Analysis of Some Turkish Durum Wheat Cultivars and Landraces for HMW-Glutenin Subunits

Yıl 2019, , 74 - 80, 30.04.2019

Tuğba Eserkaya Güleç Ahmet Yıldırım Mehmet Koyuncu Abdulvahit Sayaslan

https://doi.org/10.13002/jafag4467

Cited By: 1

Öz

Gluten is a complex mixture of proteins composed mainly of glutenins and the gliadins. A strong correlation exists between the presence of certain gliadin and glutenin proteins and the viscoelastic properties of the gluten. For instance, HMW-glutenin subunits, encoded by the Glu-A1, Glu-B1 and Glu-D1 loci that are located on the long arms of chromosome 1, are strongly related to quality. This study were made for screening for the presence of HMW glutenin alleles of the durum wheat varieties grown in different regions of Turkey in 2012. In search, 17 Turkish durum wheat cultivars and 15 durum landraces and seven bread wheat cultivars as standards were used. This study was performed to determine the composition of HMW-glutenin subunits in gathered from different regions of Turkey. Five seeds from each cultivar were cut into two halves and one half was used for protein electrophoresis. The SDS-PAGE was performed to determine the composition of HMW-glutenin subunits. As a result, HMW-Glutenin alleles obtained from the SDS-PAGE screenings were compared with known LMW-Glutenins and -gliadin alleles determined in previous studies and the quality-related situations of the durum genotypes were assessed. As a result of the study, it was observed that 1 and 7+8 HMW-G subunits, which had a negative effect on quality when they were found together with durum wheat seeds, also included LMW-1-Glutenin and -gliadin 42. It has been determined that the varieties containing LMW-2 glutenin and -gliadin 45 which have a positive effect on the quality of pasta also include 7+9 HMW-Glutenin and 14+15 HMW-Glutenin subunits separately. It was determined that a single conclusion could not be reached regarding the effect of 6+8 HMW-Glutenin and 17+18 HMW-Glutenin subunits on quality.

Anahtar Kelimeler

Durum wheat, Triticum durum, HMW-Glutenin, LMW-Glutenin, -gliadin

Kaynakça

• Aalami M, Leelavathi K, Rao, UJSP. 2007. Spaghetti making potential of Indian durum wheat varieties in relation to their protein, yellow pigment and enzyme contents. Food Chemistry, 100: 1243-1248.
• Bekes F, Cavanagh CR, Martinov S, Bushuk W, Wrigley CW. 2011. The Gluten Composition of Wheat Varieties and Genotypes. AACC.
• Branlard G, Dardevet M. 1985. Diversity of grain protein and bread wheat quality. II. Correlation between high molecular weight subunits of glutenin and flour characteristic. J. Cere. Sci., 3: 345- 354.
• Branlard G, Autran JC, Monneveux P. 1989. High Molecular Weight glutenin subunits in makarnalık wheat. Theor. Appl. Genet., 78: 353-358.
• Brites CM, Carrillo JM, 2003. Inheritance of gliadin and glutenin proteins in four durum wheat crosses. Cereal Res. Comm., 28: 239-246.
• Butow BJ, Ma W, Gale KR, Cornish GB, Rampling L, Larroque O, Morell MK, Békés F. 2003. Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high-molecular-weight glutenin allele has a major impact on wheat flour dough strength. Theoretical and Applied Genetics., 107: 1524–1532.
• Fan X, Song Z, Kang H, Yang R., Zhou Y. 2009. Identification and Characterization of HMW-GSSubunits and Their Coding Sequences in Dwarfing Polish Wheat. International Journal of Agri. Research., 4: 237-249.
• Gianibelli MC, Lagudah ES, Wrigley CW, MacRitchie F. 2002. Biochemical and genetic characterization of a monomeric storage protein (T1) with an unusually high molecular weight in Triticum tauschii. Theorotical and Applied Genetics, 104: 497-504.
• Lukow OM, 1991. Screening of bread wheats for milling and baking quality- A Canadian perspective. Cereal Foods World, 36 (6): 497-501.
• Mohd S, Zeb A, Taufiq A, Ikhtiar K. 2007. Characterization of wheat varieties by seed storage-protein electrophoresis. African Journal of Bio. Vol., 6 (5): 497-500.
• Morris SR. 2004. Grain: Quality attributes. In: C. Wrigley, H. Corke, and C. Walker (Eds.), Encyclopedia of Grain Science, 238-254.
• Naghavi MR, Rashidi Monfared S, Ahkami AH, Ombidbakhsh MA. 2009. Genetic Variation of Durum Wheat Landraces and Cultivars Using Morphological and Protein Markers. World Academy of Science, Engineering and Technology, 49: 73-75.
• Oak MD, Tamhankar SA, Rao VS, Bhosale SB. 2004. Relationship of HMW, LMW Glutenin subunit and gliadin with gluten strenght in Indian Durum wheat. J. Plant Biochemistry and Biotechnology Vol., 13: 51-55.
• Payne PI, Lawrece GJ. 1983. Catalogue of alleles fort he complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular weight subunits of glutenin in hexaploid wheat. Cereal Res. Communs, 11: 29-35
• Payne PI, Holt LM, Lawrence GJ, Law CN. 1982. The genetic of gliadin and glutenin - The major storage proteins of the wheat endosperm. Plant Foods for Human Nutrition, 31: 229-241.
• Sayaslan A, Koyuncu M, Yıldırım A, Güleç ET, Sönmezoğlu AÖ, Kandemir N. 2012. Some quality characteristics of selected durum wheat (Triticum durum) landraces. Turkish Journal Agricaltural Forrest, 36: 749-756.
• Singh NK, Shepherd KW, Cornish GB. 1991. A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science, 14: 203-208.
• Sissons MJ, Ames NP, Hare RA, Clarke JM. 2005. Relationship between glutenin subunit composition. Journal of Science of Food and Agriculture, 85: 2445-2452.
• Sönmezoğlu AÖ, Yıldırım A, Güleç ET, Kandemir N, Sayaslan A, Koyuncu M. 2010. Molecular Breeding of Selçuklu-97 Durum Wheat Cultivar for Some Genes Affecting Pasta Quality. Journal of Applied Biological Sciences, 4 (3): 01-06.
• Troccoli A, Borrelli GM, DeVita P, Fares C, DiFonzo N. 2000. Durum wheat quality: A multidisciplinary concept. Journal of Cereal Science, 32: 99-113.
• Waines JG, Payne PI. 1987. Electrophoretic analysis of the highmolecular-weight glutenin subunits of Triticum monococcum, T. urartu, and the A genome of bread wheat (T. aestivum). Theor Appl Genet., 74: 71–6.
• Zarkti H, Ouabbou H, Taghouti M, Hilali A, Udupa M. 2010. Comparison of genetic diversity at prolamin loci in Moroccan durum wheat accessions. Plant Omics Journal, 3(5): 154-158.