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Eradication of PVX, PVY, PVS, PVM and PLRV from Potato by Chemotherapy, Thermotherapy and Their Combinations

Yıl 2021, Cilt: 38 Sayı: 3, 117 - 122, 31.12.2021
https://doi.org/10.13002/jafag4787

Öz

The study was carried out at Texas A&M University Potato Breeding and Variety Program in 2015, and the same study was repeated for the second time in 2016 for the reliability of the results. The five potato viruses chosen were PVX, PVY, PVS, PVM and PLRV because of their ease of inoculation by mechanical transmission. Five virus positive potato selections, virus that were obtained from the Texas A&M University Potato Breeding and Variety Program and Colorado State University Program, were used in this study. Control (tissue culture alone), chemotherapy, thermotherapy and combinations of chemotherapy+thermotherapy were used to eradicate the viruses. The control (tissue culture alone) application did not give successful results in virus eradication. Chemotherapy showed the greatest success in clearing PVM (75%-66.667%) in both years. Thermotherapy application showed the highest success in eradication of PLRV with 58.30% in the first year and 75.00% in the second year. Combining thermotherapy and chemotherapy was effective in eliminating both PVY and PLRV.

Kaynakça

  • Beemster ABR. and Bokx J.A. de (1987). Survey of properties and symptoms. In: Viruses of Potato and Seed Potato Production, eds. J.A. de Bokx and J.P.H.van der Want, 84-113. Wageningen, the Netherlands: Pudoc.
  • Cassells AC and Long RD (1982). The elimination of potato viruses X, Y, S, and M in meristem and explant cultures of potato in the presence of Virazole. Potato Research 25: 165-173.
  • Cassells AC, Long RD and Austin S (1983). The effect of virazole in tobacco petiole cultures, leaf disks and isolated protoplasts in relation to its antiviral activity against potato virus Y, tobacco mosaic virus and cucumber mosaic virus. In: Plant tissue culture in relation to biotechnology, eds. A.C. Cassells and J.A. Kavanagh, 72-83. Proceedings of the Royal Irish Academy.
  • Clark MF and Adams AN (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475-483.
  • Chahardehi A, Rakhshandehroo F, Mozafari J and Mousavi L (2016). Efficiency of a chemo-thermotherapy technique for eliminating Arabis mosaic virus (ArMV) and Prunus necrotic ringspot virus (PNRSV) from in vitro rose plantlets. Journal of Crop Protection 5 (4): 497–506.
  • Chen WQ and Sherwood JL (1991). Evaluation of tip culture, thermotherapy and chemotherapy for elimination of peanut mottle virus from Arachis hypogaea. Journal of Phytopathology 132:230-236.
  • Cordeiro L (2003). Stock indexing and potato virus Y elimination from potato plants cultivated in vitro stock indexing and potato virus Y elimination. Scientia Agricola 60 (3): 525–530.
  • Dodds JH, Lizarraga R, Griffiths H and Slack SA (1989). Methods of virus eradication. 1977 Lima: International Potato Center.
  • Dunbar KB, Pinnow DL, Morris JB and Pittman RN (1993). Virus elimination from interspecific Arachis hybrids. Plant Disease77: 517-520.
  • Fletcher PJ, Fletcher JD and Cross RJ (1998). Potato germplasm: In vitro storage and virus reduction. New Zeland Journal of Crop and Horticultural Science 26; 249-252.
  • Griffiths HM, Slack SA and Dodds, JH (1990). Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Canadian Journal of Botany 68:1515-1521.
  • Hansen AJ (1985). An end to the dilemma-virus-free all the way. Hortscience 20: 852-859.
  • Hansen AJ and Lane WD 1(985). Elimination of apple chlorotic leafspot virus from apple shoot cultures by ribavirin. Plant Disease 69:134-135.
  • Hsu YH, Annamalai P, Lin CS, Chen YY, Chang WC and Lin NS (2000). A sensitive method for detecting bamboo mosaic virus (BaMV) and establishment of BaMV free meristem-tip cultures. Plant Pathology 49: 101-107.
  • Hu G, Dong Y, Zhang Z, Fan, X, Ren F and Zhou J (2015). Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Plant Cell, Tissue and Organ Culture 121 (2): 435–443.
  • Hull R (2002). Matthew’s Plant Virology. London: Academic Press.
  • Jeffries CJ (1998). Potato. FAOIIPGRI Technical Guidelines for the Safe Movement of Germplasm, No. l9. Rome: FAO & IPGRI.
  • Khurana SMP (2004) Potato Viruses and Their Management. In: SAMH Naqvi (ed) Diseases of Fruits and Vegetables: Diagnosis and Management, Kluwer Academic, Dordrecht, Boston and London, 2004, 389-440.
  • Klein RE and Livingston CH (1982). Eradication of potato virus X from potato by ribavirin treatment of cultured potato shoot tips. American Potato Journal 59:359-365.
