Screening of Chemical Profiles of Echinophora chrysantha Extracts by RP-HPLC and Evaluation of Antiproliferative Activities

Abstract

The purpose of this study was to ascertain the phytochemical composition and antiproliferative properties of methanolic extract made from the roots, branches, leaves, and flowers of Echinophora chrysantha (EC). Phytochemical content was determined using RP-HPLC. Sixteen metabolites were identified as a result of RP-HPLC examination of EC extracts. Using the XTT technique, antiproliferative activity tests were examined on HT29 and A549 cell lines. All extracts strongly inhibited the examined cell lines. In particular, the branch extract shown outstanding antiproliferative activity against A549 (IC50:7.40 μg/mL) and HT-29 (IC50:1.70 μg/mL). Echinophora chrysantha's traditional use and therapeutic usefulness are further reinforced by the identification of its metabolites and assessment of its bioactivity.

Keywords

• Colon cancer
• HPLC analysis
• Lung cancer
• Echinophora cinerea

References

1. B. S. Chhikara, K. Parang. Chemical Biology LETTERS Global Cancer Statistics 2022: the trends projection analysis. Chem. Biol. Lett. Chem. Biol. Lett, (2023) 1–16.
2. T. Bakheet, N. Al-Mutairi, M. Doubi, W. Al-Ahmadi, K. Alhosaini, F. Al-Zoghaibi. A Computational Recognition Analysis of Promising Prognostic Biomarkers in Breast, Colon and Lung Cancer Patients. Int. J. Mol. Sci., 26 (2025) .
3. L. Vázquez-Iglesias, G. M. Stanfoca Casagrande, D. García-Lojo, L. Ferro Leal, T. A. Ngo, J. Pérez-Juste, R. M. Reis, K. Kant, I. Pastoriza-Santos. SERS sensing for cancer biomarker: Approaches and directions. Bioact. Mater., 34 (2024) 248–268.
4. U. Anand, A. Dey, A. K. S. Chandel, R. Sanyal, A. Mishra, D. K. Pandey, V. De Falco, A. Upadhyay, R. Kandimalla, A. Chaudhary, J. K. Dhanjal, S. Dewanjee, J. Vallamkondu, J. M. Pérez de la Lastra. Cancer chemotherapy and beyond: Current status, drug candidates, associated risks and progress in targeted therapeutics. Genes Dis., 10 (2023) 1367–1401.
5. A. Lichota, K. Gwozdzinski. Anticancer activity of natural compounds from plant and marine environment. Int. J. Mol. Sci., 19 (2018), DOI 10.3390/ijms19113533.
6. N. Ramalingum, M. F. Mahomoodally. The therapeutic potential of medicinal foods. Adv. Pharmacol. Sci., (2014).
7. P. Hashemi, M. M. Abolghasemi, A. R. Ghiasvand, S. Ahmadi, H. Hassanvand, A. Yarahmadi. A comparative study of hydrodistillation and hydrodistillation-solvent microextraction methods for identification of volatile components of Echinophora cinerea. Chromatographia, 69 (2009) 179–182.
8. A. Delazar, S. M. Yari, N. Chaparzadeh, S. Asnaashari, L. Nahar, N. Delazar, S. D. Sarker. Chemical Composition, Free-Radical-Scavenging and Insecticidal Properties, and General Toxicity of Volatile Oils Isolated from Various Parts of Echinophora orientalis. J. Essent. Oil-Bearing Plants, 18 (2015) 1287–1297.

