Derleme
BibTex RIS Kaynak Göster

Plastik Biyobozunması: Mikrobiyal Enzimlerin Potansiyeli

Yıl 2025, Cilt: 1 Sayı: 2 , 57 - 78 , 29.07.2025
https://izlik.org/JA85EJ46UY

Öz

Plastik bazlı kirlilik, hem çevre hem de insan sağlığı açısından ciddi bir tehdit oluşturmaktadır. Karasal ve sucul ekosistemler üzerindeki olumsuz etkileri nedeniyle, atık plastiklerin bertarafı için sürdürülebilir ve çevre dostu yöntemlerin geliştirilmesi büyük önem taşımaktadır. Geleneksel bertaraf yöntemleri olan yakma ve çöplükte depolama, zararlı yan ürünlerin oluşumuna neden olarak çevreye ek zararlar verebilmektedir. Son yıllarda yapılan çalışmalar, bazı plastik türlerinin enzimatik aktiviteye sahip mikroorganizmalar tarafından biyolojik olarak parçalanabildiğini ortaya koymuştur. Biyolojik bozunma, plastik atıkların parçalanmasında mikroorganizmalardan izole edilen enzimlerin kullanıldığı etkili bir yöntem olarak öne çıkmaktadır. Bu enzimler, plastik yüzeylerle etkileşime girerek yüksek moleküler ağırlıklı polimer zincirlerini daha küçük birimlere hidrolize eder. Oluşan ara ürünler mikroorganizmalar tarafından karbon kaynağı olarak kullanılabilir ve nihayetinde karbondioksit ile suya dönüştürülür. Enzimler tarafından gerçekleştirilen oksidasyon veya hidroliz tepkimeleri, polimerlerin hidrofilik özelliklerini artıran fonksiyonel gruplar oluşturarak parçalanmayı kolaylaştırır. Bu sayede bazı plastiklerin bozunması birkaç gün içinde mümkün hale gelmektedir. Bu derlemede, mikrobiyal kaynaklı enzimler ve bu enzimlerin plastik polimerlerin biyobozunurluğunda kullanımı değerlendirilmiştir.

Etik Beyan

Bu çalışmada insan ya da hayvan denek kullanılmamıştır. Etik kurul onayı gerekmemektedir.

