Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester

Tuğçe Deniz Karaca; Hüseyin Balcı; Arzu Aysan; Yusuf Sert

doi:10.15671/hjbc.1684535

Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester

Abstract

The increasing the importance of synthesizing compounds with high biocompatibility, low cytotoxicity, and advanced antibacterial properties is evident in current research. L-3,4-Dihydroxyphenylalanine (L-DOPA) is a crucial drug widely used in the treatment of Parkinson's disease. In this study, we investigated the antimicrobial and cytotoxic effects of L-DOPA and its water-soluble derivative, L-DOPA methyl ester. The antimicrobial effects of L-DOPA and L-DOPA Methyl Ester were tested on both Gram-negative and Gram-positive bacteria. Additionally, the impact of L-DOPA and its ester on cell viability was assessed using the MTT assay on cancerous and non-cancerous cell lines. The study revealed that both substances were effective against Gram-positive bacteria but not Gram-negative bacteria. Specifically, the growth of Gram-positive bacteria was inhibited by both compounds, with L-DOPA showing greater efficacy. We also observed that L-DOPA and its ester exhibit anti-cancer activity. Furthermore, the molecular docking mechanism between the three targets (2DS2, 4URO and 1M17) and the compounds was investigated using Autodock Vina. The findings show that the L-DOPA molecule is more appropriately attached to the target receptors than the L-DOPA methyl ester molecule. As a result, its inhibition is greater. The results obtained are new and it is thought that these results of the study will contribute to the development of new synthesizable drug studies.

Keywords

References

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Details

Primary Language

English

Subjects

Cell Development, Proliferation and Death

Journal Section

Research Article

Authors

Tuğçe Deniz Karaca ^*
0000-0002-7471-1759
Türkiye

Hüseyin Balcı
0000-0002-8778-4586
Türkiye

Arzu Aysan
0000-0001-7217-3347
Türkiye

Yusuf Sert
0000-0001-8836-8667
Türkiye

Publication Date

October 1, 2025

Submission Date

April 28, 2025

Acceptance Date

September 10, 2025

Published in Issue

Year 2025 Volume: 53 Number: 4

DOI

https://doi.org/10.15671/hjbc.1684535

IZ

https://izlik.org/JA25SG53UF

Cite

RIS / Bibtex

APA

Karaca, T. D., Balcı, H., Aysan, A., & Sert, Y. (2025). Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester. Hacettepe Journal of Biology and Chemistry, 53(4), 33-46. https://doi.org/10.15671/hjbc.1684535

AMA

1.Karaca TD, Balcı H, Aysan A, Sert Y. Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester. HJBC. 2025;53(4):33-46. doi:10.15671/hjbc.1684535

Chicago

Karaca, Tuğçe Deniz, Hüseyin Balcı, Arzu Aysan, and Yusuf Sert. 2025. “Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester”. Hacettepe Journal of Biology and Chemistry 53 (4): 33-46. https://doi.org/10.15671/hjbc.1684535.

EndNote

Karaca TD, Balcı H, Aysan A, Sert Y (October 1, 2025) Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester. Hacettepe Journal of Biology and Chemistry 53 4 33–46.

IEEE

[1]T. D. Karaca, H. Balcı, A. Aysan, and Y. Sert, “Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester”, HJBC, vol. 53, no. 4, pp. 33–46, Oct. 2025, doi: 10.15671/hjbc.1684535.

ISNAD

Karaca, Tuğçe Deniz - Balcı, Hüseyin - Aysan, Arzu - Sert, Yusuf. “Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester”. Hacettepe Journal of Biology and Chemistry 53/4 (October 1, 2025): 33-46. https://doi.org/10.15671/hjbc.1684535.

JAMA

1.Karaca TD, Balcı H, Aysan A, Sert Y. Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester. HJBC. 2025;53:33–46.

MLA

Karaca, Tuğçe Deniz, et al. “Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester”. Hacettepe Journal of Biology and Chemistry, vol. 53, no. 4, Oct. 2025, pp. 33-46, doi:10.15671/hjbc.1684535.

Vancouver

1.Tuğçe Deniz Karaca, Hüseyin Balcı, Arzu Aysan, Yusuf Sert. Analyses and Comparisons of the Antimicrobial-Cytotoxic Effects and Molecular Docking Study of L-DOPA and L-DOPA Methyl Ester. HJBC. 2025 Oct. 1;53(4):33-46. doi:10.15671/hjbc.1684535