Molecular Characterization of Cylindrical Inclusion Protein Gene Regions of Turkish Zucchini yellow mosaic virus (ZYMV) Isolates

Öz

Zucchini yellow mosaic virus (ZYMV) is an economically important viral pathogen that causes intense mosaic symptoms and disfigurement in cucurbits. The aim of this study is to characterize ZYMV isolates obtained from Ankara, Antalya, Burdur, Konya, Karaman, Aksaray provinces of Turkey according to cylindrical inclusion (CI) protein sequences and to determine conserved areas on protein in Potyviruses. For this purpose, molecular studies and sequence analyzes were performed with primers specific to the CI protein region of collected cucurbit samples during 2019-2014 years. At the end of the study, the N terminus of Turkish ZYMV's CI protein 888 nucleotides long and 296 amino acids (aa) was amplified. Phylogenetic analysis of the nucleotide sequences of the CI region showed that the majority of isolates (40) belonged to a large molecular subgroup (A1) most common in Europe and the world, and three isolates (Y4, Y21, Y23) belonged to subgroup A5. Moreover, according to the coat protein nucleotide analysis, these three isolates (Y4, Y21, Y23) were grouped with the molecular subgroup A4, the group that emerged recently in Europe. The CI ZYMV nucleotide binding motif (NTBM) and RNA helicase activity site (five motifs) were conserved among isolates according to amino acid analysis.

Anahtar Kelimeler

• Cucurbites
• cylindrical inclusion
• helicase activity
• Phylogenetic analysis
• Sequence analyzes
• ZYMV

Türkiye ZYMV İzolatlarının CI Protein Gen Bölgelerinin Moleküler Karakterizasyonu

Öz

Kabak sarı mozaik virüsü (ZYMV), kabakgillerde yoğun mozaik semptomlarına ve şekil bozukluğuna neden olan ekonomik açıdan önemli bir viral etmendir. Bu çalışmanın amacı, Türkiye’de farklı illerden (Ankara, Antalya, Burdur, Konya, Karaman, Aksaray) elde edilen ZYMV izolatlarının silindirik inklüzyon (CI) protein dizilerine göre karakterize edilmesi ve Potyvirüslerde protein üzerinde korunan alanların belirlenmesidir. Bu amaçla CI protein bölgesine spesifik primerler ile moleküler çalışmalar ve dizi analizleri yapılmıştır. Çalışma sonunda, Türk ZYMV'nin CI proteini 888 nükleotid uzunluğunda ve 296 amino asit (aa) olan N uç kısmı çoğaltılmıştır. CI bölgesinin nükleotid dizilerinin filogenetik analizi, izolatların (40) çoğunluğunun Avrupa ve dünyada en yaygın olan büyük bir moleküler alt grubuna (A1) ve üç izolatın (Y4, Y21, Y23) A5 alt grubuna ait olduğunu göstermiştir. Ayrıca, kılıf proteini nükleotid analizine göre, bu üç izolat (Y4, Y21, Y23), Avrupa'da son zamanlarda ortaya çıkan grup olan A4 moleküler alt grubu ile gruplanmıştır. CI ZYMV nükleotid bağlama motifi (NTBM) ve RNA helikaz aktivite bölgesinin (beş motif) aa analizine göre izolatlar arasında korunduğu görülmüştür.

Anahtar Kelimeler

• Filogenetik analiz
• Sekans analizi
• Kabakgiller

Kaynakça

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