Tetranychus urticae Koch (Acari: Tetranychidae)'de Wolbachia Endosimbiyontu ve Spirodiclofen Direnci Arasındaki İlişki

Yıl 2023, , 115 - 124, 03.01.2024

Betül Bal , Sibel Yorulmaz

https://doi.org/10.55507/gopzfd.1339608

Öz

Tetranychus urticae Koch (Acari: Tetranychidae), dünyada birçok kültür bitkisi çeşidinde ekonomik kayıplara neden olan önemli bir zararlıdır. Bu çalışmada, T. urticae' de spirodiclofen direnci ile Wolbachia varlığı arasındaki ilişkinin belirlenmesi amaçlanmıştır. Bu nedenle, T. urticae' nin hem Wolbachia ile enfekteli olan (GSS) hem de enfekteli olmayan (GSSN) popülasyonlarında eş zamanlı spirodiclofen seleksiyonu yapılmıştır. T. urticae' de lethal konsantrasyon (LC) değerlerini belirlemek için kuru rezidü yöntemi kullanılmıştır. Biyoassay denemelerde akarın larva dönemine uygulanmıştır. Lethal konsantrasyon çalışmaları 7 doz +1 kontrol ve 3 tekerrürlü olarak yapılmıştır. 7. gün sonunda ölü-canlı sayımı yapılarak direnç oranları belirlenmiştir. T. urticae'nin Wolbachia ile enfekteli olan son seleksiyonunda 23 kat spirodiclofen direnci belirlenirken, Wolbachia ile enfekteli olmayan son seleksiyonda 103 kat direnç belirlenmiştir. Wolbachia varlığının, Wolbachia ile enfekte olmayan tüm seleksiyon popülasyonlarında, Wolbachia ile enfekte olmuş seleksiyon popülasyonlarına kıyasla oldukça düşük olduğu belirlenmiştir. Sonuç olarak T. urticae'de spirodiclofen direnci ile Wolbachia endosymbiont arasında negatif bir ilişki olabileceği ve bu ilişki kapsamında esteraz enziminin direnç gelişiminde etkili olabileceği düşünülmektedir.

Anahtar Kelimeler

esteraz, LC, akarisit, Tetranychus urticae, Wolbachia

The Relationship between Spirodiclofen Resistance and Wolbachia Endosymbiont in Tetranychus urticae Koch (Acari: Tetranychidae)

Öz

Tetranychus urticae Koch (Acari: Tetranychidae) is an important pest that causes economic losses in many varieties of cultivated plants around the world. In this study, it was aimed at determiningthe relationship between spirodiclofen resistance in T. urticae and the presence of Wolbachia. Therefore, simultaneous selection of spirodiclofen was performed in both Wolbachia infected (GSS) and uninfected (GSSN) populations of T. urticae. The dry residue method was used to determine lethal concentration (LC) values in T. urticae. Bioassay experiments were applied to the larval stage of the mite. The LC value studies were established as 7 doses +1 control and 3 replications. Dead-alive counts were made at the end of the 7th day and resistance ratios were determined. In the last selection of T. urticae with Wolbachia infection, 23-fold spirodiclofen resistance was determined, and in the last selection without Wolbachia infection, 103-fold resistance was determined. The presence of Wolbachia was found to be quite low in all Wolbachia-uninfected selection populations compared to Wolbachia-infected populations. As a result, it is thought that there may be a negative relationship between spirodiclofen resistance and Wolbachia endosymbiont in T. urticae, and that esterase enzyme may have an effect on the development of resistance within the scope of this relationship.

Anahtar Kelimeler

esterase, LC, acaricide, Tetranychus urticae, Wolbachia

Kaynakça

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