Bioinformatical Evaluation of PPARA and PPARG Candidate Genes for Milk Quality Characteristics in Turkish Saanen Goats

Yıl 2022, , 77 - 84, 31.08.2022

Funda Ataç , Yakut Gevrekçi , Çiğdem Takma , Fatma Akkaya , Mustafa Can Yılmaz , Tarik Ayyılmaz , Dilsat Yegenoglu

https://doi.org/10.55507/gopzfd.1131221

Öz

In this study, genetic information obtained in terms of milk yield and milk quality characteristics was used in order to increase the accuracy of breeding selection of Turkish Saanen goats. For this purpose, DNA sequencing of PPARG and PPARA genes from Turkish Saanen goats were compared with the sequences in gene databases and single nucleotide polymorphisms were examined. A relationship was found between some quality parameters (fat, protein and somatic cell count) of milk measurements of Turkish Saanen goats and PPARA and PPARG gene variants. Single nucleotide polymorphisms were detected in the exons and introns of the relevant gene variants, however, these polymorphisms did not have a statistically significant effect on quality parameters (p>0.05).

Anahtar Kelimeler

Bioinformatics, Candidate genes, PPARA, PPARG, Turkish Saanen goat

Destekleyen Kurum

Ege Üniversitesi Bilimsel Araştırma Projeleri (BAP)

Proje Numarası

FGA-2019-20593

Teşekkür

The authors gratefully acknowledge the financial support of this research by Ege University Scientific Research Projects Coordination (BAP)

