Evaluation of Candida albicans biofilm formation on different soft lining materials

Öz

OBJECTIVE: The purpose of this study was to evaluate Candida albicans biofilm formation on soft liners prepared against two surfaces with different properties.

MATERIALS AND METHOD: Four soft liners (Molloplast B, Permafleks, Elite-soft, Ufi Gel P) were prepared against glass or dental plaster surfaces (5x5x2 mm; n=5). The surface roughness and contact angle of the soft liner specimens were measured. Candida albicans ATCC 10231 was grown in Sabouraud dextrose broth (SDB) at 37 °C for 24 h. For biofilm formation, the specimens were incubated in polystrene wells together with fresh SDB and the C. albicans culture at 37 °C for 48 h. The specimens were stained with 0.1% crystal violet and dehydrated at 65 °C. The quantification of the C. albicans biofilm formation was performed spectrophotometrically. Comparison of the surface roughness, contact angle and biofilm formation between the materials was done using one way ANOVA and Tukey tests. Correlation between surface roughness, contact angle and biofilm formation on the specimens prepared against glass or plaster surfaces was performed using Pearson correlation analysis.

RESULTS: Specimens prepared against plaster showed significantly higher surface roughness, contact angle and biofilm formation values in comparison to those prepared against glass surface (p<0.05). Among the specimens prepared against the dental plaster surface, the highest and the lowest biofilm formation values were observed in the Ufi Gel P and Molloplast B specimens, respectively. Among the specimens prepared against the glass surface, the greatest biofilm formation was shown for Ufi Gel P. There was no correlation between the surface properties (surface roughness and contact angle) and the amount of biofilm formation for the soft liner materials tested in this study (p>0.05).

CONCLUSION: Although the soft liners tested in this study had similar surface topographic properties, they showed different amounts of C. albicans biofilm formation. Smooth surfaces were found to reduce the biofilm formation.

Anahtar Kelimeler

• Biofilm,Candida albicans,Polydimethylsiloxanes

Farklı yumuşak astar materyallerinin Candida albicans biyofilm formasyonu açısından değerlendirilmesi

Öz

AMAÇ: Bu çalışmada iki farklı yüzey yapısına göre hazırlanan yumuşak astar materyallerinde Candida albicans biyofilm oluşumunun değerlendirilmesi amaçlanmıştır. GEREÇ VE YÖNTEM: Dört yumuşak astar materyali (Molloplast B, Permafleks, Elite-Soft, Ufi Gel P) cam veya alçı yüzeyler üzerinde hazırlandı (5x5x2 mm; n=5). Tüm örneklerin yüzey pürüzlülükleri ve temas açıları ölçüldü. Candida albicans ATCC 10231 Sabouraud dextrose besiyeri (SDB) içerisinde 37 °C’de 24 saat süresince çoğaltıldı. Ardından, biyofilm oluşumu için örnekler polistren kuyucuklarda, önceden elde edilen Candida albicans kültürü ve taze SDB ilavesiyle 37 °C’de 48 saat süresince enkübe edildi. Örnekler %0.1 kristal viyole solüsyonu ile boyanarak 65 °C’de kurumaya bırakıldı. Biyofilm oluşumu spektrofotometrik yöntemle incelendi. Test edilen materyallerin yüzey pürüzlülüğü, temas açıları ve biyofilm formasyonlarının karşılaştırılması oneway ANOVA ve Tukey testi ile yapıldı. Cam veya alçı yüzeylerde hazırlanan örneklerin yüzey pürüzlülüğü, temas açıları ve biyofilm oluşumu arasındaki korelasyon Pearson korelasyon analizi ile incelendi. BULGULAR: Alçı yüzeyinde hazırlanan örneklerde yüzey pürüzlülüğü, temas açısı ve biyofilm oluşumu, cam üzerinde hazırlananlara göre istatistiksel olarak daha fazla bulundu (p 0.05). SONUÇ: Test edilen yumuşak astar materyalleri, yüzey topografik özellikleri açısından benzer olsalar da farklı miktarlarda Candida albicans biyofilm oluşumu gösterdiler. Düz yüzeylerin biyofilm oluşumunu azalttığı gözlendi.

Anahtar Kelimeler

• Biyofilm, Kandida albikans, Polidimetilsiloksanlar

Kaynakça

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