De-etiolasyon Sürecinde Fasulye (Phaseolus vulgaris L.) Yapraklarında Antioksidan Enzim Aktiviteleri ve Toplam Fenolik Bileşik Miktarlarında Değişimler

Öz

Bu çalışmada, de-etiyole fasulye fidelerinde antioksidan enzim aktiviteleri, hidrojen peroksit, malondialdehit ve total fenolik madde miktarları araştırılmıştır. De-etiyole fasulye yapraklarında katalaz (CAT), peroksidaz (POD) ve askorbat peroksidaz (APX) aktiviteleri kontrol (ışık) grubuyla karşılaştırıldığında kısmi olarak yüksek olduğu görülmüştür. De-etiyole fasulye bitkisinde lipid peroksidasyonu kontrol grubundan önemli oranda yüksek olmasına rağmen hidrojen peroksit miktarında önemli bir fark yoktur. Total fenolik bileşikler kontrol ve de-etiyole bitkilerde yüksek oranda gözlenirken etiyole fidelerde belirlenememiştir. Karanlık-ışık geçişlerinde oksidatif hasar azalırken total fenolik bileşik miktarında artış meydana gelmiştir. 

Anahtar Kelimeler

• De-etiyole,fasulye,hidrojen peroksit,lipid peroksidasyonu

Kaynakça

1. Angelini, R., Manes, F., Federico, R., 1990. Spatial and functional correlation between daimine-oxidase and peroxidase activities and their dependence upon deetiolation and wounding in chick-pea stems. Planta. 182, 89-96.
2. Asard, H and Bérczi, A., 1998. Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings. Protoplasma. 205, 37-42.
3. Bartolomé, B., Estrella, I., Hernándes, T., 1997. Changes in phenolic compounds in lentils (Lens cullinaris) during germination and fermantation. Z Lebensm Unters Forsch A. 205, 290- 294.
4. Demir, H., Öztürk, L., Kısa, D., Elmastaş M., 2017. De-etiyole Fasulye fidelerinin kök ve yapraklarında yağ asidi kompozisyonu ve peroksidasyonu. Gaziosmanpaşa Bilimsel Araştırma Dergisi. 6(2), 54-61.
5. Duncan, B.D., 1955. Multiple range and multiple F-tests. Biometrics. p. 1- 42.
6. Gechev T., Willekens H., Montagu MV., Inzé D., Camp WV., Toneva V., Minkov I., 2003. Different responses of tobacco antioxidant enzymes to light and chilling stress. J. Plant. Physiol. 160, 509-515.
7. Goupy, P.M., Varoquauaz, P.J.A., Nicolas, J.J., Macheix, J.J., 1990. Identification and localization of hidroxycinnamoyl and flavonol derivatives from endive (Cichorium endivia L. cv. Geante Maraichere). J. Agric. Food Chem. 38, 2116- 2121.
8. Hajlaoui, H., Denden, M., El Ayeb, N., 2009. Changes in fatty acids composition, hydrogen peroxide generation and lipid peroxidation of salt-stressed corn (Zea mays L.) roots. Acta Physiol Plant. 31, 787-796.

9. Havir, E.A., Mchale, N.A., 1987. Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves. Plant Physiol. 84, 450-455.
10. Horbowicz, M., Wiczkowski W., Szawara-Nowak D., Sawicki T., Kosson R., Sytykiewicz., 2015. The level of flavonoids and amines in de-etiolated and methyl jasmonate treated seedlings of common buckwheat. Phytochemistry Letters. 13, 15-19.
11. Mackerness, SAH., Jordan, BR and Thomas B., 1999. Reactive oxygen species in the regulation of photosynthetic genes by ultraviolet- B radiation (UV-B: 280-320nm) in green and etiolated buds of pea (Pisum sativum L.). Journal of Photochemistry and Photobiology B: Biology. 48, 180-188.
12. Luhová, L., Lebeda, A., Hedererova, D., Pec, P. 2003. Activites of amine oxidase, peroxidase and catalase in seedlings of Pisum sativum L. under different light conditions. Plant Soil Environ. 49 (4), 151-157.
13. Maricle, B.R., 2010. Changes in chlrophyll content and antioxidant capacity during dark to light transitions in etiolated seedlings: Comparisons of species and units of enzyme activity. Transactions of the Kansas Academy of Science. 113(3-4), 177-190.
14. Parida, A.K., Das, A.B., 2005. Salt tolerance and salinity effects on plants a review. Ecotoxicology and Environmental Safety. 60, 324- 349.
15. Pennycooke, J.C., Cox, S., Stushnoff, C., 2005. Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia hybrida). Environmental and Experimental Botany. 53, 225–232.
16. Symons, G.M ve Reid, J.B., 2003. Interactions between light and plant hormones during de-etilation. J. Plant Growth Regulation. 22, 3-14.
17. Sivaci, A., Sökmen, M., Günes, T., 2007. Biochemical changes in green and etiolated stems of MM106 apple rootstock. Asian Journal of Plant Sciences. 6(5), 839- 843.
18. Taiz,L. ve Zeiger, E. 2008. Bitki Fizyolojisi, Üçüncü baskıdan çeviri. Editör: Prof. Dr. İsmail Türkan. Palme Yayıncılık. Ankara, s. 591-602.
19. Velikova, V., Yordanov, I., Edrava, A., 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science. 151, 59-66.
20. Wang, S.Y., Jiao, J.H., and Faust, M., 1991. Changes in ascorbate, glutathione and related enzyme activities during thidiazuron-induced bud break of apple. Plant Physiol. 82 (2), 231-236.