Kaynakça

• Abousoliman, I., Reyer, H., Oster, M., Murani, E., Mohamed I., & Wimmers, K. (2021). Genome-Wide SNP Analysis for Milk Performance Traits in Indigenous Sheep: A Case Study in the Egyptian Barki Sheep. Animals, 11(6):1671. https://doi.org/10.3390/ani11061671
• Ataç, F.E., Takma, Ç., Gevrekçi, Y., Yeğenoğlu, E.D., & Örgel, S. (2018). Hair Goat Breeding in Ecological Environment and Products: A Review. International Echology 2018 Symposium, P:1058, 19-23 June 2018, Kastamonu Turkey. www.ecology2018.com
• Benjelloun, B., Alberto, F. J., Streeter, I., Boyer, F., Coissac, E., Stucki, S., Mohammed ,B., Mustapha, I., Mouad, C., Abdelmajid, B., Kevin, B., Adriana, A., Stefan, E., Abdelkader, C., Laura, C., Paul, F., Stephane, J., Pierre, T., Farnçois, P., & Nextgen, C. (2015). Characterizing neutral genomic diversity and selection signatures in indigenous populations of Moroccan goats (Capra hircus) using WGS data. Frontiers in Genetics, 6:107. doi: 10.3389/fgene.2015.00107.
• Bentley150 Operator’s Manual. (1999). A guide for installation, operation, and maintenance. Bentley Instruments, Inc. Chaska, Minnesota, USA.
• Bionaz, M., Hausman, G.J., & Mandard, S. (2013). Physiological and nutritional roles of PPAR across species. PPAR Research, 1-3. D’Agostino, G., LaRana, G., Russo, R., Sasso, O., Iacono, A., & Esposito, E. (2007). Acute intracerebroventricular administration of palmitoylethanolamide, an endogenous peroxisome proliferator-activated receptor-alpha agonist, modulates carrageenan- induced paw edema in mice. Journal of Pharmacology and Experimental Therapeutics, 322:1137-43.
• Ebrahimi-Mamaeghani, M., Mohammadi, S., Arefhosseini, S. R., Fallah, P., & Bazi, Z. (2015). Adiponectin as a potential biomarker of vascular disease. Vascular Health and Risk Management, 11: 55-70.
• Fan, Y. Y., Sen Zan, l., Fu, C. Z., Tian, W.Q., Wang, H. B., Liu, Y. Y., & Xin, Y. P. (2011).Three novel SNPs in the coding region of PPARγ gene and their associations with meat quality traits in cattle. Molecular Biology Reports, 38 (2011), pp. 131-137.
• Ferreira, L., Soaresa, M.A.M., Rodrigues, M.T., de Araujoc, J.L.S., Melod, A.L.P., Gasparinoe, E., & Garciaa, O.S.R. (2020). UCP2 and PPARG gene polymorphisms and their association with milk yield and composition traits in goats. Small Ruminant Research Volume, 192, November 2020, 106210
• Haenlein, G.F.W. (2007). About the evolution of goat and sheep milk production. Small Ruminant Research, 68: 3-6.
• Hou, J.X., An, X.P., Song, Y.X., Wang, J.G., Ma, T., Han, P., Fang, F., & Cao, B.Y. (2013). Combined effects of four SNPs within goat PRLR gene on milk production traits. Gene, 529, 276-281.
• Işık, R. (2017). Saanen Keçilerinde β- Laktoglobulin (β-LG) Geni Ekzon 7 Polimorfizmi ve Süt Verimi ile İlişkisi. Tekirdağ Ziraat Fakültesi Dergisi, 35-40.
• Kersten, S. (2014). Integrated physiology and systems biology of PPARα. Molecular Metabolism, 3 (4): 354-371.
• Lee, J.Y., Ha, J.J., Park, Y.S., Yi, J.K., Lee, S., Mun, S., Han, K., Kim, J.J., Kim, H.J., & Oh, D.Y. (2016). Relationship between single nucleotide polymorphisms in the peroxisome proliferator-activated receptor gamma gene and fatty acid composition in Korean native cattle. Asian-Australasian Journal of Animal Sciences, 29 pp. 184-194,
• Ferreira, L., Soares, M.A.M., Rodrigues, M.T., de Araujo, J.L.S., de Melo, A.L.P., Gasparino, E., Garcia, & O.S.R. (2020). UCP2 and PPARG gene polymorphisms and their association with milk yield and composition traits in goats. Small Ruminant Research, Volume 192, 106210.
• Martin, H. (2010). Role of PPAR-gamma in inflammation. Prospects for therapeutic intervention by food components. Mutation Research, 690 (1–2): 57-63.
• McKenna, N,J., & O'Malley, B.W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators. Cell, vol. 108, no. 4, pp. 465-474.
• Najafi, MN., Mortazavi, SA., Koocheki, A., Khorami, J., & Rekik, B. (2009). Fat and protein contents, acidity and somatic cell counts in bulk milk of holstein cows in the Khorasan Razavi province, Iran. International Journal of Dairy Technology, 62(1): 19-26.
• Pramod, R.K., Velayutham, D., Zachariah, A., Zachariah, A., Santhosh, S., Iype, S., Dhinoth Kumar, B., Gupta, R., & Thomas, G. (2018). The complete mitochondrial genome of Indian cattle [Bos indicus]. Mitochondrial DNA Part B. 3: 207–208.
• Shi, H.B., Luo, J., Yao, D.W., Zhu, J.J., Xu, H.F., Shi, H.P., & Loor J.J. (2013a). Peroxisome proliferator-activated receptor-Y stimulates the synthesis of monounsaturated fatty acids in dairy goat mammary epithelial cells via the control of stearoyl-coenzyme A desaturase. Journal of Dairy Science, 96:7844-7853.
• Shi, H., Luo, J., Zhu, J., Li, J., Sun, Y., Lin, X., Zhang, L., Yao, D., & Shi, H. (2013b). PPAR γ regulates genes involved in triacylglycerol synthesis and secretion in mammary gland epithelial cells of dairy goats. PPAR research, 2013: 310948. https://doi.org/10.1155/2013/310948
• Shipman, K.E., Strange, R.C., & Ramachandran, S. (2016). Use of fibrates in the metabolic syndrome: A review. World Journal of Diabetes, 7: 74–88.
• Somacount150 Operator’s Manual. (1998). A Guide for installation, operation, and maintenance. Bentley Instruments, Inc. Revised edition. Printed in the USA.
• Yakan, A., Özkan, H., Eraslan, Ş., Ünal, N., & Özbeyaz, C. (2018). Gene expression levels in some candidate genes for mastitis resistance, milk yield, and milk quality of goats reared under different feeding systems. Turkish Journal of Veterinary and Animal Sciences, 42: 18-28.