Kaynakça

  • 1. Wright SL, Kelly FJ. Plastic and human health: a micro issue? Environ Sci Technol. 2017;51(12):6634–6647. https://doi.org/10.1021/acs.est.7b00423
  • 2. Gall SC, Thompson RC. The impact of debris on marine life. Mar Pollut Bull. 2015;92(12):170–179. https://doi.org/10.1016/j.marpolbul.2014.12.041
  • 3. Rochman CM, Hoh E, Kurobe T, Teh SJ. Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress. Sci Rep. 2013;3:3263. https://doi.org/10.1038/srep03263
  • 4. Waring RH, Harris RM, Mitchell SC. Plastic contamination of the food chain: A threat to human health? Maturitas. 2018;115:64–68. https://doi.org/10.1016/j.maturitas.2018.06.010
  • 5. Rillig MC. Microplastic in terrestrial ecosystems and the soil? Environ Sci Technol. 2012;46(12):6453–6454. https://doi.org/10.1021/es302011r
  • 6. Food and Agriculture Organization. Assessment of agricultural plastics and their sustainability: A call for action. Rome: FAO; 2021. https://doi.org/10.4060/cb7856en
  • 7. PlasticsEurope. Plastics – the Fast Facts. 2024. Available from: https://plasticseurope.org/knowledge-hub/plastics-the-fast-facts-2024/ 8. PlasticsEurope. The Circular Economy for Plastics – A European Analysis. 2024. Available from: https://plasticseurope.org/knowledge-hub/the-circular-economy-for-plastics-a-european-analysis-2024/ 9. WWF Türkiye. Plastik Atıkların Yönetimi: Türkiye Raporu. 2019. Available from: https://www.wwf.org.tr/
  • 10. Hahladakis JN, Velis CA, Weber R, Iacovidou E, Purnell P. An overview of chemical additives present in plastics: Migration, release, fate and environmental impact during their use, disposal and recycling. J Hazard Mater. 2018;344:179-199. https://doi.org/10.1016/j.jhazmat.2017.10.014
  • 11. Ellen MacArthur Foundation. Towards the Circular Economy: Economic and Business Rationale for an Accelerated Transition. 2013. Cowes, UK: Ellen MacArthur Foundation.
  • 12. WRAP. A Roadmap to 2025 - The UK Plastics Pact. 2018. Available from: https://www.wrap.org.uk/content/the-uk-plastics-pact-roadmap-2025
  • 13. Defra UK. Guidance on applying the Waste Hierarchy. 2011. Available from : https://www.gov.uk/government/publications/guidance-on-applying-the-waste-hierarchy
  • 14. Ellen MacArthur Foundation. The Global Commitment 2024 Progress Report. 2014. Available from: https://www.ellenmacarthurfoundation.org/global-commitment-2024/overview
  • 15. García-Depraect O, Bordel S, Lebrero R, Santos-Beneit F, Börner RA, Börner T, Muñoz R. Inspired by nature: Microbial production, degradation and valorization of biodegradable bioplastics for life-cycle-engineered products. Biotechnol Adv. 2021;53:107772. https://doi.org/10.1016/j.biotechadv.2021.107772
  • 16. Highmoore JF, Kariyawasam LS, Trenor SR, Yang Y. Design of depolymerizable polymers toward a circular economy. Green Chem. 2024;26:(5):2384–2420. https://doi.org/10.1039/D3GC04215D
  • 17. Carbios. Carbios: A Circular Economy for Plastics. Carbios. 2024. Available from: https://www.carbios.com/en/
  • 18. Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, et al. A bacterium that degrades and assimilates poly(ethylene terephthalate). Science. 2016;351(6278):1196–1199. https://doi.org/10.1126/science.aad6359 19. Danso D, Chow J, Streit WR. Plastics: Environmental and biotechnological perspectives on microbial degradation. Appl Environ Microbiol. 2019;85(19):e01095–19. https://doi.org/10.1128/AEM.01095-19 20. Wei R, Zimmermann W. Microbial enzymes for the recycling of recalcitrant petroleum‐based plastics: How far are we? Microb Biotechnol. 2017;10(6):1308–1322. https://doi.org/10.1111/17517915.12710 21. Vona IA, Costanza JR, Cantor HA, Robert WJ. Manufacture of plastics. Wiley, New York. 1965:1(66):141–142.
  • 22. Shah AA, Hasan F, Hameed A, Ahmed S. Biological degradation of plastics:A comprehensive review. Biotechnol Adv. 2008;26:246–265. https://doi:10.1016/j.biotechadv.2007.12.005
  • 23. Tokiwa Y, Calabia BP, Ugwu CU, Aiba S. Biodegradability of Plastics. Int J Mol Sci. 2009;10:3722–3742. https://doi.org/10.3390/ijms10093722
  • 24. Gross RA, Kalra B. Biodegradable polymers for the environment. Science. 2002;297(5582):803–807. https://doi.org/10.1126/science.297.5582.803
  • 25. Williams DF. Enzymic hydrolysis of polylactic acid. Eng Med. 1981;10(1):5–7. https://doi.org/10.1243/EMED_JOUR_1981_010_004
  • 26. Montazer Z, Habibi Najafi MB, Levin DB. Challenges with verifying microbial degradation of polyethylene. Polymers. 2020;12:123. https://doi.org/10.3390/polym12010123
  • 27. Mierzwa-Hersztek M., Gondek K., Kopeć M. Degradation of polyethylene and biocomponent-derived polymer materials: An overview. J Polym Environ. 2019;27:600–611. https://doi.org/10.1007/s10924-019-01368-4
  • 28. Kaushal J, Khatri M, Arya S. Recent insight into enzymatic degradation of plastics prevalent in the environment: A mini - review. Clean Eng Technol. 2021;2:100083. https://doi.org/10.1016/j.clet.2021.100083
  • 29. Sang T, Wallis CJ, Hill G, Britovsek GJ. Polyethylene terephthalate degradation under natural and accelerated weathering conditions. Eur Polym J. 2020;136:109873. https://doi.org/10.1016/j.eurpolymj.2020.109873
  • 30. Raheem AB, Noor ZZ, Hassan A, Abd Hamid MK, Samsudin SA, Sabeen AH. Current developments in chemical recycling of post-consumer polyethylene terephthalate wastes for new materials production: A review. J Clean Prod. 2019;225:1052–1064. https://doi.org10.1016/j.jclepro.2019.04.019
  • 31. Zimmermann W, Billig S. Enzymes for the biofunctionalization of poly(ethylene terephthalate). Adv Biochem Eng Biotechnol. 2011;125:97–120. https://doi.org/10.1007/10_2010_87
  • 32. Fischer-Colbrie G, Heumann S, Liebminger S, Almansa E, Cavaco-Paulo A, Guebitz GM. New enzymes with potential for PET surface modification. Biocatal Biotransform. 2004;22:341–346. https://doi.org/10.1080/10242420400024565
  • 33. Chandramouli Swamy TM, Nagarathna SV, Reddy PV, Nayak AS. Efficient biodegradation of Polyethylene terephthalate (PET) plastic by Gordonia sp. CN2K isolated from plastic contaminated environment. Ecotoxicol Environ Saf. 2024;281:116635. https://doi.org/10.1016/j.ecoenv.2024.116635
  • 34. Torena P, Alvarez-Cuenca M, Reza M. Biodegradation of polyethylene terephthalate microplastics by bacterial communities from activated sludge. Can J Chem Eng. 2021;99(S1):S69–S82. https://doi.org/10.1002/cjce.24015
  • 35. Yip A, McArthur OD, Ho KC, Aucoin MG, Ingalls BP. Degradation of polyethylene terephthalate (PET) plastics by wastewater bacteria engineered via conjugation. Microb Biotechnol. 2024;17:e70015. https://doi.org/10.1111/1751-7915.70015
  • 36. Roman EKB, Ramos MA, Tomazetto G, Foltran BB, Galvão MH, Ciancaglini I, et al. Plastic-degrading microbial communities reveal novel microorganisms, pathways, and biocatalysts for polymer degradation and bioplastic production. Sci Total Environ. 2024;949:174876. https://doi.org/10.1016/j.scitotenv.2024.174876 37. Carniel A, Valoni É, Junior JN, Gomes A, Castro AM. Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid. Process Biochem. 2017;59:84–90. https://doi.org/10.1016/j.procbio.2016.07.023
  • 38. Rowe L, Howard GT. Growth of Bacillus subtilis on polyurethane and the purification and characterization of a polyurethanase-lipase enzyme. Int Biodeterior Biodegrad. 2002;50:33–40. https://doi.org/10.1016/S0964-8305(02)00047-1
  • 39. Nakkabi A, Sadiki M, Fahim M, Ittobane N, Ibnsouda Koraichi S, Barkai H, et al. Biodegradation of poly(ester urethane)s by Bacillus subtilis. Int J Environ Res. 2015;9(1):157–162
  • 40. Razak NASA, Habib S, Shukor MYA, Alias SA, Smykla J, Yasid NA. Isolation and Characterisation of Polypropylene Microplastic-Utilising Bacterium from the Antarctic Soil. arXiv. 2024. https://doi.org/10.48550/arXiv.2401.02096
  • 41. Peng BY, Chen Z, Chen J, Yu H, Zhou X, Criddle CS, et al. Biodegradation of polyvinyl chloride (PVC) in Tenebrio molitor (Coleoptera: Tenebrionidae) larvae. Environ Int. 2020;145:106106. https://doi.org/10.1016/j.envint.2020.106106
  • 42. Sadat-Shojai M, Bakhshandeh GR. Recycling of PVC wastes. Polym Degrad Stab. 2011;96:404–415. https://doi.org/10.1016/j.polymdegradstab.2010.12.001
  • 43. Winkler DE. Mechanism of polyvinyl chloride degradation and stabilization. J Polym Sci. 1959;35:3–16. https://doi.org/10.1002/pol.1959.1203512802
  • 44. Pospíšil J, Horák Z, Kruliš Z, Nešpůrek S, Kuroda SI. Degradation and aging of polymer blends I. Thermomechanical and thermal degradation. Polym Degrad Stab. 1999;65:405–414. https://doi.org/10.1016/S0141-3910(99)00029-4
  • 45. Zhang Z, Peng H, Yang D, Zhang G, Zhang J, Ju F. Polyvinyl chloride degradation by a bacterium isolated from the gut of insect larvae. Nat Commun. 2022;13(1):5360. doi:10.1038/s41467-022-32903-y
  • 46. Mohanan N, Montazer Z, Sharma PK, Levin DB. Microbial and enzymatic degradation of synthetic plastics. Front Microbiol. 2020;11:2837. https://doi.org/10.3389/fmicb.2020.580709
  • 47. Chaudhary AK, Vijayakumar RP. Studies on biological degradation of polystyrene by pure fungal cultures. Environ Dev Sustain. 2020;22:4495–4508. https://doi.org/10.1007/s10668-019-00394-5
  • 48. Ho BT, Roberts TK, Lucas S. An overview on biodegradation of polystyrene and modified polystyrene: The microbial approach. Crit Rev Biotechnol. 2017;38:308–320. https://doi.org/10.1080/07388551.2017.1355293
  • 49. Krueger MC, Seiwert B, Prager A, Zhang S, Abel B, Harms H, Schlosser D. Degradation of polystyrene and selected analogues by biological Fenton chemistry approaches: Opportunities and limitations. Chemosphere. 2017;173:520–528 https://doi.org/10.1016/j.chemosphere.2017.01.089
  • 50. Kim HR, Koh HY, Shin H, Suh DE, Lee S, Choi D. Enhancing the oxidation of polystyrene through a homogeneous liquid degradation system for effective microbial degradation. Front Microbiol. 2024;15:1509603. https://doi.org/10.3389/fmicb.2024.1509603
  • 51. Wang J, Liu R, Zhao S, Zhang B, Shao Z. Construction of an efficient polystyrene-degrading microbial consortium based on degrading and non-degrading bacteria predominant in biofilms of marine plastic debris. Front Mar Sci. 2025;12. https://doi.org/10.3389/fmars.2025.1569583
