OKSİDATİF STRESE MARUZ BIRAKILMIŞ RATLARA VERİLEN BİOCHANİN A‘NIN SERUMDAKİ BAZI BİYOKİMYASAL PARAMETRELER ÜZERİNE ETKİSİ

Yıl 2008, Cilt: 6 Sayı: 2, 1 - 5, 30.03.2008

Ahmet Özkaya

Öz

İzoflavonlar
birçok bitkide bulunan fitokimyasallardır. Biochanin A 5,7 – dihidroksi 4’-
metoksi izoflavon-dur. İzoflavonlar antioksidan özellik gösterir. Bu
araştırmada, H₂O₂ ileoksidatif strese maruz
bırakılan sıçanlar üzerine gavaj yöntemiyle verilen Biochanin A’nın koruyucu
etkileri araştırıldı. Çalışmada MDA, enzimler (katalaz, glutatyon peroksidaz),
GSH düzeyleri ölçüldü. Bu amaçla Biochanin A, H₂O₂,
Kontrol ve H₂O₂+ Biochanin A grupları kullanıldı.

 

Deneylerden
elde edilen sonuçlara göre, H₂O₂ MDA düzeyleri kontrol
grubuna göre arttı (p<0.0001). Biocha-nin A’nın etkisiyle H₂O₂+
Biochanin A grubu MDA düzeyi H₂O₂ grubuna göre azaldı
(p<0.001). Tüm grupların katalaz ve glutatyon peroksidaz enzim aktiviteleri
kontrol grubuna göre artmıştır (p<0.0001). Sonuçlarımız, Biochanin A
antioksidan özellikte bir madde olduğunu göstermektedir.

Anahtar Kelimeler

H2O2, MDA, Katalaz, Glutatyon Peroksidaz, GSH, Biochanin A, Serum, Sıçan

Kaynakça

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