İndometazin ile Gastrik Hasar Oluşturulmuş Sıçanların Mide Dokusunda AOPP Ve Total Sülfidril Düzeyi Üzerine Tarantula Cubensis Alkolik Ekstraktı’nın Etkisi

Öz

Tarantula cubensis örümceğinin alkolik ekstraktı (TCAE) veteriner sahada homeopatik ilaç olarak kullanılmaktadır. TCAE antiinflamatuar, antitümör, nekrotizan etki ve yara iyileşmesi gibi birçok terapötik etkiye sahiptir. Hücrenin oksidatif stres durumunu değerlendirmek için kullanılan birçok belirteç vardır. Oksijen radikallerinin hücredeki proteinlerle reaksiyonu ile oluşan ilerlemiş protein oksidasyon ürünleri ‘advanced oxidative protein products’ (AOPP) bu belirteçlerden biridir. Total sülfidril (TSG) ise antioksidan durumun göstergesi olarak kabul edilmektedir. Bu çalışmada indometazin ile gastrik hasar oluşturulmuş sıçanların mide dokusunda AOPP ve TSG düzeyi üzerine TCAE’nin etkisinin incelenmesi amaçlandı. Bu amaçla sıçanlar kontrol grubu, ülser grubu (25mg/kg indometazin), İndometazin + Lansoprazol (25mg/kg indometazin + 30 mg/kg lansoprazol) alan ülser grubu (referans anti-ülser ilaç), İndometazin + TCAE (25mg/kg indometazin + 200 µg/kg Theranekron) alan ülser grubu olmak üzere 4 gruba ayrıldı (n=7). Lansoprazol ve TCAE belirtilen dozlarda verildikten 5 dakika sonra negatif kontrol grubu hariç tüm sıçanlara aynı şekilde oral yolla tek doz indometazin verildi ve indometazin uygulamasından altı saat sonra anestezi altında mide dokusu biyokimyasal analiz için çıkarıldı. Mide dokusunda AOPP ve TSG düzeylerinin ölçümünde spektrofotometrik yöntem kullanıldı. Çalışmada, TCAE’nin etkileri ülser tedavisinde kullanılan lansoprazolun etkileri ile karşılaştırıldı. İndometazin grubunda AOPP ve TSG düzeyinde Kontrol grubuna göre istatistiksel olarak anlamlı (p<0.05) bir artış bulundu. TCAE uygulanan grupta AOPP ve TSG düzeyleri İndometazin ve İndometazin + Lansoprazol gruplarına göre daha düşük tespit edildi. Bu çalışmada TCAE uygulaması ile AOPP düzeyinde değişikliğin olmaması ve bunun sonucunda TSG düzeyinde herhangi bir artışın belirlenmemesi nedeniyle indometazin uygulaması sonucu oluşan gastrik hasara karşı TCAE’nin koruyucu bir rolünün olabileceği tespit edildi.

The Effect of Tarantula Cubensıs Alcoholic Extract on AOPP and Total Sulfhidryl Level in The Stomach Tissue of Gastric Damaged Rats with Indometazine

Öz

Alcoholic extract of the spider Tarantula Cubensis (TCAE) is used as a homeopathic agent in the Veterinary medicine. TCAE has many therapeutic effects such as anti-inflammatory, antitumor, necrotizing action and wound healing. There are many markers used to assess the oxidative stress state of the cell. Advanced oxidative protein products (AOPP), which are formed by the reaction of oxygen radicals with proteins in the cell, are one of these markers. Total sulfhydryl (TSG) is accepted as an indicator of antioxidant status. In this study, it was aimed to investigate the effect of TCAE on AOPP and TSG levels in the stomach tissue of rats with indomethacin-induced gastric damage. For this purpose, the rats were divided into 4 groups, including control group, ulcer group (25mg/kg indomethacin), Indomethacin + Lansoprazole (25mg/kg indomethacin + 30 mg/kg lansoprazole) ulcer group (reference anti-ulcer drug), Indomethacin + TCAE (25mg/kg indomethacin + 200 µg/kg Theranekron) ulcer group (n=7). 5 minutes after lansoprazole and TCAE were given at the indicated doses, all rats except the negative control group were given a single dose of indomethacin (25 mg/kg dose) orally in the same way, and six hours after indomethacin administration, the gastric tissue was removed for biochemical analysis under anesthesia. Spectrophotometric method was used to measure AOPP and TSG levels in gastric tissue. In the study, the effects of TCAE were compared with the effects of lansoprazole used in the treatment of ulcers. A statistically significant (p<0.05) increase was found in the AOPP and TSG levels in the indomethacin group compared to the control group. AOPP and TSG levels were found to be lower in the Indomethacin + TCAE group compared to the Indomethacin and Indomethacin + Lansoprazole groups. In this study, it was determined that TCAE may have a protective role against gastric damage caused by indomethacin administration, since there was no change in AOPP level with TCAE application and no increase in TSG level was detected as a result.

Anahtar Kelimeler

• Tarantula Cubensis Alcholic Extract
• Total Sulphidril
• İndomethacin
• Advanced oxidation protein products
• Gastric damage

Kaynakça

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