  • 52. Itävaara M, Karjomaa S, Selin JF. Biodegradation of polylactide in aerobic and anaerobic thermophilic conditions. Chemosphere. 2002;46:879–885. https://doi.org/10.1016/S0045-6535(01)00163-1
  • 53. Cui L, Wang X, Szarka G, Hegyesi N, Wang Y, Sui X, et al. Quantitative analysis of factors determining the enzymatic degradation of poly(lactic acid). Int J Biol Macromol. 2022;209:1703–1709. https://doi.org/10.1016/j.ijbiomac.2022.04.121
  • 54. Shin N, Kim SH, Oh J, Kim S, Lee Y, Shin Y, Choi S, Bhatia SK, Kim Y-G, Yang Y-H. Reproducible Polybutylene Succinate (PBS)-Degrading Artificial Consortia by Introducing the Least Type of PBS-Degrading Strains. Polymers. 2024;16(5):651. https://doi.org/10.3390/polym16050651 55. Howard GT. Biodegradation of polyurethane: A review. Int Biodeterior Biodegrad. 2002;49:245–252. https://doi.org/10.1016/S0964-8305(02)00051-3
  • 56. Álvarez-Barragán J, Domínguez-Malfavón L, Vargas-Suárez M, González-Hernández R, Aguilar-Osorio G, Loza-Tavera H. Biodegradative activities of selected environmental fungi on a polyester polyurethane varnish and polyether polyurethane foams. Appl Environ Microbiol. 2016;82:5225–5235. https://doi.org/10.1128/AEM.01344-16
  • 57. Nakajima-Kambe T, Shigeno-Akutsu Y, Nomura N, Onuma F, Nakahara T. Microbial degradation of polyurethane, polyester polyurethanes and polyether polyurethanes. Appl Microbiol Biotechnol. 1999;51:134–140. https://doi.org/10.1007/s002530051373
  • 58. Ru J, Chen X, Dong X, Hu L, Zhang J, Yang Y. Discovery of a polyurethane-degrading enzyme from the gut bacterium of plastic-eating mealworms. J Hazard Mater. 2024;480:136159. https://doi.org/10.1016/j.jhazmat.2024.136159
  • 59. Al Hosni AS, Pittman JK, Robson GD. Microbial degradation of four biodegradable polymers in soil and compost demonstrating polycaprolactone as an ideal compostable plastic. Waste Manag. 2019;97:105–114. https://doi.org/10.1016/j.wasman.2019.07.042
  • 60. Geyer R, Jambeck JR, Law KL. Production, use, and fate of all plastics ever made. Sci Adv. 2017;3:1700782. https://doi.org/10.1126/sciadv.1700782
  • 61. Badino SF, Bååth JA, Borch K, Jensen K, Westh P. Adsorption of enzymes with hydrolytic activity on polyethylene terephthalate. Enzyme Microb Technol. 2021;152:109937. https://doi.org/10.1016/j.enzmictec.2021.109937
  • 62. Taghavi N, Zhuang WQ, Baroutian S. Enhanced biodegradation of non-biodegradable plastics by UV radiation: Part 1. J Environ Chem Eng. 2021;9:106464. https://doi.org/10.1016/j.jece.2021.106464
  • 63. Vedrtnam A, Kumar S, Chaturvedi S. Experimental study on mechanical behavior, biodegradability, and resistance to natural weathering and ultraviolet radiation of wood-plastic composites. Compos B Eng. 2019;176:107282. https://doi.org/10.1016/j.compositesb.2019.107282
  • 64. Arutchelvi J, Sudhakar M, Arkatkar A, Doble M, Bhaduri S, Uppara PV. Biodegradation of polyethylene and polypropylene. IJBT. 2008;7(1):9–22
  • 65. Amobonye A, Bhagwat P, Singh S, Pillai S. Plastic biodegradation: Frontline microbes and their enzymes. Sci Total Environ. 2021;759:143536. https://doi.org/10.1016/j.scitotenv.2020.143536
  • 66. Yuan J, Ma J, Sun Y, Zhou T, Zhao Y, Yu F. Microbial degradation and other environmental aspects of microplastics/plastics. Sci Total Environ. 2020;715:136968. https://doi.org/10.1016/j.scitotenv.2020.136968
  • 67. Wang GX, Huang D, Ji JH, Völker C, Wurm FR. Seawater-Degradable Polymers-Fighting the Marine Plastic Pollution. Adv Sci. 2020;8:2001121. https://doi.org/10.1002/advs.202001121
  • 68. Emadian SM, Onay TT, Demirel B. Biodegradation of bioplastics in natural environments. Sci Total Environ. 2017;574:1079-92. https://doi.org/10.1016/j.scitotenv.2016.09.162
  • 69. Chigwada AD, Tekere M. The plastic and microplastic waste menace and bacterial biodegradation for sustainable environmental clean-up: a review. Environ Res. 2023;10:116110. https://doi.org/10.1016/j.envres.2023.116110
  • 70. Sarsan S, Kodaparthi A, Susmitha B. Microbial enzymes in plastic degradation. In Developments in Applied Microbiology and Biotechnology. Microbial Essentialism. 2024; 207–242. https://doi.org/10.1016/b978-0-443-13932-1.00005-2
  • 71. Temporiti MEE, Nicola L, Nielsen E, Tosi S. Fungal Enzymes Involved in Plastics Biodegradation. Microorganisms. 2022;10(6):1180. Published 2022 Jun 8. https://doi.org/10.3390/microorganisms10061180
  • 72. Glaser JA. Biological degradation of polymers in the environment. In: Kästner M, Trapp S, editors. Plastics in the Environment. London: IntechOpen; 2019. p.13–30. https://doi.org/10.5772/intechopen.85124
  • 73. Vaishnav A, Lal J, Singh N, Pati BK, Mehta NK, Priyadarshini MB. Role of microbial enzymes and their modification for plastic biodegradation. In: Advanced Strategies for Biodegradation of Plastic Polymers. Springer. 2024;373–403. https://doi.org/10.1007/978-3-031-55661-6_16
  • 74. Asiandu AP, Wahyudi A, Sari SW. A review: plastics waste biodegradation using plastics-degrading bacteria. J Environ Treat Tech. 2021;9(1):148–157. https://doi.org/10.47277/JETT/9(1)157
  • 75. Srikanth M, Sandeep TSRS, Sucharitha K, Godi S. Biodegradation of plastic polymers by fungi: a brief review. Bioresour Bioprocess. 2022;9(1):42. https://doi.org/10.1186/s40643-022-00532-4
  • 76. Albertsson AC, Karlsson S. Aspects of biodeterioration of inert and degradable polymers. Int Biodeterior Biodegrad. 1993;31:161–170. https://doi.org/10.1016/0964-8305(93)90002-J
  • 77. Ammala A, Bateman S, Deana K, Petinakis E, Sangwan P, Wong S, et al. An overview of degradable and biodegradable polyolefins. Prog Polym Sci. 2011;36:1015–1049. https://doi.org/10.1016/j.progpolymsci.2010.12.002
  • 78. Fesseha H, Abebe F. Degradation of plastic materials using microorganisms: A review. Public Health Open J. 2019;4(2):57–63. https://doi.org/10.17140/PHOJ-4-136
  • 79. Urbanek AK, Mirończuk AM, García-Martín A, Saborido A, de la Mata I, Arroyo M. Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester type plastics. Biochim Biophys Acta Proteins Proteom. 2020;1868(2):140315. https://doi.org/10.1016/j.bbapap.2019.140315
  • 80. Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, et al. An engineered PET depolymerase to break down and recycle plastic bottles. Nature. 2020;580(7802):216–219. https://doi.org/10.1038/s415860202149-4
  • 81. Kale G, Kijchavengkul T, Auras R, Rubino M, Selke SE, Singh SP. Compostability of bioplastic packaging materials: an overview. Macromol Biosci. 2007;7(3):255–277. https://doi.org/10.1002/mabi.200600168
  • 82. Narancic T, Verstichel S, Reddy Chaganti S, et al. Biodegradable plastic blends create new possibilities for end of life management of plastics but they are not a panacea for plastic pollution. Environ Sci Technol. 2018;52(18):10441–10452. https://doi.org/10.1021/acs.est.8b02963
  • 83. Egmond MR, de Vlieg J. Fusarium solani pisi cutinase. Biochimie. 2000;82(11):1015–1021. https://doi.org/10.1016/s0300-9084(00)01183-4
  • 84. Chen S, Su L, Chen J, Wu J. Cutinase: characteristics, preparation and application. Biotechnol Adv. 2013;31:1754–1767. https://doi.org/10.1016/j.biotechadv.2013.09.005
  • 85. Sharma AK, Tiwari RP, Hoondal GS. Properties of a thermostable and solvent stable extracellular lipase from a Pseudomonas sp. AG-8. J Basic Microbiol. 2001;41(6):363–366. https://doi.org/10.1002/1521-4028(200112)41:6<363::AID-JOBM363>3.0.CO;2-C
  • 86. Houde A, Kademi A, Leblanc D. Lipases and their industrial applications: An overview. Appl Biochem Biotechnol. 2004;118(1–3):155–170. https://doi.org/10.1385/abab:118:1-3:155
  • 87. Purdy RE, Kolattukudy PE. Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi. Biochemistry. 1975;14(13):2832–2840. https://doi.org/10.1021/bi00684a007
  • 88. Dutta K, Sen S, Veeranki VD. Production, characterization and applications of microbial cutinases. Process Biochem. 2009;44:127–134. https://doi.org/10.1016/j.procbio.2008.09.008
  • 89. Alisch M, Feuerhack A, Müller H, Mensak B, Andreaus J, Zimmermann W. Biocatalytic modification of polyethylene terephthalate fibres by esterases from actinomycete isolates. Biocatal Biotransform. 2004;22(5–6):347–351. https://doi.org/10.1080/10242420400025877
  • 90. Wang G, Guo Z, Zhang X, Wu H, Bai XM, Zhang H, et al. Heterologous expression of pediocin/papA in Bacillus subtilis. Microb Cell Fact. 2022;21:104. https://doi.org/10.1186/s12934-022-01829-x
  • 91. Maurya A, Bhattacharya A, Khare SK. Enzymatic remediation of polyethylene terephthalate (PET)-based polymers for effective management of plastic wastes: An overview. Front Bioeng Biotechnol. 2020;8:602325. https://doi.org/10.3389/fbioe.2020.602325
  • 92. Baker PJ, Poultney C, Liu Z, Gross R, Montclare JK. Identification and comparison of cutinases for synthetic polyester degradation. Appl Microbiol Biotechnol. 2012;93(1):229–240. https://doi.org/10.1007/s00253-011-3402-4
  • 93. Hellesnes KN, Vijayaraj S, Fojan P, Petersen E, Courtade G. Biochemical characterization and NMR study of a PET-Hydrolyzing cutinase from Fusarium solani pisi. Biochemistry. 2023;62(8):1369–1375. https://doi.org/10.1021/acs.biochem.2c00619
  • 94. de Castro AM, Carniel A, Nicomedes Junior J, da Conceição Gomes A, Valoni É. Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources. J Ind Microbiol Biotechnol. 2017;44(6):835–844. https://doi.org/10.1007/s10295-017-1942-z
  • 95. Dimarogona M, Nikolaivits E, Kanelli M, Christakopoulos P, Sandgren M, Topakas E. Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential. Biochim Biophys Acta. 2015;1850(11):2308–2317. https://doi.org/10.1016/j.bbagen.2015.08.009
  • 96. Ronkvist ÅM, Xie W, Lu W, Gross RA. Cutinase-catalyzed hydrolysis of poly (ethylene terephthalate). Macromolecules. 2009;42:5128–5138. https://doi.org/10.1021/ma9005318
  • 97. Murphy CA, Cameron JA, Huang SJ, Vinopal RT. Fusarium polycaprolactone depolymerase is cutinase. Appl Environ Microbiol. 1996;62(2):456–460. https://doi.org/10.1128/aem.62.2.456–460.1996
