Assessment of InDel Variations in CPT1A and PRDM6 Genes Across Three Anatolian Goat Populations

Yıl 2024, Cilt: 41 Sayı: 3, 265 - 270, 31.12.2024

Yusuf Aktaş , Veli Atmaca , Eymen Demir , Taki Karslı

https://doi.org/10.55507/gopzfd.1568134

Cited By: 1

Öz

IIdentifying candidate genes associated with growth traits in livestock and incorporating them into Marker Assisted Selection (MAS) studies, in addition to classical selection methods, can increase genetic progress and thus profitability. Carnitine Palmitoyl Transferase 1a (CPT1a) and PR domain family 6 (PRDM6) are among the candidate genes reported to be associated with growth traits in goats. This study aimed to identify polymorphisms in the CPT1a and PRDM6 genes related to growth traits in Kıl (HAI), Honamlı (HNM), and Kabakulak (KBK) goats, and to discuss their potential use in MAS studies. The II genotype of the CPT1a gene was not found in the HNM or KIL goats, whereas the KBK population included all three genotypes (II, ID, and DD). The frequency of the ID genotype ranged from 0.25 (HAI) to 0.40 (KBK), while the DD genotype frequency ranged from 0.37 (KBK) to 0.75 (HAI). For the PRDM6 gene, the KBK population was found to be monomorphic, containing only the DD genotype. Although the II genotype was not detected in the HNM and HAI breeds, the ID genotype frequencies were calculated as 0.05 and 0.07, respectively. The study's findings point out that the CPT1a gene can be used in MAS studies for HNM, KBK, and HAI goats, whereas the PRDM6 gene cannot be utilized.

Anahtar Kelimeler

CPT1a , PRDM6 , goat , indel , polymorphism

Etik Beyan

This study is reviewed and approved by the Eskişehir Osmangazi University Animal Experiments Local Ethic Committee (Protocol No: HAYDEK-968/2023).

Destekleyen Kurum

The Scientific Research Projects Coordination of Eskişehir Osmangazi University

Proje Numarası

FLO/2023/3009

Teşekkür

In This research was financially supported by the Scientific Research Projects Coordination of Eskişehir Osmangazi University (Project Number: FLO/2023/3009).

Üç Anadolu Keçisi Populasyonunda CPT1A ve PRDM6 Genlerindeki InDel Varyasyonlarının Değerlendirilmesi

Öz

Çiftlik hayvanlarında büyüme özellikleri ilişkili aday gen bölgelerinin belirlenerek klasik seleksiyon çalışmalarına ek olarak yapılacak Marker Destekli Seleksiyon (MAS) çalışmalarında kullanımı genetik ilerleme hızını ve karlılığı artırılabilir. Keçilerde büyüme özellikleri ile ilişkili olduğu bildirilen aday genler arasında Karnitin Palmitoil Transferaz 1a (CPT1a) ve PR domain family 6 (PRDM6) genleri de vardır. Gerçekleştirilen çalışmada Kıl (HAI), Honamlı (HNM) ve Kabakulak (KBK) keçilerinde büyüme özellikleri ile ilişkili CPT1a ve PRDM6 genleri üzerindeki indel varyantların neden olduğu polimorfizmlerin araştırılması ve MAS çalışmalarında kullanım olanaklarının tartışılması amaçlanmıştır. CPT1a geni için HNM ve KIL keçilerinde II genotipi tespit edilemezken, KBK populasyonunda her üç genotip de (II, ID ve DD) tespit edilmiştir. ID genotip frekansının 0.25 (HAI) ile 0.40 (KBK) aralığında, DD genotip frekansının ise 0.37 (KBK) ile 0.75 (HAI) aralığında değiştiği tespit edilmiştir. PRMD6 geni için KBK populasyonu monomorf (yalnızca genotip DD) bulunmuştur. HNM ve HAI ırklarında II genotipi tespit edilemezken, ID genotip frekansları sırasıyla 0.05 ve 0.07 olarak hesaplanmıştır. Çalışmadan elde edilen sonuçlar HNM, KBK ve HAI keçilerinde CPT1a geninin MAS çalışmalarında kullanılabileceğine ancak PRDM6 geninin kullanılamayacağına işaret etmektedir.

Anahtar Kelimeler

CPT1a , PRDM6 , keçi , indel , polimorfizm

Proje Numarası

FLO/2023/3009

Kaynakça

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