solanum lycopersicum) seedlings. Ecotoxicology, 27, 919-935.
  • Shankar, K., & Liao, L. P. (2004). Traditional systems of medicine. Physical Medicine and Rehabilitation Clinics, 15(4), 725-747.
  • Yuenyong, J., Pokkanta, P., Phuangsaijai, N., Kittiwachana, S., Mahatheeranont, S., & Sookwong, P. (2021). GC-MS and HPLC-DAD analysis of fatty acid profile and functional phytochemicals in fifty cold-pressed plant oils in Thailand. Heliyon, 7(2), e06304.
  • Zahid, S., Khalid, H., Ikram, F., Iqbal, H., Samie, M., Shahzadi, L., Shah, A. T., Yar, M., Chaudhry, A. A., & Awan, S. J. (2019). Bi-layered α-tocopherol acetate loaded membranes for potential wound healing and skin regeneration. Materials Science and Engineering: C, 101, 438-447.
  • Zaid, A. N., & Al Ramahi, R. (2019). Depigmentation and anti-aging treatment by natural molecules. Current Pharmaceutical Design, 25(20), 2292-2312.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Hücre Gelişimi, Proliferasyon ve Ölümü
Bölüm Araştırma Makaleleri
Yazarlar

Fırat Barlas 0000-0001-6401-686X

Yayımlanma Tarihi 30 Ağustos 2023
Gönderilme Tarihi 11 Haziran 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 4 Sayı: 2

Kaynak Göster

APA Barlas, F. (2023). Infuse herbal oils: a comparative study of wheat germ and tomato seed oils. Frontiers in Life Sciences and Related Technologies, 4(2), 99-104. https://doi.org/10.51753/flsrt.1313038

Creative Commons License

Frontiers in Life Sciences and Related Technologies is licensed under a Creative Commons Attribution 4.0 International License.