Immobilization of GOx on Trp / Trp-Fc Functionalized Nanospheres: Improved of Reusability and Stability

Yıl 2017, Cilt: 30 Sayı: 4, 114 - 122, 11.12.2017

Nurdan Kurnaz Yetim , Elvan Hasanoğlu Özkan , Nurşen Sarı

Öz

In the study, new nanospheres support
containing ferrocene were synthesized and characterized, then the properties
of nanospheres as biocatalysis were researched. Novel support was prepared
from (Aminomethyl)polystyrene (AMP), tryptophan (Trp) and ferrocene aldehyde (Fc)  and the
enzymatic properties of immobilized 
glucose oxidase enzyme (GOx) was investigated. Modified
polystyrene was characterized IR spectra, Gel permeation chromatography and
Scanning electron microscopy. Ferrocene-tagged nanospheres-GOx
shows high reusability and storage capacity and fast incubation time
determination of glucose. After 12 months, immobilized GOx to the AMP-Trp
and AMP-Trp-Fc retained ca 20 % and
40 % for their original activity, respectively. High activities were found for
modified polymers AMP-Trp-Fc retains more than 35 % of the
initial activity after fifteenth successive cycles, which is a perfect performance.

Anahtar Kelimeler

Biocatalysis, ferrocene, glucose oxidase, nanospheres

Kaynakça

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