  • 98. Adıgüzel AO, Tunçer M. Purification and characterization of cutinase from Bacillus sp. KY0701 isolated from plastic wastes. Prep Biochem Biotechnol. 2017;47:925–933. https://doi.org/10.1080/10826068.2017.1365245 99. Ho BT, Roberts TK, Lucas S. An overview on biodegradation of polystyrene and modified polystyrene: the microbial approach. Crit Rev Biotechnol. 2018;38:308–320. https://doi.org/10.1080/07388551.2017.1355293
  • 100. Hu X, Gao Z, Wang Z, Su T, Yang L, Li P. Enzymatic degradation of poly (butylene succinate) by cutinase cloned from Fusarium solani. Polym Degrad Stab. 2016;134:211–219. https://doi.org/10.1016/j.polymdegradstab.2016.10.012
  • 101. Van Gemeren IA, Beijersbergen A, van den Hondel CA, Verrips CT. Expression and secretion of defined cutinase variants by Aspergillus awamori. Appl Environ Microbiol. 1998;64(8):2794–2799. https://doi.org/10.1128/AEM.64.8.2794-2799.1998
  • 102. Phytian SJ. Esterases. In: Kelly DR, editor. Biotechnology. 2nd ed. Weinheim: Wiley-VCH; 1998:193–241.
  • 103. Zhang L, Cao K, Liu H, Wang Y, Zhang B, Han H, et al. Discovery of a polyester polyurethane-degrading bacterium from a coastal mudflat and identification of its degrading enzyme. J Hazard Mater. 2025;483:136659. https://doi.org/10.1016/j.jhazmat.2024.136659
  • 104. Lin J, Sun K, Ma L, Li C, Tong H, Wang Z. Enzymatic degradation of polybutylene succinate by recombinant cutinase cloned from Paraphoma chrysanthemicola. J Environ Manage. 2025;375:124288. https://doi.org/10.1016/j.jenvman.2025.124288
  • 105. Bornscheuer UT, Kazlauskas RJ. Hydrolases in organic synthesis: regio- and stereoselective biotransformations. 1999. Wiley-VCH, Weinheim.
  • 106. Hoshino A, Isono Y. Degradation of aliphatic polyester films by commercially available lipases with special reference to rapid and complete degradation of poly(L-lactide) film by lipase PL derived from Alcaligenes sp. Biodegradation. 2002;13(2):141–147. https://doi.org/10.1023/a:1020450326301
  • 107. Safdar A, Ismail F, Imran M. Biodegradation of synthetic plastics by the extracellular lipase of Aspergillus niger. Environ Adv. 2024;17:100563. https://doi.org/10.1016/j.envadv.2024.100563.
  • 108. Kawai F, Kawabata T, Oda M. Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields. Appl Microbiol Biotechnol. 2019;103(11):4253–4268. https://doi.org/10.1007/s00253-019-09717-y
  • 109. Khairul Anuar NFS, Huyop F, Ur-Rehman G, Abdullah F, Normi YM, Sabullah MK, et al. An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation. Int J Mol Sci. 2022;23(20):12644. https://doi.org/10.3390/ijms232012644
  • 110. Vidal P, Martínez-Martínez M, Fernandez-Lopez L, Roda S, Méndez-García C, Golyshina OV, et al. Metagenomic mining for esterases in the microbial community of los rueldos acid mine drainage formation. Front Microbiol. 2022;13:868839. https://doi.org/10.3389/fmicb.2022.868839
  • 111. Khan S, Nadir S, Shah ZU, Shah AA, Karunarathna SC, Xu J, et al. Biodegradation of polyester polyurethane by Aspergillus tubingensis. Environ Pollut. 2017;225:469–480. https://doi.org/10.1016/j.envpol.2017.03.012
  • 112. Brunner I, Fischer M, Rüthi J, Stierli B, Frey B. Ability of fungi isolated from plastic debris floating in the shoreline of a lake to degrade plastics. PLoS One. 2018;13(8):e0202047. https://doi.org/10.1371/journal.pone.0202047
  • 113. Barclay A, Acharya KR. Engineering plastic eating enzymes using structural biology. Biomolecules. 2023;13(9):1407. https://doi.org/10.3390/biom13091407
  • 114. Chen CC, Han X, Ko TP, Liu W, Guo RT. Structural studies reveal the molecular mechanism of PETase. FEBS J. 2018;285(20):3717-3723. https://doi.org/10.1111/febs.14612
  • 115. Kawai F, Kawabata T, Oda M. Current state and perspectives related to the polyethylene terephthalate hydrolases available for biorecycling. ACS Sustain Chem Eng. 2020;8:8894–8908. https://doi.org/10.1021/acssuschemeng.0c01638
  • 116. Palm GJ, Reisky L, Böttcher D, Müller H, Michels EAP, Walczak MC, et al. Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate. Nat Commun. 2019;10(1):1717. https://doi.org/10.1038/s41467-019-09326-3
  • 117. Han Z, Nina MRH, Zhang X, Huang H, Fan D, Bai Y. Discovery and characterization of two novel polyethylene terephthalate hydrolases: one from a bacterium identified in human feces and one from the Streptomyces genus. J Hazard Mater. 2024;472:134532. https://doi.org/10.1016/j.jhazmat.2024.134532
  • 118. Mohanan N, Montazer Z, Sharma PK, Levin DB. Microbial and enzymatic degradation of synthetic plastics. Front Microbiol. 2020;11:580709. https://doi.org/10.3389/fmicb.2020.580709
  • 119. Furukawa M, Kawakami N, Oda K, Miyamoto K. Acceleration of Enzymatic Degradation of Poly(ethylene terephthalate) by Surface Coating with Anionic Surfactants. ChemSusChem. 2018;11(23):4018-4025. https://doi.org/10.1002/cssc.201802096
  • 120. Ikehata K, Buchanan ID, Smith DW. Recent developments in the production of extracellular fungal peroxidases and laccases for waste treatment. J Environ Eng Sci. 2004;3:1–19. https://doi.org/10.1139/s03-077 121. Hofrichter M, Ullrich R. Heme-thiolate haloperoxidases: versatile biocatalysts with biotechnological and environmental significance. Appl Microbiol Biotechnol. 2006;71(3):276–288. https://doi.org/10.1007/s00253-006-0417-3
  • 122. Conesa A, Punt PJ, van den Hondel CA. Fungal peroxidases: Molecular aspects and applications. J Biotechnol. 2002;93:143–158. https://doi.org/10.1016/S0168-1656(01)00394-7
  • 123. Maciel MJM, Ribeiro HCT. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Electron J Biotechnol. 2010;13:14–15. https://doi.org/10.2225/vol13-issue6-fulltext-2
  • 124. Iiyoshi Y, Tsutsumi Y, Nishida T. Polyethylene degradation by lignin-degrading fungi and manganese peroxidase. J Wood Sci. 1998;44:222–229. https://doi.org/10.1007/BF00521967
  • 125. Ganesh P, Dineshraj D, Yoganathan K. Production and screening of depolymerising enzymes by potential bacteria and fungi isolated from plastic waste dump yard sites. Int J Appl Res. 2017;3(3):693–695
  • 126. Olivieri G, Wijffels RH, Marzocchella A, Russo ME. Bioreactor and bioprocess design issues in enzymatic hydrolysis of lignocellulosic biomass. Catalysts. 2021;11:680. https://doi.org/10.3390/catal11060680
  • 127. Muangchinda C, Pinyakong O. Enrichment of LDPE-degrading bacterial consortia: Community succession and enhanced degradation efficiency through various pretreatment methods. Sci Rep. 2024;14(1):28795. https://doi.org/10.1038/s41598-024-80306-4
  • 128. Cazaudehore G, Guyoneaud R, Vasmara C, Greuet P, Gastaldi E, Marchetti R, et al. Impact of mechanical and thermo-chemical pretreatments to enhance anaerobic digestion of poly(lactic acid). Chemosphere. 2022;297:133986. https://doi.org/10.1016/j.chemosphere.2022.133986
  • 129. Arkatkar A, Arutchelvi J, Bhaduri S, Uppara PV, Doble M. Degradation of unpretreated and thermally pretreated polypropylene by soil consortia. Int Biodeterior Biodegrad. 2009;63(1):106–111. https://doi.org/10.1016/j.ibiod.2008.06.005
  • 130. Edge M, Hayes M, Mohammadian M, Allen NS, Jewitt TS, Brems K, et al. Aspects of poly(ethylene terephthalate) degradation for archival life and environmental degradation. Polym Degrad Stab. 1991;32(2):131–153. https://doi.org/10.1016/0141-3910(91)90047-U
  • 131. Rostampour S, Cook R, Jhang SS, Li Y, Fan C, Sung LP. Changes in the Chemical Composition of Polyethylene Terephthalate under UV Radiation in Various Environmental Conditions. Polymers. 2024;16(16):2249. https://doi.org/10.3390/polym16162249
  • 132. Jiang Z, Chen X, Xue H, Li Z, Lei J, Yu M, et al. Novel polyurethane-degrading cutinase BaCut1 from Blastobotrys sp. G-9 with potential role in plastic bio-recycling. J Hazard Mater. 2024;472:134493. https://doi.org/10.1016/j.jhazmat.2024.134493
  • 133. Li S, Zhang, W. Computational identification of plastic-degrading enzymes in ocean microbiomes. Sci Rep. 2025;15:15332. https://doi.org/10.1038/s41598-025-99275-3
  • 134. Zhang Y, Wang Y, Wang B, Xia X, Wang T, Lu Y. Mild PET degradation by enzymes coupled with magnetic and optical manipulation. J Hazard Mater. 2025;494:138663. https://doi.org/10.1016/j.jhazmat.2025.138663 135. Shi K, Su T, Wang Z. Comparison of poly(butylene succinate) biodegradation by Fusarium solani cutinase and Candida antarctica lipase. Polym Degrad Stab. 2019;164:55–60. https://doi.org/10.1016/j.polymdegradstab.2019.04.005
  • 136. Herrero Acero E, Ribitsch D, Dellacher A, Zitzenbacher S, Marold A, Steinkellner G, et al. Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis. Biotechnol Bioeng. 2013;110(10):2581–2590. https://doi.org/10.1002/bit.24930 137. Ribitsch D, Herrero Acero E, Greimel KJ, Eiteljörg I, Trotscha E, Freddi G, et al. Characterization of a new cutinase from Thermobifida alba for PET-surface hydrolysis. Biocatal Biotransform. 2012;30(1):2–9. https://doi.org/10.3109/10242422.2012.644435
  • 138. Din SU, Kalsoom, Satti SM, Uddin S, Mankar SV, Ceylan E, et al. The purification and characterization of a cutinase-like enzyme with activity on polyethylene terephthalate (pet) from a newly ısolated bacterium Stenotrophomonas maltophilia PRS8 at a mesophilic temperature. Appl Sci. 2023;13(6):3686. https://doi.org/10.3390/app13063686
  • 139. Jung HW, Mei-Kwei Y, Su RC. Purification, characterization, and gene cloning of an Aspergillus fumigatus polyhydroxybutyrate depolymerase used for degradation of polyhydroxybutyrate, polyethylene succinate, and polybutylene succinate. Polym Degrad Stab. 2018;154:186–194. https://doi.org/10.1016/j.polymdegradstab.2018.06.002
  • 140. Gautam R, Bassi A, Yanful E. Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium. Biotechnology Letters. 2007;29:1081–6. https://doi.org/10.1007/s10529-007-9354-1
  • 141. Nechwatal A, Blokesch A, Nicolai M, Krieg M, Kolbe A, Wolf M, et al. A contribution to the investigation of enzyme catalysed hydrolysis of poly(ethylene terephthalate) oligomers. Macromol Mater Eng. 2006;291:1486–1494. https://doi.org/10.1002/mame.200600204 142. Akutsu Y, Nakajima-Kambe T, Nomura N, Nakahara T. Purification and Properties of a Polyester Polyurethane-Degrading Enzyme from Comamonas acidovorans TB-35. Appl Environ Microbiol. 1998;64(1):62–67. https://doi.org/10.1128/AEM.64.1.62-67.1998