  • Knapp E, Hanzer V, Weiss H, da Camara Machado A, Wang Q, Weiss B, Katinger H and Laimer da Camara Machado M (1995). Distribution of apple chlorotic leaf spot virus in apple shoots cultivated in vitro. ACTA HORT. 386: 187-194.
  • Lawson C, Kaniewski W, Haley L, Rozman R, Newell C. Sanders P and Turner NE (1990). Engineering resistance to mixed virus infection in a commercial potato cultivar: Resistance to potato virus X and potato virus Yin transgenic Russet Burbank. Biotechnology 8:127-134.
  • Lee RF, Nylandg G and Lowes K (1987). Chemotherapy of cherry buckskin and peach yellow leafroll diseases: an evaluation of two-tetracycline formulations and methods of application. Plant Dis. 71: 119-121.
  • Lizarraga R, Panta A, Jayasinghe U and Dodds J (1991). Tissue culture for elimination of pathogens. Lima: International Potato Center, 21p.
  • Loebenstein G, Berger PH, Brunt AA and Lawson RH (2001). Virus and Virus-like Diseases of Potatoes and Production of Seed- Potatoes, Kluwer Academic Publishers, pp. 69 – 75.
  • Lozoya-Saldana H and Dawson WO (1982). The use of constant and alternating temperature regimes and tissue culture to obtain PVS-free potato plants. Am Potato J. 59: 221-229.
  • Lozoya-Saldana H, Merlin-Lara O (1984) Thermotherapy and tissue culture for elimination of Potato virus X(PVX) in Mexican potato cultivars resistant to late blight. Am Potato J 61:735–739.
  • MacDonald DM 1973. Heat treatment and meristem culture as a means of freeing potato varieties from viruses X and S. Potato Res. 16: 263-269.
  • Mancino LJC and Agrios GN (1984). Effects of antiviral compounds on symptoms and infectivity of cowpea chlorotic mosaic virus. Plant Disease 68:219-222.
  • Mellor FC and Stace-Smith R (1970). Virus strain differences in eradication of potato virus X and S. Phytopathology 60: 1587-1590.
  • Nascimento LC, Pio-Ribeiro G, Willadino L and Andrade, G.P (2003). Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro. Scientia Agricola, 60(3): 525-530.
  • Quak F (1987). Therapy of individual plants. In: Viruses of Potatoes and Seed-Potato Production. (eds) J.A. de Bokx and J.P.H. van der Want, pp. 151-161. Pudoc, Wageningen, The Netherlands.
  • Rowe CR (1993). Potato Health Management. Department of Plant Pathology. Ohio State University, USA.
  • Salazar LF (1996). Potato Viruses and their Control, 214 pp. Intern. Potato Center, Lima, Peru.
  • Sangar RBS, Agrawal HO and Nagaich BB (1988). Studies on the translocation of potato viruses X and Y in potatoes. Indian Phytopathology, V.41, p.327-331.
  • Simpkins I, Walkey DGA and Neely HA (1981). Chemical suppression of virus in cultured plant tissues. Annals of Applied Biology, V.99, P.161- 169.
  • Singh, B. 2015. Effect of antiviral chemicals on in vitro regeneration response and production of PLRV-free plants of potato. Journal of Crop Science and Biotechnology 18 (5): 341–348.
  • Spooner DM, Ghislain M, Simon R, Jansky SH and Gavrilenko T (2014). The New York Botanical Garden. Bot. Rev. (2014) 80:283-383. DOI 10.1007/s12229-014-9146-y.
  • Stace-Smith R and Mellor FC (1968). Eradication of potato virus X and S by thermotherapy and axillary bud culture. Phytopathology 53:199-203.
  • Tomlinson JA (1982). Chemotherapy of plant viruses and virus diseases. In: Pathogens, vectors and plant disease approaches to control. Edited by K.E Harris and K. Maramorosch. Academic Press, New York. pp. 23-44.
  • Vicente M and De Fazio G (1987). Perspectivas em quimioterapia de vírus de plantas. Fitopatologia Brasileira, V.12, p.21-26.
  • Walkey DGA (1980). Production of virus free plants by tissue culture. In: Tissue culture methods for plant pathologists. Edited by Ingram, D.S. and J.E Helgeson. Blackwell Scientific Publications, Oxford. pp. 109-119.
  • Wang B, Ma Y, Zhang Z, Wu Z, Wu Y, Wang Q and Li M (2011). Potato viruses in China. Crop Protection 30 (9): 1117–1123
  • Wambugu EM, Secor GA and Gudmestad NC (1985). Eradication of potato virus Y and S from potato by chemotherapy of cultured axillary bud tips. Am Potato J 62:667-672.
  • Waswa M, Kakuhenzire R and Ochwo-Ssemakula M (2017). Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks. African Journal of Plant Science 11 (3): 61–70.
  • Yang L, Nie B, Liu J and Song B (2013). A reexamination of the effectiveness of ribavirin on eradication of viruses in potato plantlets in vitro using ELISA and Quantitative RT-PCR. American Journal of Potato Research 91: 304–311.
  • Zapata C, Miller JC JR and Smith RH (1995). An in vitro procedure to eradicate potato viruses X, Y, and S from Russet Narkotah and two of its strains, In Vitro Cell. Dev. Biol.-Plant, 31: 153-159