9. A. Doğan, G. Bulut, E. Tuzlacı, İ. Şenkardeş. A review of edible plants on the Turkish Apiaceae species. İstanbul Ecz. Fak. Derg. / J. Fac. Pharm. Istanbul, 44 (2014) 251–262.
10. B. Ezatpour, M. Bahmani, M. Azami, F. Kheirandish, M. Rafieian-kopaei. The In Vitro Effects of Echinophora Cinerea on Cell Line, Giardia Lamblia Cyst, and Giardia Muris. 3 (2018) 3–8.
11. K. H. C. Baser, T. Özek, B. Demirçakmak, A. Biçakçi, H. Malyer. Essential oil of echinophora chrysantha freyn et Sint. J. Essent. Oil Res., 8 (1996) 433–434.
12. K. H. C. Baser, M. Kürkcüoglu, H. Malyer, A. Bicakci. Essential oils of six echinophora species from Turkey. J. Essent. Oil Res., 10 (1998) 345–351.
13. H. Aksit, Z. Aksit, S. Simsek, A. Aydin, M. A. Yilmaz, A. Kandemir, E. Koksal. LC-MS/MS profiling phytochemical content of Echinophora chrysantha (Apiaceae) and antiproliferative, antioxidant activity. Pharm. Pharmacol. Int. J., 10 (2022) 190–194.
14. A. K. Yüksel, E. Dikici, M. Yüksel, M. Işik, F. Tozoğlu, E. Köksal. Phytochemicals Analysis and Some Bioactive Properties of Erica manipuliflora Salisb. (EMS); Antibacterial, Antiradical and Anti-lipid Peroxidation. Iran. J. Pharm. Res., 20 (2021) 422–434.
15. Y. K. Cen, J. G. Lin, J. Y. Wang, Z. Q. Liu, Y. G. Zheng. Colorimetric assay for active biomass quantification of Fusarium fujikuroi. J. Microbiol. Methods, 155 (2018) 37–41.
16. D. A. Scudiero, R. H. Shoemaker, K. D. Paull, A. Monks, S. Tierney, T. H. Nofziger, M. J. Currens, D. Seniff, M. R. Boyd. Evaluation of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug Sensitivity in Culture Using Human and Other Tumor Cell Lines. Cancer Res., 48 (1988) 4827–4833.
17. X. Liu, D. P. Rodeheaver, J. C. White, A. M. Wright, L. M. Walker, F. Zhang, S. Shannon. A comparison of in vitro cytotoxicity assays in medical device regulatory studies. Regul. Toxicol. Pharmacol., 97 (2018) 24–32.
18. S. Meschini, M. Marra, A. Calcabrini, E. Monti, M. Gariboldi, E. Dolfini, G. Arancia. Role of the lung resistance-related protein (LRP) in the drug sensitivity of cultured tumor cells. Toxicol. Vitr., 16 (2002) 389–398.
19. N. Çiftçi. Oksidatif Stresin Kanserdeki Rolü: Antioksidanlar Kanser Progresyonunun Yakıtı Olabilir mi? Ahi Evran Tıp Derg., 1 (2017) 8–13.
20. Â. Luís, F. Domingues, A. P. Duarte. Bioactive compounds, RP-HPLC analysis of phenolics, and antioxidant activity of some Portuguese shrub species extracts. Nat. Prod. Commun., 6 (2011) 1863–1872.
21. A. Bouyahya, N. El Menyiy, L. Oumeslakht, A. El Allam, A. Balahbib, A. Rauf, N. Muhammad, E. Kuznetsova, M. Derkho, M. Thiruvengadam, M. A. Shariati, N. El Omari. Preclinical and clinical antioxidant effects of natural compounds against oxidative stress-induced epigenetic instability in tumor cells. Antioxidants, 2021, 10.
22. S. Noor, T. Mohammad, M. A. Rub, A. Raza, N. Azum, D. K. Yadav, M. I. Hassan, A. M. Asiri. Biomedical features and therapeutic potential of rosmarinic acid. Arch. Pharm. Res., 45 (2022) 205–228.
23. Y. Lu, Y. Hong, T. Zhang, Y. Chen, Z. Wei, C. Gao. Rosmarinic acid exerts anti-inflammatory effect and relieves oxidative stress via Nrf2 activation in carbon tetrachloride-induced liver damage. Food & Nutrition Research, 66 (2022), 10-29219.
24. S. Moradkhani, A. M. Ayatollahi, M. Ghanadian, M. R. Moin, M. Razavizadeh, M. Shahlaeif. Phytochemical analysis and metal-chelation activity of Achillea tenuifolia Lam. Iran. J. Pharm. Res., 11 (2012) 177–183.
25. D. Kim, M. L. Mollah, K. Kim. Induction of apoptosis of SW480 human colon cancer cells by (-)-epicatechin isolated from Bulnesia sarmienti. Anticancer Res., 32 (2012) 5353–5362.
26. A. Kowalczyk, C. I. G. Tuberoso, I. Jerković. The Role of Rosmarinic Acid in Cancer Prevention and Therapy: Mechanisms of Antioxidant and Anticancer Activity. Antioxidants, 13 (2024) 1–18.
27. S. Ijaz, J. Iqbal, B. A. Abbasi, Z. Ullah, T. Yaseen, S. Kanwal, T. Mahmood, S. Sydykbayeva, A. Ydyrys, Z. M. Almarhoon, J. Sharifi-Rad, C. Hano, D. Calina, W. C. Cho. Rosmarinic acid and its derivatives: Current insights on anticancer potential and other biomedical applications. Biomed. Pharmacother., 162 (2023) 114687.
28. S. R. Alizadeh, M. A. Ebrahimzadeh. O-Glycoside quercetin derivatives: Biological activities, mechanisms of action, and structure–activity relationship for drug design, a review. Phyther. Res., 36 (2022) 778–807.
29. S. A. Arshad Husain Rahmani, Ali Yousif Babiker. Hesperidin A Bioflavonoid in Cancer Therapy.pdf. Molecules, (2023), 28.
30. A. Othman, A. M. M. Jalil, K. K. Weng, A. Ismail, N. A. Ghani, I. Adenan. Epicatechin content and antioxidant capacity of cocoa beans from four different countries. African J. Biotechnol., 9 (2010) 1052–1059.
31. P. C. D. P. Vasconcelos, L. N. Seito, L. C. Di Stasi, C. Akiko Hiruma-Lima, C. H. Pellizzon. Epicatechin used in the treatment of intestinal inflammatory disease: An analysis by experimental models. Evidence-based Complement. Altern. Med.,(2012).
32. J. Shay, H. A. Elbaz, I. Lee, S. P. Zielske, M. H. Malek, M. Hüttemann. Molecular mechanisms and therapeutic effects of (-)- epicatechin and other polyphenols in cancer, inflammation, diabetes, and neurodegeneration. Oxid. Med. Cell. Longev., (2015).
33. F. Pereyra-Vergara, I. M. Olivares-Corichi, A. G. Perez-Ruiz, J. P. Luna-Arias, J. R. García-Sánchez. Apoptosis induced by (−)-epicatechin in human breast cancer cells is mediated by reactive oxygen species. Molecules, 25 (2020).