Plastic Biodegradation: Potential of Microbial Enzymes

Yıl 2025, Cilt: 1 Sayı: 2 , 57 - 78 , 29.07.2025
https://izlik.org/JA85EJ46UY

Öz

Plastic-based pollution poses a grave threat to environmental and human health. Plastic-based pollution poses a serious threat to both environmental and human health. Due to its adverse effects on terrestrial and aquatic ecosystems, developing sustainable and eco-friendly methods for plastic waste management has become essential. Traditional disposal methods such as incineration and landfilling are problematic, as they often generate hazardous byproducts and contribute to further environmental degradation. Recent studies have demonstrated that certain types of plastics can be biologically degraded by microorganisms possessing enzymatic activity. Biodegradation, which utilizes enzymes isolated from microorganisms to break down plastic waste, has emerged as a promising and environmentally friendly alternative. These enzymes interact with plastic surfaces and hydrolyze high molecular weight polymer chains into smaller units. The resulting byproducts can then be utilized by microbes as carbon sources and ultimately converted into carbon dioxide and water. Enzymatic oxidation or hydrolysis introduces functional groups that enhance the hydrophilicity of polymers, facilitating the breakdown of high molecular weight plastics into lower molecular weight fragments. This process enables the degradation of some plastics within a matter of days. In this review, microbial-derived enzymes and their applications in the biodegradability of plastic polymers are evaluated.