PVX, PVY, PVS, PVM ve PLRV'nin Patatesten Kemoterapi, Termoterapi ve Kemoterapi+Termoterapi ile Temizlenmesi

Yıl 2021, Cilt: 38 Sayı: 3, 117 - 122, 31.12.2021
https://doi.org/10.13002/jafag4787

Öz

Çalışma 2015 yılında Texas A&M Üniversitesi Patates Islahı ve Çeşit Programında gerçekleştirilmiş olup, sonuçların güvenirliği için 2016 yılında aynı çalışma ikinci kez tekrarlanmıştır. Seçilen beş patates virüsü, mekanik aktarım yoluyla aşılama kolaylığı nedeniyle PVX, PVY, PVS, PVM ve PLRV idi. Bu çalışmada Texas A&M Üniversitesi Patates Yetiştirme ve Çeşit Programı ile Colorado Eyalet Üniversitesi Programından elde edilen virüs pozitif patates genotipleri kullanılmıştır. Virüsleri temizlemede sadece doku kültürü uygulaması olan kontrol ile kemoterapi, termoterapi ve kemoterapi+termoterapi kombinasyonları kullanılmıştır. Sadece doku kültürüne tabi tutulan kontrol uygulaması virüs temizleme de başarılı sonuç vermemiştir. Kemoterapi uygulaması en fazla başarıyı her iki yılda da PVM’nin (75%-66.667%) temizlenmesinde göstermiştir. Termoterapi uygulaması ise ilk yıl 58.30% ikinci yıl 75.00% olmak üzere en fazla başarıyı PLRV’nin temizlenmesinde göstermiştir. Termoterapi ve kemoterapi yöntemlerinin kombine edilerek birlikte uygulanması hem PVY hem de PLRV'yi ortadan kaldırmada oldukça etkili olmuştur.

