Determination of an SSR Marker Set to Distinguish Genotypes of Different Tobacco Classes

Abstract

Tobacco is produced as cultivated old varieties or local varieties in Turkey. Seed exchanges among farmers who produce their own seeds lead to different names for the same genotypes in different regions or the same name for different genotypes over time. Thus, an efficient cultivar identification system is required in tobaccos grown in Turkey. This study was carried out to determine a marker set to differentiate different tobacco classes grown in Turkey. A total of 14 genotypes from different tobacco classes, eight local varieties and six registered cultivars, were screened using 21 simple sequence repeats (SSR) markers. Seventeen SSR markers produced polymorphic bands while four markers were monomorphic. Average allele number of the markers was 3.38 and PIC values of the markers ranged from 0.000 to 0.741. In dendrogram established using the marker data, genotypes were separated in four distinct cluster. Clusters were made of Virginia or semi-oriental, tombac, oriental and rustica type genotypes. Genetic fingerprinting analyses grouped the genotypes of similar use classes together. According to the results obtained a marker set of eight SSR markers could efficiently and reliably differentiated the tobacco genotypes of different classes.

Keywords

• Fingerprint analysis
• Microsatellite
• Nicotiana tabacum
• Polymorphism

Kaynakça

1. Anderson JA, Churchill GA, Autrique JE, Tanksley SD and Sorrells ME (1993). Optimizing parental selection for genetic linkage maps. Genome, 36: 181-186.
2. Arslan B and Okumuş A (2006). Genetic and geographic polymorphism of cultivated tobacco (Nicotiana tabacum) in Turkey. Russian Journal of Genetics, 42: 667-671.
3. Bindler G, van der Hoeven R, Gunduz I, Plieske J, Ganal M, Rossi L, Gadani F and Donini P (2007). A microsatellite marker-based linkage map of tobacco. Theoretical and Applied Genetics, 114: 341-349.
4. Bindler G, Plieske J, Bakaher N, Gunduz I, Ivanov N, van der Hoeven R, Ganal M and Donini P (2011). A high-density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development. Theoretical and Applied Genetics, 123: 219-230.
5. Davalieva K, Maleva I, Filipovski K, Spiroski O and Efremov GD (2010). Genetic variability of Macedonian tobacco varieties determined by microsatellite marker analysis. Diversity, 2: 439-449.
6. Darvishzadeh RL, Mirzaei HH, Maleki H, Laurentin S and Alavi R (2013). Genetic variation in oriental tobacco (Nicotiana tabacum L.) by agro-morphological traits and simple sequence repeat markers. Revista Ciência Agronômica, 44: 347-355.
7. Filiz E and Koç İ (2011). Molecular Markers in Plant Biotechnology. Journal of Agricultural Faculty of Gaziosmanpasa University, 8: 207-214.
8. Fricano A, Bakaher N, del Corvo M, Piffanelli P, Donini P, Stella A, Ivanov NV and Pozzi C (2012). Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm. BMC Genetic, 13: 1-18.

9. Keskin A, Koprulu TK, Bursali A, Ozsemir AC, Yavuz KE and Tekin S (2014). First record of Ixodes arboricola (Ixodida: Ixodidae) from Turkey with presence of Candidatus Rickettsia vini (Rickettsiales: Rickettsiaceae). Journal of Medical Entomology, 51: 864-7.
10. Kınay A (2014). Yield and quality properties in some oriental tobacco (Nicotiana tabacum L.) hybrids. University of Gaziosmanpaşa. Institute of Science, Dissertation, Tokat.
11. Moon HS, Nifong JM, Nicholson JS, Heineman A, Lion K, van der Hoeven R, Hayes AJ and Lewis RS (2009). Microsatellite-based analysis of tobacco (Nicotiana tabacum L.). Crop Science, 49: 2149-2159.
12. Nei M (1972). Genetic distance between populations. The American Naturalist, 106:283-292.
13. Peksüslü A, Yılmaz I, Inal A and Kartal H (2012). Tobacco genotypes. Journal of Aegean Agricultural Research Institute, 22: 82-90.
14. Tong Z, Yang Z, Chen X, Jiao F, Li X, Wu X, Gao Y, Xiao B and Wu W (2012). Large-scale development of microsatellite markers in Nicotiana tabacum and construction of a genetic map of flue-cured tobacco. Plant Breeding, 131: 674-680.
15. Tong Z, Xiao B, Jiao F, Fang D, Zeng J, Wu X, Chen, X, Yang J and Li Y (2016). Large-scale development of SSR markers in tobacco and construction of a linkage map in flue-cured tobacco. Breeding Science, 66: 381-390.
16. Yeh FC, Yang RC, Boyle TBJ, Ye ZH and Mao JX (1997). POPGENE the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre. https://sites.ualberta.ca/~fyeh/popgene_download.html. (Accessed to 17 January 2020).