Etik Beyan

This study did not involve human or animal subjects. Therefore, ethical approval was not required.

Kaynakça

  • 1. Wright SL, Kelly FJ. Plastic and human health: a micro issue? Environ Sci Technol. 2017;51(12):6634–6647. https://doi.org/10.1021/acs.est.7b00423
  • 2. Gall SC, Thompson RC. The impact of debris on marine life. Mar Pollut Bull. 2015;92(12):170–179. https://doi.org/10.1016/j.marpolbul.2014.12.041
  • 3. Rochman CM, Hoh E, Kurobe T, Teh SJ. Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress. Sci Rep. 2013;3:3263. https://doi.org/10.1038/srep03263
  • 4. Waring RH, Harris RM, Mitchell SC. Plastic contamination of the food chain: A threat to human health? Maturitas. 2018;115:64–68. https://doi.org/10.1016/j.maturitas.2018.06.010
  • 5. Rillig MC. Microplastic in terrestrial ecosystems and the soil? Environ Sci Technol. 2012;46(12):6453–6454. https://doi.org/10.1021/es302011r
  • 6. Food and Agriculture Organization. Assessment of agricultural plastics and their sustainability: A call for action. Rome: FAO; 2021. https://doi.org/10.4060/cb7856en
  • 7. PlasticsEurope. Plastics – the Fast Facts. 2024. Available from: https://plasticseurope.org/knowledge-hub/plastics-the-fast-facts-2024/ 8. PlasticsEurope. The Circular Economy for Plastics – A European Analysis. 2024. Available from: https://plasticseurope.org/knowledge-hub/the-circular-economy-for-plastics-a-european-analysis-2024/ 9. WWF Türkiye. Plastik Atıkların Yönetimi: Türkiye Raporu. 2019. Available from: https://www.wwf.org.tr/
  • 10. Hahladakis JN, Velis CA, Weber R, Iacovidou E, Purnell P. An overview of chemical additives present in plastics: Migration, release, fate and environmental impact during their use, disposal and recycling. J Hazard Mater. 2018;344:179-199. https://doi.org/10.1016/j.jhazmat.2017.10.014
  • 11. Ellen MacArthur Foundation. Towards the Circular Economy: Economic and Business Rationale for an Accelerated Transition. 2013. Cowes, UK: Ellen MacArthur Foundation.
  • 12. WRAP. A Roadmap to 2025 - The UK Plastics Pact. 2018. Available from: https://www.wrap.org.uk/content/the-uk-plastics-pact-roadmap-2025
  • 13. Defra UK. Guidance on applying the Waste Hierarchy. 2011. Available from : https://www.gov.uk/government/publications/guidance-on-applying-the-waste-hierarchy
  • 14. Ellen MacArthur Foundation. The Global Commitment 2024 Progress Report. 2014. Available from: https://www.ellenmacarthurfoundation.org/global-commitment-2024/overview
  • 15. García-Depraect O, Bordel S, Lebrero R, Santos-Beneit F, Börner RA, Börner T, Muñoz R. Inspired by nature: Microbial production, degradation and valorization of biodegradable bioplastics for life-cycle-engineered products. Biotechnol Adv. 2021;53:107772. https://doi.org/10.1016/j.biotechadv.2021.107772
  • 16. Highmoore JF, Kariyawasam LS, Trenor SR, Yang Y. Design of depolymerizable polymers toward a circular economy. Green Chem. 2024;26:(5):2384–2420. https://doi.org/10.1039/D3GC04215D
  • 17. Carbios. Carbios: A Circular Economy for Plastics. Carbios. 2024. Available from: https://www.carbios.com/en/
  • 18. Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, et al. A bacterium that degrades and assimilates poly(ethylene terephthalate). Science. 2016;351(6278):1196–1199. https://doi.org/10.1126/science.aad6359 19. Danso D, Chow J, Streit WR. Plastics: Environmental and biotechnological perspectives on microbial degradation. Appl Environ Microbiol. 2019;85(19):e01095–19. https://doi.org/10.1128/AEM.01095-19 20. Wei R, Zimmermann W. Microbial enzymes for the recycling of recalcitrant petroleum‐based plastics: How far are we? Microb Biotechnol. 2017;10(6):1308–1322. https://doi.org/10.1111/17517915.12710 21. Vona IA, Costanza JR, Cantor HA, Robert WJ. Manufacture of plastics. Wiley, New York. 1965:1(66):141–142.
  • 22. Shah AA, Hasan F, Hameed A, Ahmed S. Biological degradation of plastics:A comprehensive review. Biotechnol Adv. 2008;26:246–265. https://doi:10.1016/j.biotechadv.2007.12.005
  • 23. Tokiwa Y, Calabia BP, Ugwu CU, Aiba S. Biodegradability of Plastics. Int J Mol Sci. 2009;10:3722–3742. https://doi.org/10.3390/ijms10093722
  • 24. Gross RA, Kalra B. Biodegradable polymers for the environment. Science. 2002;297(5582):803–807. https://doi.org/10.1126/science.297.5582.803
  • 25. Williams DF. Enzymic hydrolysis of polylactic acid. Eng Med. 1981;10(1):5–7. https://doi.org/10.1243/EMED_JOUR_1981_010_004
  • 26. Montazer Z, Habibi Najafi MB, Levin DB. Challenges with verifying microbial degradation of polyethylene. Polymers. 2020;12:123. https://doi.org/10.3390/polym12010123
  • 27. Mierzwa-Hersztek M., Gondek K., Kopeć M. Degradation of polyethylene and biocomponent-derived polymer materials: An overview. J Polym Environ. 2019;27:600–611. https://doi.org/10.1007/s10924-019-01368-4
  • 28. Kaushal J, Khatri M, Arya S. Recent insight into enzymatic degradation of plastics prevalent in the environment: A mini - review. Clean Eng Technol. 2021;2:100083. https://doi.org/10.1016/j.clet.2021.100083
  • 29. Sang T, Wallis CJ, Hill G, Britovsek GJ. Polyethylene terephthalate degradation under natural and accelerated weathering conditions. Eur Polym J. 2020;136:109873. https://doi.org/10.1016/j.eurpolymj.2020.109873
  • 30. Raheem AB, Noor ZZ, Hassan A, Abd Hamid MK, Samsudin SA, Sabeen AH. Current developments in chemical recycling of post-consumer polyethylene terephthalate wastes for new materials production: A review. J Clean Prod. 2019;225:1052–1064. https://doi.org10.1016/j.jclepro.2019.04.019
  • 31. Zimmermann W, Billig S. Enzymes for the biofunctionalization of poly(ethylene terephthalate). Adv Biochem Eng Biotechnol. 2011;125:97–120. https://doi.org/10.1007/10_2010_87
  • 32. Fischer-Colbrie G, Heumann S, Liebminger S, Almansa E, Cavaco-Paulo A, Guebitz GM. New enzymes with potential for PET surface modification. Biocatal Biotransform. 2004;22:341–346. https://doi.org/10.1080/10242420400024565
  • 33. Chandramouli Swamy TM, Nagarathna SV, Reddy PV, Nayak AS. Efficient biodegradation of Polyethylene terephthalate (PET) plastic by Gordonia sp. CN2K isolated from plastic contaminated environment. Ecotoxicol Environ Saf. 2024;281:116635. https://doi.org/10.1016/j.ecoenv.2024.116635
  • 34. Torena P, Alvarez-Cuenca M, Reza M. Biodegradation of polyethylene terephthalate microplastics by bacterial communities from activated sludge. Can J Chem Eng. 2021;99(S1):S69–S82. https://doi.org/10.1002/cjce.24015
  • 35. Yip A, McArthur OD, Ho KC, Aucoin MG, Ingalls BP. Degradation of polyethylene terephthalate (PET) plastics by wastewater bacteria engineered via conjugation. Microb Biotechnol. 2024;17:e70015. https://doi.org/10.1111/1751-7915.70015
  • 36. Roman EKB, Ramos MA, Tomazetto G, Foltran BB, Galvão MH, Ciancaglini I, et al. Plastic-degrading microbial communities reveal novel microorganisms, pathways, and biocatalysts for polymer degradation and bioplastic production. Sci Total Environ. 2024;949:174876. https://doi.org/10.1016/j.scitotenv.2024.174876 37. Carniel A, Valoni É, Junior JN, Gomes A, Castro AM. Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid. Process Biochem. 2017;59:84–90. https://doi.org/10.1016/j.procbio.2016.07.023
  • 38. Rowe L, Howard GT. Growth of Bacillus subtilis on polyurethane and the purification and characterization of a polyurethanase-lipase enzyme. Int Biodeterior Biodegrad. 2002;50:33–40. https://doi.org/10.1016/S0964-8305(02)00047-1
  • 39. Nakkabi A, Sadiki M, Fahim M, Ittobane N, Ibnsouda Koraichi S, Barkai H, et al. Biodegradation of poly(ester urethane)s by Bacillus subtilis. Int J Environ Res. 2015;9(1):157–162
  • 40. Razak NASA, Habib S, Shukor MYA, Alias SA, Smykla J, Yasid NA. Isolation and Characterisation of Polypropylene Microplastic-Utilising Bacterium from the Antarctic Soil. arXiv. 2024. https://doi.org/10.48550/arXiv.2401.02096
  • 41. Peng BY, Chen Z, Chen J, Yu H, Zhou X, Criddle CS, et al. Biodegradation of polyvinyl chloride (PVC) in Tenebrio molitor (Coleoptera: Tenebrionidae) larvae. Environ Int. 2020;145:106106. https://doi.org/10.1016/j.envint.2020.106106
  • 42. Sadat-Shojai M, Bakhshandeh GR. Recycling of PVC wastes. Polym Degrad Stab. 2011;96:404–415. https://doi.org/10.1016/j.polymdegradstab.2010.12.001
  • 43. Winkler DE. Mechanism of polyvinyl chloride degradation and stabilization. J Polym Sci. 1959;35:3–16. https://doi.org/10.1002/pol.1959.1203512802
  • 44. Pospíšil J, Horák Z, Kruliš Z, Nešpůrek S, Kuroda SI. Degradation and aging of polymer blends I. Thermomechanical and thermal degradation. Polym Degrad Stab. 1999;65:405–414. https://doi.org/10.1016/S0141-3910(99)00029-4
  • 45. Zhang Z, Peng H, Yang D, Zhang G, Zhang J, Ju F. Polyvinyl chloride degradation by a bacterium isolated from the gut of insect larvae. Nat Commun. 2022;13(1):5360. doi:10.1038/s41467-022-32903-y
  • 46. Mohanan N, Montazer Z, Sharma PK, Levin DB. Microbial and enzymatic degradation of synthetic plastics. Front Microbiol. 2020;11:2837. https://doi.org/10.3389/fmicb.2020.580709
  • 47. Chaudhary AK, Vijayakumar RP. Studies on biological degradation of polystyrene by pure fungal cultures. Environ Dev Sustain. 2020;22:4495–4508. https://doi.org/10.1007/s10668-019-00394-5
  • 48. Ho BT, Roberts TK, Lucas S. An overview on biodegradation of polystyrene and modified polystyrene: The microbial approach. Crit Rev Biotechnol. 2017;38:308–320. https://doi.org/10.1080/07388551.2017.1355293
  • 49. Krueger MC, Seiwert B, Prager A, Zhang S, Abel B, Harms H, Schlosser D. Degradation of polystyrene and selected analogues by biological Fenton chemistry approaches: Opportunities and limitations. Chemosphere. 2017;173:520–528 https://doi.org/10.1016/j.chemosphere.2017.01.089
  • 50. Kim HR, Koh HY, Shin H, Suh DE, Lee S, Choi D. Enhancing the oxidation of polystyrene through a homogeneous liquid degradation system for effective microbial degradation. Front Microbiol. 2024;15:1509603. https://doi.org/10.3389/fmicb.2024.1509603
  • 51. Wang J, Liu R, Zhao S, Zhang B, Shao Z. Construction of an efficient polystyrene-degrading microbial consortium based on degrading and non-degrading bacteria predominant in biofilms of marine plastic debris. Front Mar Sci. 2025;12. https://doi.org/10.3389/fmars.2025.1569583
  • 52. Itävaara M, Karjomaa S, Selin JF. Biodegradation of polylactide in aerobic and anaerobic thermophilic conditions. Chemosphere. 2002;46:879–885. https://doi.org/10.1016/S0045-6535(01)00163-1
