Eradication of PVX, PVY, PVS, PVM and PLRV from Potato by Chemotherapy, Thermotherapy and Their Combinations

Öz

The study was carried out at Texas A&M University Potato Breeding and Variety Program in 2015, and the same study was repeated for the second time in 2016 for the reliability of the results. The five potato viruses chosen were PVX, PVY, PVS, PVM and PLRV because of their ease of inoculation by mechanical transmission. Five virus positive potato selections, virus that were obtained from the Texas A&M University Potato Breeding and Variety Program and Colorado State University Program, were used in this study. Control (tissue culture alone), chemotherapy, thermotherapy and combinations of chemotherapy+thermotherapy were used to eradicate the viruses. The control (tissue culture alone) application did not give successful results in virus eradication. Chemotherapy showed the greatest success in clearing PVM (75%-66.667%) in both years. Thermotherapy application showed the highest success in eradication of PLRV with 58.30% in the first year and 75.00% in the second year. Combining thermotherapy and chemotherapy was effective in eliminating both PVY and PLRV.

Anahtar Kelimeler

• Solanum tuberosum
• virus eradication
• chemotherapy
• thermotherapy

PVX, PVY, PVS, PVM ve PLRV'nin Patatesten Kemoterapi, Termoterapi ve Kemoterapi+Termoterapi ile Temizlenmesi

Öz

Çalışma 2015 yılında Texas A&M Üniversitesi Patates Islahı ve Çeşit Programında gerçekleştirilmiş olup, sonuçların güvenirliği için 2016 yılında aynı çalışma ikinci kez tekrarlanmıştır. Seçilen beş patates virüsü, mekanik aktarım yoluyla aşılama kolaylığı nedeniyle PVX, PVY, PVS, PVM ve PLRV idi. Bu çalışmada Texas A&M Üniversitesi Patates Yetiştirme ve Çeşit Programı ile Colorado Eyalet Üniversitesi Programından elde edilen virüs pozitif patates genotipleri kullanılmıştır. Virüsleri temizlemede sadece doku kültürü uygulaması olan kontrol ile kemoterapi, termoterapi ve kemoterapi+termoterapi kombinasyonları kullanılmıştır. Sadece doku kültürüne tabi tutulan kontrol uygulaması virüs temizleme de başarılı sonuç vermemiştir. Kemoterapi uygulaması en fazla başarıyı her iki yılda da PVM’nin (75%-66.667%) temizlenmesinde göstermiştir. Termoterapi uygulaması ise ilk yıl 58.30% ikinci yıl 75.00% olmak üzere en fazla başarıyı PLRV’nin temizlenmesinde göstermiştir. Termoterapi ve kemoterapi yöntemlerinin kombine edilerek birlikte uygulanması hem PVY hem de PLRV'yi ortadan kaldırmada oldukça etkili olmuştur.

Anahtar Kelimeler

• Solanum tuberosum
• virus temizleme
• kemoterapi
• termoterapi

Kaynakça

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