Kaynakça

  • Beemster ABR. and Bokx J.A. de (1987). Survey of properties and symptoms. In: Viruses of Potato and Seed Potato Production, eds. J.A. de Bokx and J.P.H.van der Want, 84-113. Wageningen, the Netherlands: Pudoc.
  • Cassells AC and Long RD (1982). The elimination of potato viruses X, Y, S, and M in meristem and explant cultures of potato in the presence of Virazole. Potato Research 25: 165-173.
  • Cassells AC, Long RD and Austin S (1983). The effect of virazole in tobacco petiole cultures, leaf disks and isolated protoplasts in relation to its antiviral activity against potato virus Y, tobacco mosaic virus and cucumber mosaic virus. In: Plant tissue culture in relation to biotechnology, eds. A.C. Cassells and J.A. Kavanagh, 72-83. Proceedings of the Royal Irish Academy.
  • Clark MF and Adams AN (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475-483.
  • Chahardehi A, Rakhshandehroo F, Mozafari J and Mousavi L (2016). Efficiency of a chemo-thermotherapy technique for eliminating Arabis mosaic virus (ArMV) and Prunus necrotic ringspot virus (PNRSV) from in vitro rose plantlets. Journal of Crop Protection 5 (4): 497–506.
  • Chen WQ and Sherwood JL (1991). Evaluation of tip culture, thermotherapy and chemotherapy for elimination of peanut mottle virus from Arachis hypogaea. Journal of Phytopathology 132:230-236.
  • Cordeiro L (2003). Stock indexing and potato virus Y elimination from potato plants cultivated in vitro stock indexing and potato virus Y elimination. Scientia Agricola 60 (3): 525–530.
  • Dodds JH, Lizarraga R, Griffiths H and Slack SA (1989). Methods of virus eradication. 1977 Lima: International Potato Center.
  • Dunbar KB, Pinnow DL, Morris JB and Pittman RN (1993). Virus elimination from interspecific Arachis hybrids. Plant Disease77: 517-520.
  • Fletcher PJ, Fletcher JD and Cross RJ (1998). Potato germplasm: In vitro storage and virus reduction. New Zeland Journal of Crop and Horticultural Science 26; 249-252.
  • Griffiths HM, Slack SA and Dodds, JH (1990). Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Canadian Journal of Botany 68:1515-1521.
  • Hansen AJ (1985). An end to the dilemma-virus-free all the way. Hortscience 20: 852-859.
  • Hansen AJ and Lane WD 1(985). Elimination of apple chlorotic leafspot virus from apple shoot cultures by ribavirin. Plant Disease 69:134-135.
  • Hsu YH, Annamalai P, Lin CS, Chen YY, Chang WC and Lin NS (2000). A sensitive method for detecting bamboo mosaic virus (BaMV) and establishment of BaMV free meristem-tip cultures. Plant Pathology 49: 101-107.
  • Hu G, Dong Y, Zhang Z, Fan, X, Ren F and Zhou J (2015). Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Plant Cell, Tissue and Organ Culture 121 (2): 435–443.
  • Hull R (2002). Matthew’s Plant Virology. London: Academic Press.
  • Jeffries CJ (1998). Potato. FAOIIPGRI Technical Guidelines for the Safe Movement of Germplasm, No. l9. Rome: FAO & IPGRI.
  • Khurana SMP (2004) Potato Viruses and Their Management. In: SAMH Naqvi (ed) Diseases of Fruits and Vegetables: Diagnosis and Management, Kluwer Academic, Dordrecht, Boston and London, 2004, 389-440.
  • Klein RE and Livingston CH (1982). Eradication of potato virus X from potato by ribavirin treatment of cultured potato shoot tips. American Potato Journal 59:359-365.
  • Knapp E, Hanzer V, Weiss H, da Camara Machado A, Wang Q, Weiss B, Katinger H and Laimer da Camara Machado M (1995). Distribution of apple chlorotic leaf spot virus in apple shoots cultivated in vitro. ACTA HORT. 386: 187-194.
  • Lawson C, Kaniewski W, Haley L, Rozman R, Newell C. Sanders P and Turner NE (1990). Engineering resistance to mixed virus infection in a commercial potato cultivar: Resistance to potato virus X and potato virus Yin transgenic Russet Burbank. Biotechnology 8:127-134.
  • Lee RF, Nylandg G and Lowes K (1987). Chemotherapy of cherry buckskin and peach yellow leafroll diseases: an evaluation of two-tetracycline formulations and methods of application. Plant Dis. 71: 119-121.
  • Lizarraga R, Panta A, Jayasinghe U and Dodds J (1991). Tissue culture for elimination of pathogens. Lima: International Potato Center, 21p.
  • Loebenstein G, Berger PH, Brunt AA and Lawson RH (2001). Virus and Virus-like Diseases of Potatoes and Production of Seed- Potatoes, Kluwer Academic Publishers, pp. 69 – 75.