  • 53. Cui L, Wang X, Szarka G, Hegyesi N, Wang Y, Sui X, et al. Quantitative analysis of factors determining the enzymatic degradation of poly(lactic acid). Int J Biol Macromol. 2022;209:1703–1709. https://doi.org/10.1016/j.ijbiomac.2022.04.121
  • 54. Shin N, Kim SH, Oh J, Kim S, Lee Y, Shin Y, Choi S, Bhatia SK, Kim Y-G, Yang Y-H. Reproducible Polybutylene Succinate (PBS)-Degrading Artificial Consortia by Introducing the Least Type of PBS-Degrading Strains. Polymers. 2024;16(5):651. https://doi.org/10.3390/polym16050651 55. Howard GT. Biodegradation of polyurethane: A review. Int Biodeterior Biodegrad. 2002;49:245–252. https://doi.org/10.1016/S0964-8305(02)00051-3
  • 56. Álvarez-Barragán J, Domínguez-Malfavón L, Vargas-Suárez M, González-Hernández R, Aguilar-Osorio G, Loza-Tavera H. Biodegradative activities of selected environmental fungi on a polyester polyurethane varnish and polyether polyurethane foams. Appl Environ Microbiol. 2016;82:5225–5235. https://doi.org/10.1128/AEM.01344-16
  • 57. Nakajima-Kambe T, Shigeno-Akutsu Y, Nomura N, Onuma F, Nakahara T. Microbial degradation of polyurethane, polyester polyurethanes and polyether polyurethanes. Appl Microbiol Biotechnol. 1999;51:134–140. https://doi.org/10.1007/s002530051373
  • 58. Ru J, Chen X, Dong X, Hu L, Zhang J, Yang Y. Discovery of a polyurethane-degrading enzyme from the gut bacterium of plastic-eating mealworms. J Hazard Mater. 2024;480:136159. https://doi.org/10.1016/j.jhazmat.2024.136159
  • 59. Al Hosni AS, Pittman JK, Robson GD. Microbial degradation of four biodegradable polymers in soil and compost demonstrating polycaprolactone as an ideal compostable plastic. Waste Manag. 2019;97:105–114. https://doi.org/10.1016/j.wasman.2019.07.042
  • 60. Geyer R, Jambeck JR, Law KL. Production, use, and fate of all plastics ever made. Sci Adv. 2017;3:1700782. https://doi.org/10.1126/sciadv.1700782
  • 61. Badino SF, Bååth JA, Borch K, Jensen K, Westh P. Adsorption of enzymes with hydrolytic activity on polyethylene terephthalate. Enzyme Microb Technol. 2021;152:109937. https://doi.org/10.1016/j.enzmictec.2021.109937
  • 62. Taghavi N, Zhuang WQ, Baroutian S. Enhanced biodegradation of non-biodegradable plastics by UV radiation: Part 1. J Environ Chem Eng. 2021;9:106464. https://doi.org/10.1016/j.jece.2021.106464
  • 63. Vedrtnam A, Kumar S, Chaturvedi S. Experimental study on mechanical behavior, biodegradability, and resistance to natural weathering and ultraviolet radiation of wood-plastic composites. Compos B Eng. 2019;176:107282. https://doi.org/10.1016/j.compositesb.2019.107282
  • 64. Arutchelvi J, Sudhakar M, Arkatkar A, Doble M, Bhaduri S, Uppara PV. Biodegradation of polyethylene and polypropylene. IJBT. 2008;7(1):9–22
  • 65. Amobonye A, Bhagwat P, Singh S, Pillai S. Plastic biodegradation: Frontline microbes and their enzymes. Sci Total Environ. 2021;759:143536. https://doi.org/10.1016/j.scitotenv.2020.143536
  • 66. Yuan J, Ma J, Sun Y, Zhou T, Zhao Y, Yu F. Microbial degradation and other environmental aspects of microplastics/plastics. Sci Total Environ. 2020;715:136968. https://doi.org/10.1016/j.scitotenv.2020.136968
  • 67. Wang GX, Huang D, Ji JH, Völker C, Wurm FR. Seawater-Degradable Polymers-Fighting the Marine Plastic Pollution. Adv Sci. 2020;8:2001121. https://doi.org/10.1002/advs.202001121
  • 68. Emadian SM, Onay TT, Demirel B. Biodegradation of bioplastics in natural environments. Sci Total Environ. 2017;574:1079-92. https://doi.org/10.1016/j.scitotenv.2016.09.162
  • 69. Chigwada AD, Tekere M. The plastic and microplastic waste menace and bacterial biodegradation for sustainable environmental clean-up: a review. Environ Res. 2023;10:116110. https://doi.org/10.1016/j.envres.2023.116110
  • 70. Sarsan S, Kodaparthi A, Susmitha B. Microbial enzymes in plastic degradation. In Developments in Applied Microbiology and Biotechnology. Microbial Essentialism. 2024; 207–242. https://doi.org/10.1016/b978-0-443-13932-1.00005-2
  • 71. Temporiti MEE, Nicola L, Nielsen E, Tosi S. Fungal Enzymes Involved in Plastics Biodegradation. Microorganisms. 2022;10(6):1180. Published 2022 Jun 8. https://doi.org/10.3390/microorganisms10061180
  • 72. Glaser JA. Biological degradation of polymers in the environment. In: Kästner M, Trapp S, editors. Plastics in the Environment. London: IntechOpen; 2019. p.13–30. https://doi.org/10.5772/intechopen.85124
  • 73. Vaishnav A, Lal J, Singh N, Pati BK, Mehta NK, Priyadarshini MB. Role of microbial enzymes and their modification for plastic biodegradation. In: Advanced Strategies for Biodegradation of Plastic Polymers. Springer. 2024;373–403. https://doi.org/10.1007/978-3-031-55661-6_16
  • 74. Asiandu AP, Wahyudi A, Sari SW. A review: plastics waste biodegradation using plastics-degrading bacteria. J Environ Treat Tech. 2021;9(1):148–157. https://doi.org/10.47277/JETT/9(1)157
  • 75. Srikanth M, Sandeep TSRS, Sucharitha K, Godi S. Biodegradation of plastic polymers by fungi: a brief review. Bioresour Bioprocess. 2022;9(1):42. https://doi.org/10.1186/s40643-022-00532-4
  • 76. Albertsson AC, Karlsson S. Aspects of biodeterioration of inert and degradable polymers. Int Biodeterior Biodegrad. 1993;31:161–170. https://doi.org/10.1016/0964-8305(93)90002-J
  • 77. Ammala A, Bateman S, Deana K, Petinakis E, Sangwan P, Wong S, et al. An overview of degradable and biodegradable polyolefins. Prog Polym Sci. 2011;36:1015–1049. https://doi.org/10.1016/j.progpolymsci.2010.12.002
  • 78. Fesseha H, Abebe F. Degradation of plastic materials using microorganisms: A review. Public Health Open J. 2019;4(2):57–63. https://doi.org/10.17140/PHOJ-4-136
  • 79. Urbanek AK, Mirończuk AM, García-Martín A, Saborido A, de la Mata I, Arroyo M. Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester type plastics. Biochim Biophys Acta Proteins Proteom. 2020;1868(2):140315. https://doi.org/10.1016/j.bbapap.2019.140315
  • 80. Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, et al. An engineered PET depolymerase to break down and recycle plastic bottles. Nature. 2020;580(7802):216–219. https://doi.org/10.1038/s415860202149-4
  • 81. Kale G, Kijchavengkul T, Auras R, Rubino M, Selke SE, Singh SP. Compostability of bioplastic packaging materials: an overview. Macromol Biosci. 2007;7(3):255–277. https://doi.org/10.1002/mabi.200600168
  • 82. Narancic T, Verstichel S, Reddy Chaganti S, et al. Biodegradable plastic blends create new possibilities for end of life management of plastics but they are not a panacea for plastic pollution. Environ Sci Technol. 2018;52(18):10441–10452. https://doi.org/10.1021/acs.est.8b02963
  • 83. Egmond MR, de Vlieg J. Fusarium solani pisi cutinase. Biochimie. 2000;82(11):1015–1021. https://doi.org/10.1016/s0300-9084(00)01183-4
  • 84. Chen S, Su L, Chen J, Wu J. Cutinase: characteristics, preparation and application. Biotechnol Adv. 2013;31:1754–1767. https://doi.org/10.1016/j.biotechadv.2013.09.005
  • 85. Sharma AK, Tiwari RP, Hoondal GS. Properties of a thermostable and solvent stable extracellular lipase from a Pseudomonas sp. AG-8. J Basic Microbiol. 2001;41(6):363–366. https://doi.org/10.1002/1521-4028(200112)41:6<363::AID-JOBM363>3.0.CO;2-C
  • 86. Houde A, Kademi A, Leblanc D. Lipases and their industrial applications: An overview. Appl Biochem Biotechnol. 2004;118(1–3):155–170. https://doi.org/10.1385/abab:118:1-3:155
  • 87. Purdy RE, Kolattukudy PE. Hydrolysis of plant cuticle by plant pathogens. Properties of cutinase I, cutinase II, and a nonspecific esterase isolated from Fusarium solani pisi. Biochemistry. 1975;14(13):2832–2840. https://doi.org/10.1021/bi00684a007
  • 88. Dutta K, Sen S, Veeranki VD. Production, characterization and applications of microbial cutinases. Process Biochem. 2009;44:127–134. https://doi.org/10.1016/j.procbio.2008.09.008
  • 89. Alisch M, Feuerhack A, Müller H, Mensak B, Andreaus J, Zimmermann W. Biocatalytic modification of polyethylene terephthalate fibres by esterases from actinomycete isolates. Biocatal Biotransform. 2004;22(5–6):347–351. https://doi.org/10.1080/10242420400025877
  • 90. Wang G, Guo Z, Zhang X, Wu H, Bai XM, Zhang H, et al. Heterologous expression of pediocin/papA in Bacillus subtilis. Microb Cell Fact. 2022;21:104. https://doi.org/10.1186/s12934-022-01829-x
  • 91. Maurya A, Bhattacharya A, Khare SK. Enzymatic remediation of polyethylene terephthalate (PET)-based polymers for effective management of plastic wastes: An overview. Front Bioeng Biotechnol. 2020;8:602325. https://doi.org/10.3389/fbioe.2020.602325
  • 92. Baker PJ, Poultney C, Liu Z, Gross R, Montclare JK. Identification and comparison of cutinases for synthetic polyester degradation. Appl Microbiol Biotechnol. 2012;93(1):229–240. https://doi.org/10.1007/s00253-011-3402-4
  • 93. Hellesnes KN, Vijayaraj S, Fojan P, Petersen E, Courtade G. Biochemical characterization and NMR study of a PET-Hydrolyzing cutinase from Fusarium solani pisi. Biochemistry. 2023;62(8):1369–1375. https://doi.org/10.1021/acs.biochem.2c00619
  • 94. de Castro AM, Carniel A, Nicomedes Junior J, da Conceição Gomes A, Valoni É. Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources. J Ind Microbiol Biotechnol. 2017;44(6):835–844. https://doi.org/10.1007/s10295-017-1942-z
  • 95. Dimarogona M, Nikolaivits E, Kanelli M, Christakopoulos P, Sandgren M, Topakas E. Structural and functional studies of a Fusarium oxysporum cutinase with polyethylene terephthalate modification potential. Biochim Biophys Acta. 2015;1850(11):2308–2317. https://doi.org/10.1016/j.bbagen.2015.08.009
  • 96. Ronkvist ÅM, Xie W, Lu W, Gross RA. Cutinase-catalyzed hydrolysis of poly (ethylene terephthalate). Macromolecules. 2009;42:5128–5138. https://doi.org/10.1021/ma9005318
  • 97. Murphy CA, Cameron JA, Huang SJ, Vinopal RT. Fusarium polycaprolactone depolymerase is cutinase. Appl Environ Microbiol. 1996;62(2):456–460. https://doi.org/10.1128/aem.62.2.456–460.1996
  • 98. Adıgüzel AO, Tunçer M. Purification and characterization of cutinase from Bacillus sp. KY0701 isolated from plastic wastes. Prep Biochem Biotechnol. 2017;47:925–933. https://doi.org/10.1080/10826068.2017.1365245 99. Ho BT, Roberts TK, Lucas S. An overview on biodegradation of polystyrene and modified polystyrene: the microbial approach. Crit Rev Biotechnol. 2018;38:308–320. https://doi.org/10.1080/07388551.2017.1355293
