Optimization of Endo-Pectinase and Pectin Lyase Production from Wheat Bran by Bacillus pumilus using Response Surface Methodology

Year 2021, Volume: 34 Issue: 2, 335 - 353, 01.06.2021

Özlem Tepe , Arzu Yadigar Dursun

https://doi.org/10.35378/gujs.808326

Cited By: 3

Abstract

Wheat bran is one of the most used agricultural residues for enzyme synthesis. It has a high organic content; thus, it causes environmental pollution. In this study, wheat bran was selected as a carbon source and it was supplemented with yeast extract and ammonium sulphate. Endopectinase and pectin lyase production by Bacillus pumilus was performed in a batch system. RSM was applied to examine the effects of the wheat bran, yeast extract and ammonium sulphate concentrations on the synthesis of enzymes and the dosages of these nutrients were optimized. According to the model design, the optimum conditions were 4.74% w/v wheat bran, 0.12% ammonium sulphate and 0.12% yeast extract. The high values of R2 and R2 adj indicated that the fitted model showed good agreement with the predicted and actual values. In conclusion, these studies revealed that wheat bran can be used for the production of endo-pectinase and pectin lyase enzymes based on high enzyme activities.

Keywords

Endo-pectinase, Optimization, Pectin lyase, Response surface methodology (RSM), Wheat bran

Supporting Institution

Scientific Research Projects Management Unit of Firat University

Project Number

FÜBAP 1674

Thanks

This study was funded by Scientific Research Projects Management Unit of Firat University (FUBAP) (Grant no. FÜBAP 1674). This paper includes a part of Ph.D thesis data of Ozlem TEPE.

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