Evaluation of Candida albicans biofilm formation on different soft lining materials

Yıl 2015, Cilt: 32 Sayı: 1, 19 - 25, 09.02.2015

Duygu Karakış Canan Akay Demet Erdönmez Arife Doğan

https://doi.org/10.17214/aot.50404

Cited By: 1

Öz

OBJECTIVE: The purpose of this study was to evaluate Candida albicans biofilm formation on soft liners prepared against two surfaces with different properties.

MATERIALS AND METHOD: Four soft liners (Molloplast B, Permafleks, Elite-soft, Ufi Gel P) were prepared against glass or dental plaster surfaces (5x5x2 mm; n=5). The surface roughness and contact angle of the soft liner specimens were measured. Candida albicans ATCC 10231 was grown in Sabouraud dextrose broth (SDB) at 37 °C for 24 h. For biofilm formation, the specimens were incubated in polystrene wells together with fresh SDB and the C. albicans culture at 37 °C for 48 h. The specimens were stained with 0.1% crystal violet and dehydrated at 65 °C. The quantification of the C. albicans biofilm formation was performed spectrophotometrically. Comparison of the surface roughness, contact angle and biofilm formation between the materials was done using one way ANOVA and Tukey tests. Correlation between surface roughness, contact angle and biofilm formation on the specimens prepared against glass or plaster surfaces was performed using Pearson correlation analysis.

RESULTS: Specimens prepared against plaster showed significantly higher surface roughness, contact angle and biofilm formation values in comparison to those prepared against glass surface (p<0.05). Among the specimens prepared against the dental plaster surface, the highest and the lowest biofilm formation values were observed in the Ufi Gel P and Molloplast B specimens, respectively. Among the specimens prepared against the glass surface, the greatest biofilm formation was shown for Ufi Gel P. There was no correlation between the surface properties (surface roughness and contact angle) and the amount of biofilm formation for the soft liner materials tested in this study (p>0.05).

CONCLUSION: Although the soft liners tested in this study had similar surface topographic properties, they showed different amounts of C. albicans biofilm formation. Smooth surfaces were found to reduce the biofilm formation.

Anahtar Kelimeler

Biofilm, Candida albicans, Polydimethylsiloxanes

Farklı yumuşak astar materyallerinin Candida albicans biyofilm formasyonu açısından değerlendirilmesi

Öz

AMAÇ: Bu çalışmada iki farklı yüzey yapısına göre
hazırlanan yumuşak astar materyallerinde Candida albicans
biyofilm oluşumunun değerlendirilmesi amaçlanmıştır.
GEREÇ VE YÖNTEM: Dört yumuşak astar materyali (Molloplast
B, Permafleks, Elite-Soft, Ufi Gel P) cam veya alçı yüzeyler
üzerinde hazırlandı (5x5x2 mm; n=5). Tüm örneklerin yüzey
pürüzlülükleri ve temas açıları ölçüldü. Candida albicans
ATCC 10231 Sabouraud dextrose besiyeri (SDB) içerisinde
37 °C’de 24 saat süresince çoğaltıldı. Ardından, biyofilm
oluşumu için örnekler polistren kuyucuklarda, önceden
elde edilen Candida albicans kültürü ve taze SDB ilavesiyle
37 °C’de 48 saat süresince enkübe edildi. Örnekler %0.1
kristal viyole solüsyonu ile boyanarak 65 °C’de kurumaya
bırakıldı. Biyofilm oluşumu spektrofotometrik yöntemle incelendi.
Test edilen materyallerin yüzey pürüzlülüğü, temas
açıları ve biyofilm formasyonlarının karşılaştırılması oneway
ANOVA ve Tukey testi ile yapıldı. Cam veya alçı yüzeylerde hazırlanan örneklerin yüzey pürüzlülüğü, temas
açıları ve biyofilm oluşumu arasındaki korelasyon Pearson
korelasyon analizi ile incelendi.
BULGULAR: Alçı yüzeyinde hazırlanan örneklerde yüzey
pürüzlülüğü, temas açısı ve biyofilm oluşumu, cam üzerinde
hazırlananlara göre istatistiksel olarak daha fazla
bulundu (p 0.05).
SONUÇ: Test edilen yumuşak astar materyalleri, yüzey topografik
özellikleri açısından benzer olsalar da farklı miktarlarda
Candida albicans biyofilm oluşumu gösterdiler.
Düz yüzeylerin biyofilm oluşumunu azalttığı gözlendi.

Anahtar Kelimeler

Biyofilm, Kandida albikans, Polidimetilsiloksanlar

Kaynakça

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