  • 100. Hu X, Gao Z, Wang Z, Su T, Yang L, Li P. Enzymatic degradation of poly (butylene succinate) by cutinase cloned from Fusarium solani. Polym Degrad Stab. 2016;134:211–219. https://doi.org/10.1016/j.polymdegradstab.2016.10.012
  • 101. Van Gemeren IA, Beijersbergen A, van den Hondel CA, Verrips CT. Expression and secretion of defined cutinase variants by Aspergillus awamori. Appl Environ Microbiol. 1998;64(8):2794–2799. https://doi.org/10.1128/AEM.64.8.2794-2799.1998
  • 102. Phytian SJ. Esterases. In: Kelly DR, editor. Biotechnology. 2nd ed. Weinheim: Wiley-VCH; 1998:193–241.
  • 103. Zhang L, Cao K, Liu H, Wang Y, Zhang B, Han H, et al. Discovery of a polyester polyurethane-degrading bacterium from a coastal mudflat and identification of its degrading enzyme. J Hazard Mater. 2025;483:136659. https://doi.org/10.1016/j.jhazmat.2024.136659
  • 104. Lin J, Sun K, Ma L, Li C, Tong H, Wang Z. Enzymatic degradation of polybutylene succinate by recombinant cutinase cloned from Paraphoma chrysanthemicola. J Environ Manage. 2025;375:124288. https://doi.org/10.1016/j.jenvman.2025.124288
  • 105. Bornscheuer UT, Kazlauskas RJ. Hydrolases in organic synthesis: regio- and stereoselective biotransformations. 1999. Wiley-VCH, Weinheim.
  • 106. Hoshino A, Isono Y. Degradation of aliphatic polyester films by commercially available lipases with special reference to rapid and complete degradation of poly(L-lactide) film by lipase PL derived from Alcaligenes sp. Biodegradation. 2002;13(2):141–147. https://doi.org/10.1023/a:1020450326301
  • 107. Safdar A, Ismail F, Imran M. Biodegradation of synthetic plastics by the extracellular lipase of Aspergillus niger. Environ Adv. 2024;17:100563. https://doi.org/10.1016/j.envadv.2024.100563.
  • 108. Kawai F, Kawabata T, Oda M. Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields. Appl Microbiol Biotechnol. 2019;103(11):4253–4268. https://doi.org/10.1007/s00253-019-09717-y
  • 109. Khairul Anuar NFS, Huyop F, Ur-Rehman G, Abdullah F, Normi YM, Sabullah MK, et al. An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation. Int J Mol Sci. 2022;23(20):12644. https://doi.org/10.3390/ijms232012644
  • 110. Vidal P, Martínez-Martínez M, Fernandez-Lopez L, Roda S, Méndez-García C, Golyshina OV, et al. Metagenomic mining for esterases in the microbial community of los rueldos acid mine drainage formation. Front Microbiol. 2022;13:868839. https://doi.org/10.3389/fmicb.2022.868839
  • 111. Khan S, Nadir S, Shah ZU, Shah AA, Karunarathna SC, Xu J, et al. Biodegradation of polyester polyurethane by Aspergillus tubingensis. Environ Pollut. 2017;225:469–480. https://doi.org/10.1016/j.envpol.2017.03.012
  • 112. Brunner I, Fischer M, Rüthi J, Stierli B, Frey B. Ability of fungi isolated from plastic debris floating in the shoreline of a lake to degrade plastics. PLoS One. 2018;13(8):e0202047. https://doi.org/10.1371/journal.pone.0202047
  • 113. Barclay A, Acharya KR. Engineering plastic eating enzymes using structural biology. Biomolecules. 2023;13(9):1407. https://doi.org/10.3390/biom13091407
  • 114. Chen CC, Han X, Ko TP, Liu W, Guo RT. Structural studies reveal the molecular mechanism of PETase. FEBS J. 2018;285(20):3717-3723. https://doi.org/10.1111/febs.14612
  • 115. Kawai F, Kawabata T, Oda M. Current state and perspectives related to the polyethylene terephthalate hydrolases available for biorecycling. ACS Sustain Chem Eng. 2020;8:8894–8908. https://doi.org/10.1021/acssuschemeng.0c01638
  • 116. Palm GJ, Reisky L, Böttcher D, Müller H, Michels EAP, Walczak MC, et al. Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate. Nat Commun. 2019;10(1):1717. https://doi.org/10.1038/s41467-019-09326-3
  • 117. Han Z, Nina MRH, Zhang X, Huang H, Fan D, Bai Y. Discovery and characterization of two novel polyethylene terephthalate hydrolases: one from a bacterium identified in human feces and one from the Streptomyces genus. J Hazard Mater. 2024;472:134532. https://doi.org/10.1016/j.jhazmat.2024.134532
  • 118. Mohanan N, Montazer Z, Sharma PK, Levin DB. Microbial and enzymatic degradation of synthetic plastics. Front Microbiol. 2020;11:580709. https://doi.org/10.3389/fmicb.2020.580709
  • 119. Furukawa M, Kawakami N, Oda K, Miyamoto K. Acceleration of Enzymatic Degradation of Poly(ethylene terephthalate) by Surface Coating with Anionic Surfactants. ChemSusChem. 2018;11(23):4018-4025. https://doi.org/10.1002/cssc.201802096
  • 120. Ikehata K, Buchanan ID, Smith DW. Recent developments in the production of extracellular fungal peroxidases and laccases for waste treatment. J Environ Eng Sci. 2004;3:1–19. https://doi.org/10.1139/s03-077 121. Hofrichter M, Ullrich R. Heme-thiolate haloperoxidases: versatile biocatalysts with biotechnological and environmental significance. Appl Microbiol Biotechnol. 2006;71(3):276–288. https://doi.org/10.1007/s00253-006-0417-3
  • 122. Conesa A, Punt PJ, van den Hondel CA. Fungal peroxidases: Molecular aspects and applications. J Biotechnol. 2002;93:143–158. https://doi.org/10.1016/S0168-1656(01)00394-7
  • 123. Maciel MJM, Ribeiro HCT. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Electron J Biotechnol. 2010;13:14–15. https://doi.org/10.2225/vol13-issue6-fulltext-2
  • 124. Iiyoshi Y, Tsutsumi Y, Nishida T. Polyethylene degradation by lignin-degrading fungi and manganese peroxidase. J Wood Sci. 1998;44:222–229. https://doi.org/10.1007/BF00521967
  • 125. Ganesh P, Dineshraj D, Yoganathan K. Production and screening of depolymerising enzymes by potential bacteria and fungi isolated from plastic waste dump yard sites. Int J Appl Res. 2017;3(3):693–695
  • 126. Olivieri G, Wijffels RH, Marzocchella A, Russo ME. Bioreactor and bioprocess design issues in enzymatic hydrolysis of lignocellulosic biomass. Catalysts. 2021;11:680. https://doi.org/10.3390/catal11060680
  • 127. Muangchinda C, Pinyakong O. Enrichment of LDPE-degrading bacterial consortia: Community succession and enhanced degradation efficiency through various pretreatment methods. Sci Rep. 2024;14(1):28795. https://doi.org/10.1038/s41598-024-80306-4
  • 128. Cazaudehore G, Guyoneaud R, Vasmara C, Greuet P, Gastaldi E, Marchetti R, et al. Impact of mechanical and thermo-chemical pretreatments to enhance anaerobic digestion of poly(lactic acid). Chemosphere. 2022;297:133986. https://doi.org/10.1016/j.chemosphere.2022.133986
  • 129. Arkatkar A, Arutchelvi J, Bhaduri S, Uppara PV, Doble M. Degradation of unpretreated and thermally pretreated polypropylene by soil consortia. Int Biodeterior Biodegrad. 2009;63(1):106–111. https://doi.org/10.1016/j.ibiod.2008.06.005
  • 130. Edge M, Hayes M, Mohammadian M, Allen NS, Jewitt TS, Brems K, et al. Aspects of poly(ethylene terephthalate) degradation for archival life and environmental degradation. Polym Degrad Stab. 1991;32(2):131–153. https://doi.org/10.1016/0141-3910(91)90047-U
  • 131. Rostampour S, Cook R, Jhang SS, Li Y, Fan C, Sung LP. Changes in the Chemical Composition of Polyethylene Terephthalate under UV Radiation in Various Environmental Conditions. Polymers. 2024;16(16):2249. https://doi.org/10.3390/polym16162249
  • 132. Jiang Z, Chen X, Xue H, Li Z, Lei J, Yu M, et al. Novel polyurethane-degrading cutinase BaCut1 from Blastobotrys sp. G-9 with potential role in plastic bio-recycling. J Hazard Mater. 2024;472:134493. https://doi.org/10.1016/j.jhazmat.2024.134493
  • 133. Li S, Zhang, W. Computational identification of plastic-degrading enzymes in ocean microbiomes. Sci Rep. 2025;15:15332. https://doi.org/10.1038/s41598-025-99275-3
  • 134. Zhang Y, Wang Y, Wang B, Xia X, Wang T, Lu Y. Mild PET degradation by enzymes coupled with magnetic and optical manipulation. J Hazard Mater. 2025;494:138663. https://doi.org/10.1016/j.jhazmat.2025.138663 135. Shi K, Su T, Wang Z. Comparison of poly(butylene succinate) biodegradation by Fusarium solani cutinase and Candida antarctica lipase. Polym Degrad Stab. 2019;164:55–60. https://doi.org/10.1016/j.polymdegradstab.2019.04.005
  • 136. Herrero Acero E, Ribitsch D, Dellacher A, Zitzenbacher S, Marold A, Steinkellner G, et al. Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis. Biotechnol Bioeng. 2013;110(10):2581–2590. https://doi.org/10.1002/bit.24930 137. Ribitsch D, Herrero Acero E, Greimel KJ, Eiteljörg I, Trotscha E, Freddi G, et al. Characterization of a new cutinase from Thermobifida alba for PET-surface hydrolysis. Biocatal Biotransform. 2012;30(1):2–9. https://doi.org/10.3109/10242422.2012.644435
  • 138. Din SU, Kalsoom, Satti SM, Uddin S, Mankar SV, Ceylan E, et al. The purification and characterization of a cutinase-like enzyme with activity on polyethylene terephthalate (pet) from a newly ısolated bacterium Stenotrophomonas maltophilia PRS8 at a mesophilic temperature. Appl Sci. 2023;13(6):3686. https://doi.org/10.3390/app13063686
  • 139. Jung HW, Mei-Kwei Y, Su RC. Purification, characterization, and gene cloning of an Aspergillus fumigatus polyhydroxybutyrate depolymerase used for degradation of polyhydroxybutyrate, polyethylene succinate, and polybutylene succinate. Polym Degrad Stab. 2018;154:186–194. https://doi.org/10.1016/j.polymdegradstab.2018.06.002
  • 140. Gautam R, Bassi A, Yanful E. Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium. Biotechnology Letters. 2007;29:1081–6. https://doi.org/10.1007/s10529-007-9354-1
  • 141. Nechwatal A, Blokesch A, Nicolai M, Krieg M, Kolbe A, Wolf M, et al. A contribution to the investigation of enzyme catalysed hydrolysis of poly(ethylene terephthalate) oligomers. Macromol Mater Eng. 2006;291:1486–1494. https://doi.org/10.1002/mame.200600204 142. Akutsu Y, Nakajima-Kambe T, Nomura N, Nakahara T. Purification and Properties of a Polyester Polyurethane-Degrading Enzyme from Comamonas acidovorans TB-35. Appl Environ Microbiol. 1998;64(1):62–67. https://doi.org/10.1128/AEM.64.1.62-67.1998
Toplam 130 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Biyokataliz ve Enzim Teknolojisi, Endüstriyel Mikrobiyoloji
Bölüm Derleme
Yazarlar

Sermin Yıldırım 0000-0002-9785-3282

Gönderilme Tarihi 18 Haziran 2025
Kabul Tarihi 18 Temmuz 2025
Yayımlanma Tarihi 29 Temmuz 2025
IZ https://izlik.org/JA85EJ46UY
Yayımlandığı Sayı Yıl 2025 Cilt: 1 Sayı: 2

Kaynak Göster

Vancouver 1.Sermin Yıldırım. Plastic Biodegradation: Potential of Microbial Enzymes. SABİB [Internet]. 01 Temmuz 2025;1(2):57-78. Erişim adresi: https://izlik.org/JA85EJ46UY