  • Lozoya-Saldana H and Dawson WO (1982). The use of constant and alternating temperature regimes and tissue culture to obtain PVS-free potato plants. Am Potato J. 59: 221-229.
  • Lozoya-Saldana H, Merlin-Lara O (1984) Thermotherapy and tissue culture for elimination of Potato virus X(PVX) in Mexican potato cultivars resistant to late blight. Am Potato J 61:735–739.
  • MacDonald DM 1973. Heat treatment and meristem culture as a means of freeing potato varieties from viruses X and S. Potato Res. 16: 263-269.
  • Mancino LJC and Agrios GN (1984). Effects of antiviral compounds on symptoms and infectivity of cowpea chlorotic mosaic virus. Plant Disease 68:219-222.
  • Mellor FC and Stace-Smith R (1970). Virus strain differences in eradication of potato virus X and S. Phytopathology 60: 1587-1590.
  • Nascimento LC, Pio-Ribeiro G, Willadino L and Andrade, G.P (2003). Stock indexing and Potato virus Y elimination from potato plants cultivated in vitro. Scientia Agricola, 60(3): 525-530.
  • Quak F (1987). Therapy of individual plants. In: Viruses of Potatoes and Seed-Potato Production. (eds) J.A. de Bokx and J.P.H. van der Want, pp. 151-161. Pudoc, Wageningen, The Netherlands.
  • Rowe CR (1993). Potato Health Management. Department of Plant Pathology. Ohio State University, USA.
  • Salazar LF (1996). Potato Viruses and their Control, 214 pp. Intern. Potato Center, Lima, Peru.
  • Sangar RBS, Agrawal HO and Nagaich BB (1988). Studies on the translocation of potato viruses X and Y in potatoes. Indian Phytopathology, V.41, p.327-331.
  • Simpkins I, Walkey DGA and Neely HA (1981). Chemical suppression of virus in cultured plant tissues. Annals of Applied Biology, V.99, P.161- 169.
  • Singh, B. 2015. Effect of antiviral chemicals on in vitro regeneration response and production of PLRV-free plants of potato. Journal of Crop Science and Biotechnology 18 (5): 341–348.
  • Spooner DM, Ghislain M, Simon R, Jansky SH and Gavrilenko T (2014). The New York Botanical Garden. Bot. Rev. (2014) 80:283-383. DOI 10.1007/s12229-014-9146-y.
  • Stace-Smith R and Mellor FC (1968). Eradication of potato virus X and S by thermotherapy and axillary bud culture. Phytopathology 53:199-203.
  • Tomlinson JA (1982). Chemotherapy of plant viruses and virus diseases. In: Pathogens, vectors and plant disease approaches to control. Edited by K.E Harris and K. Maramorosch. Academic Press, New York. pp. 23-44.
  • Vicente M and De Fazio G (1987). Perspectivas em quimioterapia de vírus de plantas. Fitopatologia Brasileira, V.12, p.21-26.
  • Walkey DGA (1980). Production of virus free plants by tissue culture. In: Tissue culture methods for plant pathologists. Edited by Ingram, D.S. and J.E Helgeson. Blackwell Scientific Publications, Oxford. pp. 109-119.
  • Wang B, Ma Y, Zhang Z, Wu Z, Wu Y, Wang Q and Li M (2011). Potato viruses in China. Crop Protection 30 (9): 1117–1123
  • Wambugu EM, Secor GA and Gudmestad NC (1985). Eradication of potato virus Y and S from potato by chemotherapy of cultured axillary bud tips. Am Potato J 62:667-672.
  • Waswa M, Kakuhenzire R and Ochwo-Ssemakula M (2017). Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks. African Journal of Plant Science 11 (3): 61–70.
  • Yang L, Nie B, Liu J and Song B (2013). A reexamination of the effectiveness of ribavirin on eradication of viruses in potato plantlets in vitro using ELISA and Quantitative RT-PCR. American Journal of Potato Research 91: 304–311.
  • Zapata C, Miller JC JR and Smith RH (1995). An in vitro procedure to eradicate potato viruses X, Y, and S from Russet Narkotah and two of its strains, In Vitro Cell. Dev. Biol.-Plant, 31: 153-159
Toplam 46 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makaleleri
Yazarlar

Yasin Karan 0000-0003-2354-8995

Douglas C. Scheurıng Bu kişi benim 0000-0003-3939-3916

Angel L. Chappell Bu kişi benim 0000-0001-9353-2759

Yayımlanma Tarihi 31 Aralık 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 38 Sayı: 3

Kaynak Göster

APA Karan, Y., Scheurıng, D. C., & Chappell, A. L. (2021). Eradication of PVX, PVY, PVS, PVM and PLRV from Potato by Chemotherapy, Thermotherapy and Their Combinations. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 38(3), 117-122. https://doi.org/10.13002/jafag4787