Multifunctional Gelatin Hydrogel Microspheres Delivering Phages and Basic Fibroblast Growth Factor

Abstract

Bu çalışmanın amacı, enfekte yaraların iyileşmesinde kullanılmak üzere çok fonksiyonlu farmasötikler olarak fajları (antibakteriyel ajan olarak) ve/veya temel fibroblast büyüme faktörünü (anjiyojenik faktör olarak) taşıyan – kontrollu salan jelatin hidrojel mikroküreleri (GEL'ler) hazırlamaktır. Hem konak hem de hedef olarak E.coli kullanılarak T4 fajları çoğaltılmış ve aktivite testleri yapılmıştır. Farklı çapraz bağlanma derecelerine sahip GEL'ler iki aşamalı bir proses ile bazik ve/veya asidik jelatin kullanılarak hazırlanmıştır. Jeller farklı miktarlarda glutaraldehit ile çapraz bağlanmıştır. Asidik ve enzimatik bozunmalar incelenmiştir. Bu mikrokürelerin içine T4 fajları ve/veya temel fibroblast büyüme faktörü yüklenmiş ve salım kinetikleri ve modları araştırılarak optimize edilmiştir

Keywords

• Jelatin hidrojel mikroküreler
• T4 fajı
• temel fibroblast büyüme faktörü
• kontrollü salım

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