Assessment of winter survival in barley (Hordeum vulgare L.) genotypes using molecular markers and some physiological traits

Öz

To evaluate the freezing resistance and genetic diversity in barley physiological traits, molecular markers and their relationship in 20 barley genotypes were assessed in field and greenhouse condition. The analysis of variance showed a significant difference among studied genotypes. The effect of acclimation temperature on prolin content, quantum efficiency of photosystem II, chlorophyll and soluble sugars content were studied as physiological traits. Freezing treatments were -4, -7, -10, -13 and -16°C temperatures that applied in a 3 replicated randomized complete block design. Then their lethal temperature at which 50% of plant were died (LT50) was determined. To estimate FSI (Field Survival Index) index, the 20 genotypes were cultured in a separate experiment on field with 3 replications. The results showed negative significant correlation (-0.601) between field survival index and LT50. Cluster analysis using physiological traits, genotypes of F-A1-1, F-A1-2, F-A2-11, F-GRB-85-5, Sahra, Sahand, Dasht and Makouei were categorized in a distinct group and had a high FSI and low LT50. Makouei cultivar having LT50=-17.66°C and the highest percentage of winter survival in the field, was the most resistant genotype. 10 ISSR markers from 35 primers sequences were selected and used. These 9 ISSR primers produced 50 polymorphic bands. PIC and MI average index for all primers were 0.37 and 1.72 respectively. Cluster analysis of molecular data using Jaccard similarity coefficient categorized the genotypes to four distinct groups. Associations between molecular markers and traits were assessed by multiple regression analysis. Some informative markers related to FSI and also LT50 was determined. So it may be possible to use these markers for selection of resistant lines or genotypes in breeding programs.

Anahtar Kelimeler

• Barley, Freezing resistance, genetic diversity, molecular marker, Physiological traits

Moleküler Markörler ve Fizyolojik Özellikler Kullanılarak Arpa (Hordeum Vulgare L.) Genotiplerinde Kış Canlılığının Değerlendirilmesi

Öz

To evaluate the freezing resistance and genetic diversity in barley physiological traits, molecular markers and their relationship in 20 barley genotypes were assessed in field and greenhouse condition.

Anahtar Kelimeler

• Barley, Freezing resistance, genetic diversity, molecular marker, Physiological traits

Kaynakça

1. Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free prolin for water-stress studies. Plant and Soil, 39: 205- 207.
2. Bhardway R and Singhal G (1981). Effect of water stress on photochemical activity of chloroplasts during greening etiolated barley seedlings. Plant Cell Physiol, 22: 15516
3. Breton G, Danyaluk J, Ouellet F, Sarhan F (2000). Biotechnological application of plant freezing associated proteins. Biotechnol. Ann Rev, 6: 57-82.
4. Bridger GM, Falk DE, Mc Kersie BD, Smith DL (1996). Crown freezing tolerance and field winter survival of winter cereals in Eastern Canada. Crop Sci, 35: 15015
5. Fernandez ME, Figueiras AM, Benito C (2002). The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetics diversity among barly cultivars whith known origin. Theor Appl Genet, 104: 845- 851.
6. Fowler DB (1982). Date of seedling, fall growth and winter survival of winter wheat and rye. Agron J, 74: 1060106
7. Fowler DB (2002). Winter Cereal Production. Crop Development Center, University of Saskatchewan, Canada. URL:http://www.usask.ca/agriculture/cropsci/winter_cer eals/
8. Fowler DB, Gusta LV, Tyler NJ (1981). Selection for winter hardiness in wheat . III. Screening methods. Crop Sci, 21: 896- 900.

9. Gebhardt C, Ballvora A, Walkemeier B, Oberhagemann P, Schuler K (2004)Assessing genetic potential in germplasm collections of crop plants by marker-trait association: A case study for potatoes with quantitative variation of resistance to late blight and maturity type. Mol Breed, 13: 93-102.
10. Gusta LV, Fowler DB, Tyler NJ (1982). Factors influencing hardening and survival in winter wheat. In: Li PH, Sakai A (eds) Plant cold hardiness and freezing stress: mechanisms and crop implication Academic New York 2:23–40.
11. Irigoyen JJ, Emerich DW, Sanchez-Diaz M (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa L.) plants. Physiol Plant, 84: 55-60.
12. Lander ES and Botstein D (1987). Mapping mandelian factors underlying quantitative trait using RFLP linkage maps. Genet, 121: 179-186.
13. Limin AE and Fowler DB (1988). Cold hardiness expression in interspecific hybrids and amphiploids of the Triticeae. Genome, 30: 361- 365.
14. Livingston DP and Premakumar R (2002). Apoplastic carbohydrates do not account for differences in freezing tolerance of two winter oat cultivars that have been second phase cold hardened. Cereal research Commun, 30: 375- 381.
15. Mahfoozi S, Roustaii M, Ansari- Maleki Y (2005). Determination of low temperature tolerance in some bread wheat, durum wheat and barley genotypes. Seed and Plant, 21: 467-483.
16. Naghavi A, Sofalian O, Asghari A, Sedghi M (2010). Relation between freezing tolerance and seed storage proteins in winter bread wheat (Triticum aestivum L.). Tur J Field Crops, 15: 154-158.
17. Petcu E and Terbea M (1995). Prolin content and the conductivity test as screening methods for frost tolerance of winter wheat. Plant Physiol, 21: 3-11.
18. Prasil IT, Prasilova P, Marik P (2007). Comparative study of direct and indirect evaluations of frost tolerance inbarley. Field Crops Res, 102: 1- 8.
19. Rizza F, Pagani D, Stance AM, Cattivelli L (2001). Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and spring oats. Plant Breed, 120: 389-396.
20. Rong-hu AL, Pei-guo G, Baum M, Grando S, Ceccarelli S (2006). Evaluation of cholorofyll content and fluorescence parameters as indicators of drought tolerance in barley. Agric Sci China, 5: 751-757.
21. Saghai-Maroof M, Soliman A, Jorgensen K, Allard RA (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. PNAS, 81: 8014- 8018.
22. Terzopoulos PJ and Bebeli PJ (2008). Genetic diversity analysis of Mediterranean faba bean (Vicia faba L.) with ISSR markers. Field Crops Res, 108: 39-44.
23. Vagujfalvi A, Kerepsi J, Galiba G, Tischner T, Sutka J (1999). Frost Hardiness depending on carbohydrate changes during cold acclimation in wheat. Plant Sci, 144: 85- 92.