DVD-SNO® Takviye Edici Gıda Ürününün Sıçanlarda Akut Sub-akut Toksisitesinin Araştırılması

Yıl 2020, Cilt: 2 Sayı: 3, 51 - 58, 31.12.2020

Mustafa Yaşar , Güliz Saraç Ali Gök , Kağan Ağan , Pınar Ağyar Yoldaş , Neslihan Şirin

Öz

Geleneksel olarak sıklıkla kullanılan tıbbi bitkilerin bilinçsiz kullanımı bazı komplikasyonlara neden olabilmektedir. Bitkilerin toksisite testlerinin detaylı olarak araştırılması bitkisel ürünlerin olası etkilerinin belirlenmesi, takviye edici gıdaların üretiminde uygun dozun ayarlanması açısından önem arz etmektedir. DVD-SNO®, devedikeni tohumu (Silybum marianum), biberiye (Rosmarinus officinali), zerdeçal (Curcuma longa), şahtere (Fumaria officinalis), hindiba (Cichorium intybus) ve sinirli ot (Plantago majör) bitkilerinin kombinasyonuyla hazırlanmış bitkisel bir gıda takviyesidir. DVD-SNO® içeriğindeki bu bitkilerin geleneksel olarak kombine halde ya da ayrı ayrı kullanımları yaygındır ve sağlık için faydalı oldukları düşünülmektedir.

Bu çalışmada Remember Regeneration Tedavide (RTM) kullanılan DVD-SNO® ürününün akut ve sub-akut toksisitesinin belirlenmesi amaçlanmıştır. Deney gruplarında 250-300 g ağırlığında sekiz aylık Wistar albino dişi sıçanlar kullanılmıştır. Çalışma ISO 10993-11, ISO 10993-2 ve ISO 10993-12 uluslararası test standartlarından yararlanılarak tasarlanmıştır. Kontrol grubuna salin, deney gruplarına 13,3 mg/mL DVD-SNO® takviye edici gıda ürünü gavaj yoluyla verilmiştir.

Uygulama süreleri sonunda (akut toksisite için 1 gün ve sub-akut toksisite için 7 gün, sub-akut -sonrası toksisite için 14 gün) hayvanlardan kan ve doku örnekleri alındı. Kan örnekleri biyokimyasal ve hematolojik parametreler için analiz edildi. Kalp, karaciğer, akciğer, böbrek ve dalak dokularının histopatolojisi incelendi. İncelenen tüm parametreler, akut ve sub-akut toksisite açısından kontrol ve uygulama grupları arasında önemli bir farklılık taşımadığı görüldü. Sonuç olarak, sıçanlar üzerinde yapılan bu çalışmada DVD-SNO® ürününün günlük kullanım dozu akut ve sub-akut toksisite göstermemiştir.

Anahtar Kelimeler

Akut toksisite, Subakut toksisite, Gıda takviyesi, Silybum marianum, Rosmarinus officinalis, Curcuma longa, Fumaria officinalis, Cichorium intybus, Plantago majör

Investigation of Acute Sub-acute Toxicity of DVD-SNO® Supplement Food Product in Rats

Öz

Unconscious use of traditionally used medicinal plants can cause some complications. A detailed investigation of the toxicity tests of plants is important in terms of determining the possible effects of herbal products, and adjusting the appropriate dose in the production of supplements. DVD-SNO® is a herbal food supplement prepared with the combination of thistle seeds (Silybum marianum), rosemary (Rosmarinus officinalis), turmeric (Curcuma longa), fumitory (Fumaria officinalis), chicory (Cichorium intybus) and plantain (Plantago major) plants. These herbs, which are contained in DVD-SNO®, are commonly used in combination or separately and are considered to be beneficial for health. In this study, it was aimed to determine the acute and sub-acute toxicity of DVD-SNO® product used in Remember Regeneration Therapy Method (RTM). Eight months old Wistar female rats weighing 250-300 g were used in the experimental groups. The study was designed using international test standards ISO 10993-11, ISO 10993-2 and ISO 10993-12. Saline was given to the control group and 13.3 mg / mL DVD-SNO® supplement food product via gavage. At the end of the treatment period (1 day for acute toxicity and 7 days for sub-acute toxicity, 14 days for post sub-acute toxicity) blood and tissue samples were collected from the animals. Blood samples were analyzed for biochemical and hematological parameters. Histopathology of the heart, liver, lung, kidney and spleen tissues has been investigated. It was observed that there was no significant difference between the control and administration groups in terms of all parameters examined, acute and sub-acute toxicity. As a result, in this study on rats, the daily use dose of DVD-SNO® product did not show acute and sub-acute toxicity.

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